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MAUREEN CAROLINE McCANN
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Date of Birth:
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British
16th March 1966
505 Calvert Lane, Lafayette, IN47905
+1 765 429 4967
+1 765 496 1779
+1 765 496 1496
[email protected]
Qualifications:
1984-1987
Churchill College, University of Cambridge, UK
M. A. Natural Sciences
1987-1990
Department of Cell Biology, John Innes Centre, Norwich NR4 7UH, UK
Ph. D. Botany, University of East Anglia
Title of dissertation: Architecture of the plant extracellular matrix
Supervisor: Professor Keith Roberts
Employment:
2010 – present Director, Energy Center, Discovery Park, Purdue University
2010 - present Professor, Department of Biological Sciences, Purdue University
2009 - present Director, DOE-EFRC Center for direct catalytic conversion of biomass to biofuels,
Discovery Park, Purdue University
2007 - present Assistant Head, Department of Biological Sciences, Purdue University, Indiana.
2003-2010
Associate Professor, Department of Biological Sciences, Purdue University,
Indiana.
1995-2003
Royal Society University Research Fellowship
Department of Biological Sciences, University of East Anglia, and
Project Leader
Department of Cell and Developmental Biology, John Innes Centre, UK
1994-1995
Senior Scientific Officer
BBSRC/ Unilever LINK initiative for cell wall research
Department of Cell Biology, John Innes Centre, UK
1990-94
Higher Scientific Officer
BBSRC/ Unilever LINK initiative for cell wall research
Department of Cell Biology, John Innes Centre, UK
Honours and Appointments:
2010
NSF panel member: Integrative Organismal Systems
2010
DOE Knowledgebase Workshop participant, Chair, Plant biology section.
2009
Purdue’s Seeds of Success Award (for grants over $1M)
2008
Leadership Award, Special University Merit Award
2008-present Monitoring Editor, Plant Physiology
2008-present Monitoring Editor, Global Change Biology -Bioenergy
2007
Scientific Advisory Board Member for 11th International Cell Wall Meeting,
Copenhagen
2007
Review panel member: Stanford University Global Climate and Energy Program
2006-present Advisory Board Member for EPOBIO, a European consortium on useful products
from plants
2005
Purdue’s Seeds of Success Award (for grants over $1M)
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2005
2004-2007
2001
2000-2007
1998
1995
Department of Energy Committee of Visitors (Energy Biosciences) (April 5-8,
2005)
NSF panel member: Cellular Organization
International participant to US Department of Energy grant review panel
Editorial board member of Planta
Co-organizer, 8th International Cell Wall Meeting
First Walter Pilnik award for research into pectins
Research Interests:
We recognize the 40 or so different plant cell types by their characteristic shapes and sizes.
However, without its cell wall, the naked protoplast is spherical and is not capable of dividing,
growing, or becoming specialized for function. Molecules of the cell wall provide mechanical
strength, regulate porosity, and control cell-cell adhesion. The functions of the wall are not only
mechanical but also biological. Like the animal extracellular matrix, plant cell walls are a source
of signaling molecules that elicit a range of cell behaviors, committing the cell to particular
developmental programs. The goal of my research is to understand how the molecular
machinery of the plant cell wall contributes to cell growth and specialization, and thus to the
final stature and form of plants.
The plant cell wall is a highly organized composite of many different polysaccharides, proteins,
and aromatic substances that undergo dynamic changes during cell division, expansion and
differentiation. However, it has been difficult to ascribe specific functions to these molecules. As
part of an NSF-funded genomics project (see http://cellwall.genomics.purdue.edu), we have
used a high-through-put infrared spectroscopic screen to identify a broad-based collection of
mutant plants with variation in cell wall amount, composition or molecular architecture. In this
Purdue-centered collaboration with Dr Nicholas Carpita, Department of Botany and Plant
Pathology, we have screened populations of over 900 homozygous cell-wall-related mutant lines
of Arabidopsis, about one-quarter of which have alterations in cell wall composition or
architecture. We are using these materials to define the relationship between genetically-defined
changes in plant cell wall-related proteins (biosynthetic, hydrolytic, and structural) and cell wall
molecular architecture and structural properties and to examine the downstream consequences
for cell growth and differentiation.
Both final cell size and rate of cell expansion are constrained by the cell wall. I have proposed
that the wall-loosening mechanisms that permit cell growth to occur are regulated by the
biophysical properties of a network of pectic polysaccharides. Pectins are some of the most
structurally complex biomolecules known, comprise 20 to 50% of the mass of dicotyledonous
cell walls, and are cross-linked together and to other cell wall molecules in a variety of ways. In
order to elucidate the role of pectin in cell elongation, my lab uses mutants and transgenic
approaches to generate Arabidopsis plants of altered pectic composition. For example, we have
identified a 7-member gene family in Arabidopsis, the MYST gene family, that encode
rhamnogalacturonan (RG) lyases, an enzyme activity more commonly associated with plant
pathogens for cell wall hydrolysis. RG-lyases are enzymes that specifically recognize and cleave
glycosidic bonds between L-rhamnose and D-galacturonic acid residues in the backbone of RG-I
molecules. Mutation and over-expression of MYST gene family members each have dramatic
impacts on plant growth. Our data indicate crucial functions for MYST proteins and their
substrate in plant development.
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Plant cell walls are the source of lignocellulosic biomass, an untapped and sustainable resource
for biofuels production. We require tremendous increases in agricultural productivity to meet
the competing demands of food, feed and now biofuels without increasing our agronomic
footprint. Using a toolkit of plant genetics and molecular biology, we aim to optimize dedicated
bioenergy crops by gaining genetic control of the amount, composition and structure of cell
walls for their end-use as feedstocks in production of energy-rich biofuels. A frontier in energy
research is to develop technologies that maximize the energy and carbon efficiencies of biofuel
production by the rational and synergistic design of both physical and chemical conversion
processes and the biomass itself. I lead an Energy Frontier Research Center focused on the direct
catalytic conversion of biomass to biofuels, and I am building an interdisciplinary program to
tailor biomass for its end-use in thermal or catalytic conversion pathways for energy-rich fuels
and other bio-based products.
Key research achievements:
 A widely cited model of plant cell wall architecture for dicotyledonous species (McCann
and Roberts 1991, Carpita and McCann 2000, 2010 submitted, reproduced in
Molecular Biology of the Cell and several other textbooks).
