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TITLE: Identification and Isolation of Neural Stem Cells and Neurosphere-Initiating Cells INVENTORS: Sean Morrison and John Mich TECHNOLOGY: Biological UTSD: 2752 SUMMARY: This IPQ disclosed a method to identify and isolate simultaneously both neural stem cells (NSCs) as well as neurosphere-initiating cells (NICs) (two mutually exclusive populations) from adult mouse forebrain using a mix of commercially available antibodies and flow cytometry. From adult mouse lateral ventricle subventricular zone (SVZ), NICs are isolated and are highly mitotic in vivo and short-lived based on Ascl1CreERT2 and Dlx1CreERT2 fate-mapping. In contrast, a quiescent population of pre-GEPCOT cells could not form neurospheres but express the stem cell markers and they are long-lived in vivo. While GEPCOT NICs can be ablated by temozolomide (alkylating chemotherapy drug) treatment, pre GEPCOT cells survive and repopulate the SVZ. These two distinct cell populations, once isolated, can be directly studied in various assays of neural stem cell function, gene expression, etc. Current isolation method involves special mouse strains with fluorescent markers. It will take months to interbreed two fluorescent mutant strains into one of interest. This technique might also be applicable to human brain samples in the future. Please contact the Office for Technology Development for more details: Phone: 214-648-1816 Email: [email protected] Please reference UT Southwestern Case Number: 2752
