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Supplementary figure 4 a b Pixels / 2h1/2 Cell movement 150 100 *** 50 0 -50 -100 -150 -200 -250 c e Control Netrin1 Netrin1+ UNC5B-Fc d Supplementary figure 4. Aortic ring assays. a: overview of the culture. Scale bar, 0.5cm. b: quantification of cell movement. The distance of cell movement (pixel/2h1/2) was measured for each cell filmed (n=6 control, 8 Netrin-1, 16 Netrin-1/UNC5B-Fc). The mean±s.e.m. of all cells for each treatment is plotted. Reverse movement is denoted -. c: see movie1, control endothelial cells. d: see movie2, cells exposed to a gradient of pre-clustered Netrin-1/UNC5B-Fc (arrow). Movement of the two leading cells was quantified. e: see movie3, two cells exposed to a gradient of Netrin-1. Note retraction (movie3).