 Pioneering applications of novel microscopy (fast-freeze, deep-etch, rotary-shadowed
replicas) (Carpita et al 2001, Wells et al 1994, McCann et al 1993, 1990) and
spectroscopy techniques (Fourier transform infrared microspectroscopy) to plant cell
walls (Penning et al 2009, Chen et al 1998, 1997a, b, Kemsley et al 1994, Sene et al 1994,
McCann et al 1992)
 Identifying functions for pectins related to biophysical properties of cell walls (Marry et al
2005, Jones et al 2005, Bush et al 2001, 1999, Marry et al 2000, Ryden et al 2003) and
growth mechanisms (Derbyshire et al 2007a,b, McCann et al 2007, Steele et al 1997,
McCann et al 1994)
 Characterization of a topoisomerase involved in endoreduplication in plants (SugimotoShirasu et al 2005, 2002)
 Identifying genes involved in early commitment of cells to forming xylem (waterconducting) cells (Mourelatou et al 2004, Milioni et al 2002) and in building secondary
walls of xylem (Dahiya et al 2005a,b, Milioni et al 2001, Domingo et al 1998, Stacey et
al 1995)
 A database contributing spectroscopic data on over 900 homozygous mutant lines of
Arabidopsis for the plant research community (http://cellwall.genomics.purdue.edu,
McCann et al. 2010 submitted, Yong et al 2005).
 Functional analysis of the MYST gene family of rhamnogalacturonan lyases and its role in
plant growth (Zhou et al. 2010 submitted, Disclosure: Improve root system
architecture in plants).
 Annotation of cell wall-related gene families in Arabidopsis, rice, maize, sorghum and
switchgrass (http://cellwall.genomics.purdue.edu, McCann et al. 2010 submitted,
Schnable et al. 2009, Paterson et al. 2009, Penning et al. 2009, Tobias et al. 2008).
 Phenotypic characterization of cell wall mutants and transgenic lines in collaborative work
(Arsovski et al 2009, Daras et al 2009, Dean et al 2007, Sindhu et al 2007, Zografidis et
al 2007, Mackinnon et al 2006, Fraysse et al 2005, Oomen et al 2004, Ha et al 2002,
Pagant et al 2002, Skjot et al 2002, Schindelman et al 2001, Fagard et al 2000, Sorenson
et al 2000).
Publications (refereed journal articles and book chapters):
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1. McCann, M.C., Wells, B. and Roberts, K. (1990) Direct visualisation of cross-links in the
primary plant cell wall. J. Cell Sci. 96, 323-34.
2. McCann, M.C. and Roberts, K. (1991) Architecture of the primary cell wall. In: The
Cytoskeletal Basis of Plant Growth and Form (Ed. C.W Lloyd), Academic Press, New York,
pp 109-129.
3. McCann, M.C., Wells, B. and Roberts, K. (1992) Complexity in the spatial localization and
length distribution of plant cell-wall matrix polysaccharides. J. Micros. 166, 123-136.
4. McCann, M.C., Hammouri, M.K., Wilson, R.H., Belton, P.S. and Roberts, K. (1992). Fourier
transform infra-red microspectroscopy is a new way to look at plant cell walls. Plant Physiol. 100,
1940-1947.
5. Baldwin, T.C., McCann, M.C. and Roberts, K. (1993) A novel hydroxyproline-deficient
arabinogalactan protein secreted by suspension-cultured cells of Daucus carota. Plant Physiol. 103,
115-123.
6. McCann, M.C., Stacey, N.J., Wilson, R. and Roberts, K. (1993) Orientation of macromolecules
in the walls of elongating carrot cells. J. Cell Sci. 106, 1347-1356.
7. Wells, B., McCann, M.C., Shedletzky, E., Delmer, D. and Roberts, K. (1994) Structural features
of cell walls from tomato cells adapted to grow on the herbicide 2,6-dichlorobenzonitrile. J.
Micros. 173, 155-164.
8. McCann, M.C. and Roberts, K. (1994) Plant cell walls: murals and mosaics. Agro-Industry HiTech 2, 43-46.
9. McCann, M.C., Shi, J., Roberts, K. and Carpita, N.C. (1994) Changes in pectin structure and
localisation during the growth of unadapted and NaCl-adapted tobacco cells. Plant J. 5, 773-785.
10. Kemsley, E.K., Belton, P.S., McCann, M.C., Ttofis, S., Wilson, R.H. and Delgadillo, I. (1994) A
rapid method for the authentication of vegetable matter using Fourier transform infrared
spectroscopy. Food Control 5, 241-243.
11. McCann, M.C. and Roberts, K. (1994).
elongation. J. Exp. Bot. 45, 1683-1691.
Changes in cell wall architecture during cell
12. Séné, C.F.B., McCann, M.C., Wilson, R.H. and Grinter, R. (1994) FT-Raman and FT-infrared
spectroscopy: an investigation of five higher plant cell walls and their components. Plant Physiol.
106, 1623-1633.
13. McCann, M.C., Roberts, K., Wilson, R.H., Gidley, M.J., Gibeaut, D.M., Kim, J-B. and Carpita,
N.C. (1995) Old and new ways to probe plant cell-wall architecture. Can. J. Bot. 73 (Suppl.),
S103-S113.
14. Stacey, N.J., Roberts, K., Carpita, N.C., Wells, B. and McCann, M.C. (1995) Dynamic changes
in cell surface molecules are very early events in the differentiation of mesophyll cells from
Zinnia elegans into tracheary elements. Plant J. 8, 891-906.
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15. Belton, P.S., Kemsley, E.K., McCann, M.C., Ttofis, S., Wilson, R.H. and Delgadillo, I. (1995)
The identification of vegetable matter using Fourier transform infrared spectroscopy. Food
Chem. 54, 437-441.
16. Foster, T.J., Ablett, S., McCann, M.C. and Gidley, M.J. (1996) Mobility-resolved
spectroscopy of primary plant cell walls. Biopolymers 39, 51-66.
13C-NMR
17. McCann, M.C. and Roberts, K. (1996) The role of pectin in cell wall architecture. In:
Proceedings of the Symposium on Pectins and Pectinases. Elsevier, Amsterdam, pp 91-107.
18. Carpita, N.C., McCann, M.C. and Griffing, L.R. (1996) The plant extracellular matrix: News
from the cell's frontier. Plant Cell 8, 1451-1463.
