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89 J Chin Med 13(2): 89-96 , 2002 EFFECTS OF CVT..E002, A PROPRIETARY EXTRACT FROM THE NORTH AMERICAN GINSENG (Panax quínquefoJium) ON HEP ATIC DRUG..l\宜ETABOLIZING ENZYMES IN C57BL/6J MICE Yune-Fang Ueng and Chieh-Fu Chen National Research 1nstitute ofChinese Medicine Taipei, Ttα Iwan (Received 14th January 2002 , revised MS received 7' h Februαry 2002, accepted 7' h Februαry2 00刀 CVT-E002 , a proprietary extract from No的American ginseng (Panax quinquφlium) show吋 irnmunomodulating activity. Cytochrome P450 (CYP), UDP-glucuronosyl transferase (UGD , and glutathione S-transferase (GST) are important drug-metabolizing en可mes . Modulation of drugmetabol 位 ing en勾Imes is a main cause of drug interactions. To assess the possible metabolism-based drug interaction of CVT-E002 , e仔ects of CVT-E002 on hepatic CYP , UGT, and GST were studied in C57B Ll6J mice. Treatrnent of mice with 5g1kglday CVT-E002 for three days had no e能cts on \i ver c戶ochrome bs contents and NADPH-CYP reductase activity. CVT-E002 had no effect on microsomal CYP catalytic activities ofthe oxidations of7 -ethoxyresorufin , 7-methoxyresorufin , microsomal CYP and be阻o(a)pyrene , 7-ethoxycoumarin , benzphetamine , N-ni甘osodimethylamine, erythromycin , and nifedipine in mouse liver. Hepatic microsomal UGT and cytosolic GST activities were not affected by CVT-E002-甘eatrnent. Th ese results suggested that CVT-E002 had no effects on hepatic CYP, UGT, and GST activities in mice under this 甘eatrnent reglmen. 甘lere might be low potential of incidence of metabolism-based drug interaction by CVT-E002 Key words: CVT-E002 , Cytochrome P450 , UDP-Glucuronosyl transferase , Glutathione Stransferase INTRODUCTION Modulation of drug-metabolizing enzymes can change the plasma concentrations of drugs and result in serious drug interactions in humans. Phase 1 and phase 11 drug-metabolizing enzymes play important roles in the determination ofpharmacological and toxicological effects of drugs 1. Cytochrome P450 (CYP)-dependent Cor respondence to: D r. Yune-Fang Ueng , National Research lnstitute ofChinese Medicine , 155-1 , Li-Nong S甘E仗, 2 , Taipei 112 , Taiwan , R. O. C., Tel: 02必201999 Ext. 6351 , F似 : 02-28264266 , E-mail: ueng@cm a2 3.nricm .edu.tw Sec.