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CHEMICAL LABORATORY (MALAYSIA) SDN BHD (27822-K) 81-85 (2nd & 3rd Floor), Jalan SS25/2, Taman Bukit Emas, 47301 Petaling Jaya, Selangor Darul Ehsan, Malaysia Tel: 03-7803 2229, 7803 9582, 7803 0386 Fax: 03-7803 2303 ASSAYERS, ANALYTICAL CHEMISTS, MICROBIOLOGISTS AND INDUSTRIAL CONSULTANTS SWAB SAMPLING REPORT For PACKAGING CENTRE SERVICES SDN BHD Lot 6065, Jalan Haji Abd Manan, Batu 5 ½ Meru, 41050 Klang, Selangor. Conducted on the 18th of April 2006 Reported on the 24th of April 2006 Laboratory Number: PJ-K/MS/1599/06 TABLE OF CONTENTS ITEM DESCRIPTION PAGE 1.0 INTRODUCTION 1 2.0 PURPOSE 1 3.0 ANALYSIS PROCEDURE 1 4.0 RESULTS OF ANALYSIS 2 5.0 CONCLUSION 2 APPENDIX -Photographs of the Sampling. Packaging Centre Services Sdn Bhd Swab Sampling Analysis Report Page : 1 of 2 1.0 INTRODUCTION : Swab Sampling and analysis was conducted in a vehicle at the premises of Packaging Centre Services Sdn Bhd, Lot 6065, Jalan Haji Abd Manan, Batu 5 ½, Meru, 41050 Klang, Selangor. 2.0 PURPOSE : The objective of this study is to evaluate the efficiency of the fogging exercise prevailing in the ambient and surface environment within the allocated area. 3.0 ANALYSIS PROCEDURE : The swab sampling for microbial organism was conducted using a sterile swab and sterile distilled water for getting moisture condition. Upon swabbing the surface area selected for sampling, the swab is taken back to the laboratory incubation of test media sampled to promote colonies growth. a) Incubation for Total Bacteria Count using Tryptic Soy Agar (TSA) @ 35oC, 48 hours. b) Incubation for Total Yeast & Mould using Sabauroud Dextrose Agar (SDA) @ 25oC, 5 days. At the end of the incubation cycle, each plate would be observed for any bacterial and fungal growth in the form of colony forming units (cfu). Interpretation of the results are based on the number of colony forming units that were present and counted on the media plates at the end of the incubation cycle. Packaging Centre Services Sdn Bhd Swab Sampling Analysis Report Page : 2 of 2 4.0 RESULTS OF ANALYSIS : Location Total Bacteria Count, cfu/swab Total Mould Count, cfu/swab Total Yeast Count, cfu/swab Driver Side Top Visor (PRE) NG (<10) NG (<10) NG (<10) Driver Side Top Visor (POST) NG (<10) NG (<10) NG (<10) 80 120 NG (<10) NG (<10) NG (<10) NG (<10) Steering Wheel (PRE) NG (<10) 905 NG (<10) Steering Wheel (POST) NG (<10) NG (<10) NG (<10) Test Method In-House Based on USP 28 In-House Based on USP 28 In-House Based on USP 28 Centre Air Conditioner Duct (PRE) Centre Air Conditioner Duct (POST) 5.0 CONCLUSION : Based on the laboratory data as obtained from the validation of microbial count in colony forming units (cfu/swab) when compared with the pre and post fogging results, it was noted that the fogging was successful in lowering the bacterial count in the vehicle concern. VICTOR K.S. CHOONG B.Sc., A.M.I.C. A.O.A.C, MMOSTA General Manager