19. Carpita, N.C. and McCann, M.C. (1997) Some new methods to study plant polyuronic acids
and their esters. In: Techniques in Glycobiology (Eds. R.R. Townsend, et al.), Marcel Dekker Inc.,
New York, pp595-612.
20. Steele, N.M., McCann, M.C. and Roberts, K. (1997) Pectin modification in cell walls of
ripening tomatoes occurs in distinct domains. Plant Physiol. 114, 373-381.
21. McCann, M.C. (1997) Tracheary element formation: Building up to a dead end. Trends
Plant Sci. 2, 333-338.
22. McCann, M.C., Chen, L., Roberts, K., Kemsley, E.K., Sene, C., Carpita, N.C., Stacey, N.J. and
Wilson, R.H. (1997) Infrared microspectroscopy: sampling heterogeneity in plant cell wall
composition and architecture. Physiol. Plant. 100, 729-738.
23. Ambler, S.J., McCann, M.C., Stacey, N.J., Calder, G., Wells, B. and Roberts, K. (1997)
Measuring cell-wall strength of suspension-cultured carrot cells. In: Plant Biomechanics 1997,
(Eds. G. Jeronimidis & J.F.V. Vincent), pp 151-153.
24. Chen, L., Wilson, R.H. and McCann, M.C. (1997) Infrared microspectroscopy of hydrated
biological systems: design and construction of a new cell with atmospheric control for the study
of plant cell walls. J. Microscopy 188, 62-71.
25. Chen, L., Wilson, R.H. and McCann, M.C. (1997) Investigation of macromolecule orientation
in dry and hydrated walls of single onion epidermal cells by FTIR microspectroscopy. J. Molec.
Struct. 408, 257-260.
26. Domingo, C., Roberts, K., Stacey, N.J., Connerton, I., Ruíz-Teran, F. and McCann, M.C.
(1998) A pectate lyase from Zinnia elegans is auxin inducible. Plant J. 13, 17-28.
27. Chen, L., Carpita, N.C., Reiter, W-D., Wilson, R.H., Jeffries, C. and McCann, M.C. (1998) A
rapid method to screen for cell-wall mutants using discriminant analysis of Fourier transform
infrared spectra. Plant J. 16, 385-392.
28. Bush, M.S. and McCann, M.C. (1999) Pectic epitopes are differentially distributed in the cell
walls of potato (Solanum tuberosum) tubers. Physiol. Plant. 107, 201-213.
29. Carpita, N.C. and McCann, M.C. (2000) Chapter 2: The cell wall. In: Biochemistry and
Molecular Biology of Plants (Eds. B.B. Buchanan, W. Gruissem, R. Jones), American Society Plant
Physiologists, Rockville, MD, pp 52-109.
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30. McCann, M.C., Stacey, N.J. and Roberts, K. (2000) Chapter 14: Targetted cell death in
xylogenesis. In: Cell Death in Health and Disease (Eds. J. Bryant, J.M. Garland, S.G. Hughes),
BIOS Scientific Publishers Ltd., Oxford, pp193-202.
31. McCann, M.C., C. Domingo, Stacey, N.J., Milioni, D. and Roberts, K. (2000) Chapter 37:
Tracheary element formation in an in vitro system. In: Cambium: The Biology of Wood Formation
(Eds. R. Savidge, J. Barnett, R. Napier), BIOS Scientific Publishers Ltd., Oxford, UK, pp 457-470.
32. Marry, M., McCann, M.C., Kolpak, F., Stacey, N.J. and Roberts, K. (2000) Extraction of pectic
polysaccharides from sugar-beet cell walls. J. Food Sci. Agric. 80, 17-28.
33. Karlsson, M., Johansson, I., Bush, M., McCann, M.C., Larsson, C. and Kjellbom, P. (2000) A
spinach TIP expressed in a temporally and spatially distinct pattern. Plant J. 21, 83-90.
34. Vincken, J-P., Borkhardt, B., Bush, M., Doeswijk-Voragen, C., Dopico, B., Labrador, E., Lange,
L., McCann, M.C., Morvan, C., Munoz, F., Oomen, R., Peugnet, I., Rudolph, B., Schols, H.,
Sorenson, S., Ulvskov, P., Voragen, A. and Visser, R. (2000) Remodelling pectin structure in
potatoes. In: Phytosfere ‘99 European Plant Biotechnology Network. (Eds. G.E. de Vries and K.
Metzlaff), Elsevier Science B.V., Dordrecht, The Netherlands, pp 23-34.
35. Jarvis, M.C. and McCann, M.C. (2000) Macromolecular biophysics of the plant cell wall:
concepts and methodology. Plant Physiol. Biochem. 38, 1-13.
36. Sorenson, S.O., Pauly, M., Bush, M.S., Skjot, M., McCann, M.C., Borkhardt, B. and Ulvskov,
P. (2000) Modification of potato pectin by in vivo expression of an endo-1,4-b-D-galactanase.
Proc. Natl. Acad. Sci. U. S. A. 97, 7639-7644.
37. Roberts, K. and McCann, M.C. (2000) Xylogenesis: The birth of a corpse. Curr. Opin. Plant
Biol. 3, 517-522.
38. Fagard, M., Desnos, T., Desprez, T., Goubet, F., Refregier, G., McCann, M.C., Rayon, C.,
Vernhettes, S. and Höfte, H. (2000) Procuste1 encodes a cellulose synthase required for normal
cell elongation specifically in roots and dark-grown hypocotyls of Arabidopsis. Plant Cell 12,
2409-2423.
39. Schindelman, G., Morikami, A., Jung, J., Baskin, T.I., Carpita, N.C., Derbyshire, P., McCann,
M.C. and Benfey P.N. (2001) COBRA encodes a putative GPI-anchored protein, which is
polarly localized and necessary for oriented cell expansion in Arabidopsis. Gen. Dev. 15, 11151127.
40. McCann, M.C., Carpita, N.C. and Roberts, K. (2001) Plant cell growth and elongation. In:
Encyclopedia of Life Sciences. Macmillan Reference Ltd., London.
41. Bush, M., Marry, M., Huxham, I.M., Jarvis, M.C. and McCann M.C. (2001) Developmental
regulation of pectic epitopes during potato tuberisation. Planta 213, 869-880.
