Download Monoclonal Antibody to Intra-Acrosomal Protein (Canine) Purified

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Transcript
11-281-C100
Monoclonal Antibody to Intra-Acrosomal Protein (Canine)
Purified Antibody (0.1 mg)
Clone:
Ds-2
Isotype:
Mouse IgG
Specificity:
The antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42
kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal
membranes and matrix of the mature sperm.
Regulatory Status:
RUO
Immunogen:
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic
acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against
0.2% acetic acid, lyophylized and subsequently used for immunization.
Species Reactivity:
Canine (Dog)
Application:
Immunocytochemistry
Application note: Membrane permeabilization (acetone) is essential.
Western Blotting
Purity:
> 95% (by SDS-PAGE)
Purification:
Purified from ascites by protein-A affinity chromatography.
Concentration:
1 mg/ml
Storage Buffer:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability:
Store at 2-8oC. Do not freeze. Do not use after expiration date stamped on vial
label.
Expiration:
See vial label
Lot Number:
See vial label
References:
*Geussova G, Peknicova J, Capkova J, Kalab P, Moos J, Philimonenko VV, Hozak
P: Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing
acrosomal status during capacitation and acrosome reaction. Andrologia. 1997
Sep-Oct;29(5):261-8.
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