42. McCann, M.C., Bush, M., Milioni, D., Sado, P., Stacey, N.J., Catchpole, G., Defernez, M.,
Carpita, N.C., Hofte, H., Wilson, R.H. and Roberts, K. (2001). Approaches to understanding the
functional architecture of the plant cell wall. Phytochemistry 57, 811-821.
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43. Milioni, D., Sado, P-E., Stacey, N.J., Roberts, K. and McCann, M.C. (2001) Differential
expression of cell-wall-related genes during formation of tracheary elements in the Zinnia
mesophyll cell system. Plant Molecular Biology 47, 221-238.
44. Carpita, N.C., Defernez, M., Findlay, K., Wells, B., Shoue, D.A., Catchpole, G.C., Wilson,
R.H., and McCann, M.C. (2001) Cell wall architecture of the elongating maize coleoptile. Plant
Physiol. 127, 551-565.
45. McCann, M.C., Stacey, N.J., Dahiya, P., Milioni, D., Sado, P. and Roberts, K. (2001) Zinnia:
Everybody needs good neighbors. Plant Physiol.127, 1380-1382.
46. Oomen, R.J.F.J., Vincken, J.-P., Bush, M.S., Doeswijk-Voragen, C.H.L., Borkhardt, B., Broek
van den, L.A.M., Ulvskov, P., Voragen, A.G.J., McCann, M.C. and Visser, R.G.F. (2002) Towards
unravelling the biological significance of the individual components of pectic hairy regions in
plants. In: Conference proceedings of Pectins and Pectinases 2001 (Visser, R.G.F., Schols, H.A.
and Voragen, A.G.J., eds) Amsterdam: Elsevier Science B.V.
47. Skjot, M., Pauly, M., Bush, M.S., Borkhardt, B., McCann, M.C. and Ulvskov, P. (2002) Direct
interference with pectin biosynthesis in Golgi vesicles. Plant Physiol 129, 95-102.
48. Carpita, N.C. and McCann, M.C. (2002) The functions of cell wall polysaccharides in
composition and architecture revealed through mutations. Plant and Soil Science 247, 71-80.
49. Oomen, R.J.F.J., Doeswijk-Voragen, C.H.L., Bush, M.S., Vincken, J-P., Borkhardt, B., van den
Broek, L.A.M., Corsar, J., Ulvskov, P., Voragen, A.G.J., McCann, M.C. and Visser, R.G.F. (2001)
In muro fragmentation of the rhamnogalacturonan I backbone in potato (Solanum tuberosum L.)
results in a reduction and altered location of the galactan and arabinan side-chains and
abnormal periderm development. The Plant Journal 30, 403-413.
50. Milioni, D., Sado, P., Stacey, N.J., Roberts, K., and McCann, M.C. (2002) Early gene
expression associated with the commitment and differentiation of a plant tracheary element is
revealed by cDNA-Amplified Fragment Length Polymorphism analysis. The Plant Cell 14, 28132824.
51. Pagant, S., Bichet, A., Sugimoto, K., Lerouxel, O., Desprez, T., McCann, M.C., Lerouge, P.,
Vernhettes, S. and Hofte, H. (2002) KOBITO1 encodes a novel plasma membrane protein
necessary for normal synthesis of cellulose during cell expansion in Arabidopsis. The Plant Cell 14,
2001-2013.
52. Vincken, J-P., Schols, H.A., Oomen, R.J.F., McCann, M.C., Ulvskov, P., Voragen, A.G.J. and
Visser, R.G.F. (2002) If homogalacturonan were a side chain of rhamnogalacturonan I.
Implications for cell wall architecture. Plant Physiology 132, 1781-1789.
53. Ha, M.A., Mackinnon, I. M., Sturcova, A., Apperley, D.C., McCann, M.C., Turner, S.R. and
Jarvis, M.C. (2002) Structure of cellulose-deficient secondary cell walls from the irx3 mutant of
Arabidopsis thaliana. Phytochemistry 61, 7-14.
54. Sugimoto-Shirasu, K., Stacey, N.J., Corsar, J., Roberts, K. and McCann, M.C. (2002) DNA
topoisomerase VI is essential for endoreduplication in Arabidopsis. Current Biology 12, 1782-1786.
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55. Ryden, P., Sugimoto-Shirasu, K., Smith, A.C., Findlay, K., Reiter, W-D. and McCann, M.C.
(2003) Tensile properties of Arabidopsis cell walls depend on both a xyloglucan cross-linked
microfibrillar network and rhamnogalacturonan II-borate complexes. Plant Physiology 132, 10331040.
56. Sugimoto-Shirasu, K., Carpita, N.C. and McCann, M.C. (2003) The cell wall: a sensory panel
for signal transduction. In: The plant cytoskeleton in cell differentiation and development. (P.
Hussey, Ed.), Blackwell Publishers, UK, Chapter 6, pp176-203.
57. Oomen, R.J.F.J., Tzitzikas, E.N., Bakx, E.J., Straatman-Engelen, I., Bush, M.S., McCann, M.C.,
Schols, H.A. Visser, R.G.F. and Vincken, J-P. (2004) Modulation of the cellulose content of tuber
cell walls by antisense expression of different potato (Solanum tuberosum L.) CesA clones.
Phytochemistry 65, 535-546.
58. Mourelatou, M., Doonan, J.H. and McCann, M.C. (2004) Transition of G1 to early S phase
may be required for Zinnia mesophyll cells to trans-differentiate to tracheary elements. Planta
220, 172-176.
59. Kemsley, E.K., Swinhoe, R.R., Smith, A.C., McCann, M.C. and Wang, T.L. (2004)
Chemometrics can be applied to mechanical testing data to characterise stem toughness and
stiffness in crop plants. J Sci Food Agric 84, 966-976.
60. Yong, W., Link, B., O’Malley, R., Tewari, J., Lu, C., Li, X., Bleecker, A.B., Koch, K.E., McCann,
M.C., McCarty, D.R., Patterson, S.E., Reiter, W-D., Staiger, C., Thomas, S., Vermerris, W. and
Carpita, N.C. (2005) Genomics of plant cell wall biogenesis. Planta 221, 747-751.
61. Jones, L., Milne, J.L., Ashford, D., McCann, M.C. and McQueen-Mason, S.J. (2005) A
conserved functional role of pectic polymers in stomatal guard cells from a range of plant
species. Planta 221, 255-264.
62. McCann, M.C. and Carpita, N.C. (2005) Screening for cell-wall phenotypes by infrared
spectroscopy. In: Hayashi, T., ed., Frontiers in Plant Cell Walls, Elsevier, Dordrecht, pp. 63-70.
63. McCann, M.C. (2005) Secondary wall formation in an in vitro system. In: Hayashi, T., ed.,
Frontiers in Plant Cell Walls, Elsevier, Dordrecht, pp. 54-62.
64. McCann, M.C. and Carpita, N.C. (2005) Looking for invisible phenotypes in cell wall mutants
of Arabidopsis thaliana. Plant Biosystems 139, 80-83.
65. Dahiya, P., Milioni, D., Wells, B., Stacey, N., Roberts, K. and McCann M.C. (2005) A RINGdomain gene is expressed in different cell types of leaf-trace, stem and juvenile bundles in the
stem vascular system of Zinnia elegans cv Envy. Plant Physiology 138, 1383-1395.
66. de Vries, R.P., McCann, M. and Visser, J. (2005) Modification of plant cell wall
polysaccharides using enzymes from Aspergillus. In: K.J. Yarema (ed.) Handbook of
Carbohydrate engineering, CRC Press, Taylor and Francis Group, Boca Raton, FL, pp 613-644.
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67. Marry, M., Roberts, K., Jopson, S.J., Huxham, I.M., Jarvis, M.C., Corsar, J., Robertson, E. and
McCann, M.C. (2005) Cell-cell adhesion in sugar-beet parenchyma requires both pectin esters
and calcium cross-links. Physiologia Plantarum 126, 243-256.
68. Sugimoto-Shirasu, K., Roberts, G.R., Stacey, N.J., McCann, M.C., Maxwell, A. and Roberts, K.
(2005) RHL1 is an essential component of the plant DNA topoisomerase VI complex and is
required for ploidy-dependent cell growth. Proc Natl Acad Sci USA 102, 18736-18741.
69. Fraysse, L., Wells, B., McCann, M.C. and Kjellbom, P. (2005) Specific plasma membrane
aquaporins of the PIP1 subfamily are expressed in sieve elements and guard cells. Biology of the
Cell 97, 519-534.
70. Dahiya, P., Findlay, K., Roberts, K. and McCann M.C. (2006) A fasciclin-domain containing
gene, ZeFLA11, is expressed exclusively in xylem elements that have reticulate wall thickenings
in the stem vascular system of Zinnia elegans cv Envy. Planta 223, 1281-1291.
71. Mackinnon, I.M., Sturcova, A., Sugimoto-Shirasu, K., His, I., McCann, M.C. and Jarvis, M.C.
(2006) Cell-wall structure and anisotropy in procuste, a cellulose synthase mutant of Arabidopsis
thaliana. Planta 224, 438-448.
72. McCann, M.C., Defernez, M., Urbanowicz, B.R., Tewari, J.C., Langewisch, T., Olek, A., Wells,
B., Wilson, R.H. and Carpita, N.C. (2007) Neural network analyses of infrared spectra for
classifying cell wall architectures. Plant Physiology 143, 1314-1326.
73. Derbyshire, P., Findlay, K., McCann, M.C. and Roberts, K. (2007) Cell elongation in
Arabidopsis hypocotyls involves dynamic changes in cell wall thickness. J Experimental Botany 58,
2079-2089.
74. Derbyshire, P., McCann, M.C. and Roberts, K. (2007) Restricted cell elongation in
Arabidopsis hypocotyls is associated with a reduced average pectin esterification level. BioMed
Central (BMC) Plant Biology 7, 31.
75. Sindhu, A., Langewisch, T., Olek, A., Multani, D.S., McCann, M.C., Vermerris, W.R., Carpita,
N.C. and Johal, G. (2007) Maize Brittle Stalk2 encodes a COBRA-like protein expressed in early
organ development but requited for tissue flexibility at maturity. Plant Physiology 145, 14441460.
76. Zografidis, A., Kapolas, G., Kitsios, G., McCann, M.C., Roberts, K., Milioni, D. and
Haralampidis, K. (2007) Isolation and characterization of ZePES and AtPES, the pescadillo
orthologs from Zinnia and Arabidopsis. Plant Science 173, 358-368.
77. Dean, G.H., Zheng, H., Tewari, J.C., Young, D.S., Hwang, Y-T., Western, T., Carpita, N.C.,
McCann, M.C., Mansfield, S.D. and Haughn, G.W. (2007) The Arabidopsis MUM2 gene encodes
a beta-galactosidase required for the production of seed coat mucilage with correct hydration
properties. Plant Cell 19, 4007-4022.
78. McCann, M.C. and Carpita, N.C. (2008) Designing the deconstruction of plant cell walls.
Current Opinion in Plant Biology 11, 314-321.
10
79. McCann, M.C. and Carpita, N.C. (2008) Maize and sorghum: genetic resources for bioenergy
grasses. Trends in Plant Sciences 13, 415-421.
80. Tobias, C.M., Sarath, G., Twigg, P., Lindquist, E., Pangilinan, J., Penning, B.W., Barry, K.,
McCann, M.C., Carpita, N.C. and Lazo, G.R. (2008) Comparative genomics in switchgrass using
61,585 high-quality expressed sequence tags. The Plant Genome 1, 112-124.
81. Paterson, A.H., Bowers, J.E., Bruggmann, R., Dubchak, I., Grimwood, J., Gundlach, H.,
Haberer, G., Hellsten, U., Mitros, T., Poliakov, A., Schmutz, J., Spannagl, M., Tang, H., Wang, X.,
Wicker, T., Bharti, A.K., Chapman, J., Feltus, F.A., Gowik, U., Grigoriev, I.V., Lyons, E., Maher,
C.A., Martis, M., Narechania, A., Otillar, R.P., Penning, B.W., Salamov, A.A., Wang, Y., Zhang,
L., Carpita, N.C., Freeling, M., Gingle, A.R., Hash, C.T., Keller, B., Klein, P., Kresovich, S.,
McCann, M.C., Ming, R., Peterson, D.G., Mehboob-ur-Rahman, Ware, D., Westhoff, P., Mayer,
K.F.X., Messing, J. and Rokhsar, D.S. (2009) The Sorghum bicolor genome and the diversification
of grasses. Nature 457, 551-557.
82. Schnable, P.S. Ware, D., Fulton, R.S., Stein, J.C., Wei, F., Pasternak, S., Liang, C., Zhang, J.,
Fulton, L. Graves, T.A., Minx, P., Reily, A.D., Courtney, L., Kruchowski, S.S., Tomlinson, C.,
Strong, C., Kim Delehaunty, K., Fronick, C., Courtney, B., Rock, S.M., Belter, E., Du, F., Kim, K.,
Abbott, R.M., Cotton, M., Levy, A., Marchetto, P., Ochoa, K., Jackson, S.M., Gillam, B., Chen, W.,
Yan, L., Higginbotham, J., Cardenas, M., Waligorski, J., Applebaum, E., Phelps, L., Falcone, J.,
Kanchi, K., Thane, T., Scimone, A., Thane, N., Henke, J., Wang, T., Ruppert, J., Shah, N., Rotter,
K., Hodges, J., Ingenthron, E., Cordes, M., Kohlberg, S., Sgro, J., Delgado, B., Mead, K.,
Chinwalla, A., Leonard, S., Crouse, K., Collura, K., Kudrna, D., Currie, C., He, R., Angelova, A.,
Rajasekar, S., Mueller, T., Lomeli, R., Scara, G., Ko, A., Delaney, K., Wissotski, M., Lopez, G.,
Campos, D., Braidotti, M., Ashley, A., Golser, W., Kim, H.-R., Lee, S., Lin, J., Dujmic, Z., Kim,
W., Talag, J., Zuccolo, A., Fan, C., Sebastian, A., Kramer, M., Spiegel, L., Nascimento, L.,
Zutavern, T., Miller, B., Ambroise, C., Muller, S., Spooner, W., Narechania, A., Ren, L., Wei, S.,
Kumari, S., Faga, B., Levy, M.J., McMahan, L., Peter Van Buren, P., Vaughn, M.W., Ying, K., Yeh,
C-T., Emrich, S.J., Jia, Y., Kalyanaraman, A., Hsia, A.-P., W. Barbazuk, W.B., Baucom, R.S.,
Brutnell, T.P., Carpita, N.C., Chaparro, C., Chia, J.-M., Deragon, J.-M., Estill, J.C., Fu, Y.,
Jeddeloh, J.A., Han,Y., Lee, H., Li, P., Lisch, D.R., Liu, S., Liu, Z., Nagel, D.H., McCann, M.C.,
SanMiguel, P., Myers, A.M., Nettleton, D., Nguyen, J., Penning, B.W., Ponnala, L., Schneider,
K.L., Schwartz, D.C., Sharma, A., Soderlund, C., Springer, N.M., Sun, Q., Wang, H., Waterman,
M., Westerman, R., Wolfgruber, T.K., Yang, L., Yu,Y., Zhang, L., Zhou, S., Zhu, Q., Bennetzen,
J.L., Dawe, R.K., Jiang, J., Jiang, N., Presting, G.G., Wessler, S.R., Aluru, S., Martienssen, R.A.,
Clifton, S.W., McCombie, W.R., Wing, R.A., Wilson, R.K. (2009) The B73 maize genome:
Complexity, diversity, and dynamics. Science 326, 1112-1115.
83. Arsovski, A.A., T.M. Popma, G.W. Haughn, N.C. Carpita, M.C. McCann, T. L. Western,
(2009) AtBXL1 encodes a bifunctional b-D-xylosidase/a-L-arabinofuranosidase required for
pectic arabinan modification in Arabidopsis mucilage secretory cells. Plant Physiol. 150, 12191234.
84. Daras, G., S. Rigas, B. Penning, D. Milioni, M.C. McCann, N. C. Carpita, Fasseas, C., P.
Hatzopoulos (2009) The thanatos mutation in Arabidopsis cellulose synthase 3 (AtCesA3) has a
semidominant-negative phenotype for cellulose synthesis and plant growth. New Phytol.184,
114-126.
11
85. Penning, B., Hunter, C.T., Tayengwa, R., Eveland, E., *Dugard, C.K., Olek, A. Vermerris, W.,
Koch, K.E., McCarty, D.R., Davis, M., Thomas, S.R., McCann, M.C., Carpita, N.C. (2009) Genetic
resources for maize cell wall biology. Plant Physiol. 151, 1703-1728.
86. Carpita, N.C. and McCann, M.C. (2010) The maize mixed-linkage (1,3), (1,4)-beta-D-glucan is
synthesized at the Golgi membrane. Plant Physiol., in press.
87. McCann, M.C. and Knox, J.P. (2010) Plant cell wall biology: polysaccharides in architectural
and developmental contexts. In Plant Polysaccharides, Ed. P Ulvskov, Blackwell Publishing,
Oxford UK, in press.
88. McCann, M.C. and Rose, J. C. (2010) Editorial: Cell Walls. Focus Issue on Plant Cell Walls.
Plant Physiol., 153, 365.
89. White, W.G., Moose, S.P., Weil, C.F., McCann, M.C., Carpita, N.C. and Below F.E. (2010)
Tropical Maize – Exploiting maize genetic diversity to develop a novel annual crop for
lignocellulosic biomass and sugar production. In Springer, in press.
90. Carpita, N.C., Ralph, J. and M.C. McCann. (2010) Chapter 2: The cell wall. In: Biochemistry
and Molecular Biology of Plants (Eds. B.B. Buchanan, W. Gruissem, R. Jones), American Society
Plant Physiologists, Rockville, MD, invited chapter for 2nd edition, in press.
Invited talks:
Royal Microscopical Society: 4th International Botanical Microscopy Meeting, Durham UK
(1991)
Cellulose ‘91, New Orleans (1991)
Keystone meeting, The plant extracellular matrix, Santa Fe (1993)
Cytonet meeting, Breckenridge, Colorado (1993)
Plenary: Society for Experimental Biology, Swansea UK (1994)
Plant Biomechanics Meeting, Montpellier, France (1994)
Plenary: Royal Microscopical Society: 5th International Botanical Microscopy meeting, Oxford
UK (1995)
Cytonet meeting, Kona, Hawaii (1995)
Scottish Cell Wall Meeting, Edinburgh UK (1995)
International Society for Plant Growth Regulators meeting, Minneapolis (1995)
7th International Cell Wall Meeting, Santiago de la Compostela, Spain (1995)
Keynote (Pilnik lecture): Pectins and pectinases, Wageningen, The Netherlands (1995)
Plenary: Hercules Analytical Symposium, Wilmington, Delaware USA (1996)
Australian Society of Plant Physiology and Australian Society of Plant Molecular Biology,
Canberra, Australia (1996)
Plenary: Swedish Society of Plant Physiology, Uppsala, Sweden (1997)
Cytonet Meeting, Breckenridge, Colorado (1997)
Gordon Conference – Cell walls, Tilton, New Hampshire (1997)
Plenary: Society for Experimental Botany Satellite meeting, Leeds, UK (1998)
Keynote: Opening of New Centre for Molecular Plant Physiology, Copenhagen (1998)
Plenary: Brazilian Society for Plant Biochemistry and Molecular Biology, Caxambu, Brazil (1998)
International Association of Plant Tissue Culture, Jerusalem (1998)
8th International Cell Wall Meeting, Norwich, UK (1998)
Society of Experimental Botany: Programmed cell death in health and disease, Exeter, UK (1998)
Society of Experimental Botany: Cambium - the biology of wood formation, Edinburgh, UK
(1999)
12
COST meeting (invited expert): Gametic morphogenesis in monocots. Jokkionnen, Finland
(1999)
International Botanical Congress, St Louis, Missouri (1999)
John Innes Centre Annual Science meeting, UK (1999)
Juan March meeting, The plant extracellular matrix, Madrid, Spain (1999)
American Chemical Society meeting, San Francisco (2000)
Society for In Vitro Biology meeting, San Diego (2000)
Gordon Research Conference – Cell walls, New Hampshire (2000)
Banbury meeting – Application of Arabidopsis genomics to forestry, Cold Spring Harbor
Laboratory (2000)
Plenary: Wood Biotechnology, Narita, Japan (2001)
Plant Protein Club – Cell walls, York, UK (2001)
American Chemical Society meeting, San Diego (2001)
Plenary: COST meeting (invited expert) – Wood properties. Uppsala, Sweden (2001)
Plenary: 4th Carbohydrate Bioengineering meeting, Stockholm, Sweden (2001)
Plenary: American Society for Space and Gravitational Biology, Washington DC (2001)
Developmental Biology Workshop, John Innes Centre (2002)
EMBO course “Plant Cell Biology”, Wageningen, The Netherlands (2002)
American Society for Plant Biology, Denver (2002)
12th European Carbohydrate Symposium, Grenoble, France (2003)
American Society for Plant Biology, Honolulu, Hawaii (2003)
Gordon Research Conference – Cell walls, New Hampshire (2003)
Pennsylvania State University Plant Physiology Symposium (2004)
10th International Cell Wall meeting, Sorrento, Italy (2004)
Plant Center Retreat Symposium, University of Georgia (2004)
Illinois Microscopical Society, Chicago (2005)
American Society for Plant Biology, Seattle (2005)
Cell Wall Biosynthesis Meeting, Asilomar, California (2005)
EPOBIO Workshop, Products from Plants, Wageningen, The Netherlands (2006)
Gordon Research Conference – Plant cell walls, New Hampshire (2006)
International Society for Plant Molecular Biology, Adelaide, Australia (2006)
TAPPI Nanotechnology for the forest products industry meeting, Knoxville, Tennessee (2007)
American Society for Plant Biology, Chicago (2007)
11th International Cell Wall meeting, Copenhagen, Denmark (2007)
Pan-American Congress in Plants and Bioenergy, Merida, Mexico (2008)
Microscopy and Microanalysis, Albuquerque, New Mexico (2008)
48th Annual MidWest Developmental Biology meeting, Iowa City, Iowa (2009)
American Society for Plant Biology, Honolulu, Hawaii (2009)
Chemical Engineering of the Future Symposium, Purdue University, USA (2009)
XIII National and VI Mexico-US Congress of Plant Molecular Biology, Guanajuato (2009)
DOE-EERE Biomass 2010 Conference Washington DC (2010)
12th International Cell Wall meeting, Porto, Portugal (2010)
2nd Pan-American Congress in Plants and Bioenergy, Pericicaba, Brazil (2010)
Invited seminars:
University of Oxford, Zoology Department, Oxford UK, (1992)
Purdue University, Department of Botany and Plant Pathology, Indiana USA, (1994)
Institut für Pflanzenbiologie, Department of Botany, Zurich, (1994)
University of East Anglia, Department of Biological Sciences, Norwich UK, (1996)
University of Cambridge, Department of Plant Sciences, Cambridge UK, (1996)
Department of Molecular Genetics, John Innes Centre, Norwich UK, (1996)
University of Melbourne, Australia, (1996)
13
University of Lund, Sweden, (1996)
University of York, UK, (1997)
Complex Carbohydrate Research Center, University of Georgia, USA, (1997)
University of Madison, Wisconsin USA, (1997)
Purdue University, Department of Botany and Plant Pathology, Indiana USA, (1997)
University of Campinas, Department of Biology, Brazil, (1998)
University of São Paulo, Department of Food Science, Brazil, (1998)
University of São Paulo, Department of Botany and Biology, Brazil, (1998)
INRA, Versailles, France, (1998)
University of Lausanne, Department of Plant Biology, Switzerland, (1998)
University of Oxford, Department of Plant Sciences, UK, (2000)
Purdue University, Department of Botany and Plant Pathology, Indiana USA, (2000)
University of Heidelberg, Botanischer Institut , Germany (2001)
Department of Brassicas and Oilseed Research, John Innes Centre, UK, (2001)
Department of Cell and Developmental Biology, John Innes Centre, UK, (2001)
Department of Forestry, University of Umea, Sweden (2002)
Max Planck Institut, Golm, Germany (2002)
Ohio University, USA (2003)
Pennsylvania State University, USA (2003)
Oklahoma State University, USA (2004)
Kansas State University, USA (2004)
DOE-Plant Research Laboratory, Michigan State University, USA (2005)
USDA-ARS Laboratory, UC-Berkeley, Albany, USA (2005)
Carnegie Institution, Stanford University, USA (2006)
Brookhaven National Laboratory (2006)
University of Illinois, Urbana-Champaign, USA (2006)
University of Lausanne, Switzerland (2006)
University of California-Davis, USA (2006)
Ohio State University, USA (2006)
University of Tennessee, USA (2008)
Oak Ridge National Laboratory, USA (2008)
IUPUI, Indianapolis, USA (2009)
Indiana Life Sciences Forum, Indianapolis, USA (2009)
Indiana Society of American Foresters Retreat, USA (2010)
Pennsylvania State University, USA (2010)
Grants held (current in bold):
DOE
Center for the Direct Catalytic Conversion of
Biomass to Biofuels
(+ 24 Co-Investigators)
NSF
Maximizing conversion of biomass carbon to
liquid fuel (+ 5 Co-Investigators)
Function of rhamnogalacturonan-I in tomato
USDA
cell walls (with A Handa)
Partnership for Research and Education in
USDA
plant breeding and genetics at Purdue (+ 13
Co-Investigators)
DOE
Translational genomics for the improvement
of switchgrass (with N Carpita)
08/01/2009 07/31/2014
$20,000,000
09/01/2009 08/31/2012
01/01/2010 12/31/2012
01/01/2010 12/31/2013
$2,000,000
09/15/0809/14/11
$1,200,000
$350,000
$497,672
14
NSF-MRI Dual beam scanning electron microscope for
hydrated and soft material applications (+ 5
Co-Investigators)
NSF
A high through-put screen to identify cell-wall
mutants in maize and Arabidopsis
DOE
The role of pectin in cell elongation
09/01/0608/31/10
09/01/0208/31/07
09/01/0308/31/07
$673,000
$5,992,000
$380,000
EU-FIV- Network (1997-2000) Remodelling pectin structure in plants £123K
MAFF (1998-2003) Straw strength of pea stems (joint with T. Wang, John Innes Centre) £270K
BBSRC (1998-2000) Electron Energy Loss Spectroscopy (EELS) for elemental imaging of the
plant cell wall (joint with M. Jarvis, M. Huxham and D. McComb, University of Glasgow) £165K
BBSRC (1998-2000) The role of pectin in plant cell elongation (joint with R. H. Wilson, Institute
of Food Research) £124K
BBSRC (1999-2002) Program grant: Molecular architecture and structural properties of plant cell
walls (joint with K. Roberts, John Innes Centre, and R. H. Wilson, Institute of Food Research)
£511K
BBSRC (2000) Joint Research Equipment Initiative grant £50K plus matching monies from JIC
and IFR, total value £100K.
EU-FV-Network (2000-2003) Genetic determinism in maritime pine £172K
Leverhulme Trust (2001-2002) Cell specialisation in higher plants: a molecular analysis (joint
with K. Roberts, JIC) £130K
BBSRC (2000-2003) The expression and function of matrix metalloproteinase homologues in
Arabidopsis thaliana (joint with I. Clark and G. Murphy, University of East Anglia) £200K
EU-FV-Network (2001-2005) Enzyme discovery in hybrid aspen for fibre engineering £160K
PhD Studentships:
Nancy M Steele, BBSRC/Unilever studentship (1992-1995) Ripening-associated changes in cell
wall architecture of tomatoes (with K. Roberts, JIC).
Christophe Séné, EC/ Flair funded studentship (1992-1995) Infrared and Raman spectroscopy
of cell walls (with R. Grinter, UEA, and R.H. Wilson, IFRN).
Li-mei Chen, Norwich Research Park studentship (1994-1997) Vibrational spectroscopy of plant
cell walls (with J. Davies, UEA, and R.H. Wilson, IFRN).
Juliet Ambler, BBSRC Special studentship (1995-1998) Plant cell-cell adhesion (with K. Roberts,
JIC).
Andrew Marry, Hercules-funded studentship (1995-1998) Characterisation of cell walls and
extracted polysaccharides from Beta vulgaris (with K. Roberts and P. Christou, JIC).
Maria Mourelatou, studentship (1998-2001) Early events in plant cell commitment: choosing
between proliferation and differentiation (with J. Doonan, JIC).
Pierre Sado, BBSRC Special studentship (1998-2001) Secondary wall formation in an in vitro
system.
Paul Derbyshire, BBSRC AFD studentship (1998-2001) The plant cell wall and plant cell
elongation: structural and molecular approaches (with K. Roberts, JIC).
15
Guy Wheeler, BBSRC Case studentship (2000-2003) Homeobox genes in tracheary element
formation (with K. Roberts, JIC).
At Purdue:
Shaohua Zhou (2004-2010) Arabidopsis MYST6 encodes a rhamnogalacturonan lyase that
modulates root growth
Khaldoun Al-Hadid (joint with N Carpita, Department of Botany) (2005-2009) The role of pectin
acetyl esterase in cell elongation
Faisal Chaudhry (2006-2007) Genetic and biochemical analyses of Arabidopsis thaliana glycosyl
hydrolase Family 43 enzymes A and B (AtGH43A and AtGH43B)
Tiffany Langewisch (Masters student) (2004-2006) Cellulose synthesis in maize
Post-doctorals
Concha Domingo
Andrew Marry
Max Bush
Dimitra Milioni
Keiko Sugimoto-Shirasu
Dan Fulton
Preeti Dahiya
Sarah de Vos
1995-1998
1998-2000
1997-2002
1999-2002
2000-2003
2000-2002
2000-2003
2001-2003
At Purdue:
Jagdish Tewari
Kamal Malik
Bryan Penning
Mauricio Antunes
2004-2006
2004-2006
2006-present
2009-present
Service
A. Department
Graduate Admissions and Recruiting, 2003-2006 (Chair, Spr. 06)
Developmental Biology Faculty Search Committee, 2004-2005
Electron Microscope Users Committee, 2004-present
EM Search Committee, 2004-2005
Plant Development Search Committee, 2004-2005
Microbiology – biofuels Search Committee, 2007-2008
Assistant Head of Department, 2007-present
Faculty Council, 2007-present
Chair, Continuing lecturer search committee, 2008
Biofuels search committee, 2008
Chair, Strategic planning committee, 2008-2009
B. College
Strategic Planning Discovery Pillar Group, 2007-2008
College of Science Strategic Plan Oversight Committee, 2009-
16
Associate Dean Search Committee, 2010
C. University
Life Sciences Microscopy Facility advisory committee
Purdue Electron Microscopy Consortium
PULSe Recruitment Committee, 2004-2007
Chair, Plant Biology Training Group, 2007