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Pulse
Electroporation
Onyx Medical, Inc.
Various TDD Technologies
• Electroporation - Using short electrical modulated pulses of high voltage to create
transient aqueous pores in the skin.
• Iontophoresis – Using low voltage electrical current to drive charged product through the
skin.
• Sonophoresis – Using ultrasound to disrupt the stratum corneum.
• Thermal energy – Using heat to make the skin more permeable
• Magnetophoresis - has been investigated as a means to increase assimilation across the
skin.
• Needle Free Injection - devices for “firing” molecules through the skin at high velocity
Electroporation
• Non-invasive
• Alternative to mesotherapy
• Virtual mesotherapy or without needles
• Painless procedure
• Introduction of drugs
• Alternating current
• Acts as a virtual syringe
• Changes direction (polarity) continuously
slowly
• Generating new pores
• Micro and macromolecules
• 500,000 Dalton as collagen.
• 800.000 Dalton as hyaluronic acid
• Rearrange the structure in the cells of the
outer layer of skin
Electroporation
For electroporation we need:
• Membrane potential: 0.5-1.5 volts
• Disrupt lipid layer
• Open biological channels (aquous)
• These channels stay open several seconds
• Change polarity constantly
Background
The application of an electrical pulse momentarily disrupts
the cell membrane, allowing entry of product.
When the pulse ceases, the membrane returns to its original
structure, leaving retained product that has penetrated into
the interior of the cell in place.
Electroporation
• Electric doors called biological
channels
• Electric pulse changes
membrane polarity
• By slowly changing the
polarization we do not cause
electrolysis
• This disrupts lipid layer
Open Channels
Intercellular Communications
Cellular Penetration
Electroporation
• This effect lasts for seconds, therefore allowing the introduction of substances.
• Hydrofilic and hydrophobic molecules penetrate at the rate of 1g every 1 - 1.5
minutes.
• Continuous formation and appearance of biological channels
• Dramatically increases skin permeability.
Conclusion
• Electroporation is a process that uses high-voltage current to make cell membranes
permeable to allow the introduction of new materials.
• In a practical sense Electroporation is opening a door that allows us to insert
“products” into the body.
Methodology
• Research documents 4 times better results
when you do an exfoliation such as a peel
or micromicrodermabrasion before
Electroporation.
• This eliminates dead cells from the
Keratinic layer.
• This changes the skin potential and
makes it easier to create biological
channels.
Comparison with
500
400
300
200
100
0
No Microdermabrasion - No Dermoelectroporation
No Microdermabrasion - Dermoelectroporation Only
Microdermabrasion Only - No Dermoelectroporation
Microdermabrasion & Dermoelectroporation
Indications
Introduction of substances for:
• Skin treatment
• Cellulite
• Flaccidity
• Wrinkles
• Obesity
• Collagen production
Contraindications
• Pacemakers, heart valves or other active
implants.
• Epilepsy
• Pregnant women
• Immediately after Botox
• Skin outbreaks or on infected tissue
• Metal plates or other metal objects
• Ulcer or gastroenteritis
Wrinkles
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion.
Optional: Use a micro roller after microderm.
Select Protocol:
Power: 70% - 80%
Time of application: 20 – 25 minutes
Amount of product:
4 to 6 cc, divided into face, neck, and décolleté
Method:
Circular motion, soft and slow until completely
absorbed
Frequency of session:
One session a week during five to six weeks
After one session every two weeks for one month
A monthly session as maintenance
Décolleté
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion.
Optional: Use a micro roller after microderm.
Select Protocol:
Power: 70% - 80%
Time of application: 15 – 20 minutes
Amount of product: 4 cc
Method:
Circular motion, soft and slow until completely
absorbed
Frequency of session:
One session a week during five to six weeks
After one session every two weeks for one month
A monthly session as maintenance
Body Firming
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion.
Optional: Use a micro roller after microderm.
Select Protocol:
Power: 80% - 90%
Time of application: 15 – 20 minutes
Amount of product:
5 to 6 cc (depending if we have to reduce or reaffirm)
Method:
Circular motion, soft and slow until completely
absorbed
Frequency of session:
One session a week during six to eight weeks
After one session every two weeks for one month
A monthly session as maintenance
Breast Firming
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion or gentle enzyme peel.
Select Protocol:
Power: 80% - 90%
Time of application: 15 – 20 minutes
Amount of product: 3 to 4 cc
Method:
Circular motion, soft and slow until completely
absorbed
Frequency of session:
One session a week during six to eight weeks
After one session every two weeks for one month
A monthly session as maintenance
Facial Rejuvenation
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion.
Optional: Use a micro roller after microderm.
Select Protocol:
Power: 70% + Face, 30-40% Lips
Time of application: 20 – 25 minutes
Amount of product:
5 to 6 cc, divided into face, neck, and chest
(also hands)
Method:
Circular motion, soft and slow until completely absorbed
Frequency of session:
One session a week during three to four weeks
After one session every two weeks for one month
A monthly session as maintenance
Localized Obesity
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion.
Optional: Use a micro roller after microderm.
Select Protocol:
Power: 85% - 95%
Time of application: 15 – 20 minutes
Amount of product: 6 to 10cc
Method:
Circular motion, soft and slow until completely
absorbed
Frequency of session:
One session a week during six to eight weeks
After one session every two weeks for one month
A monthly session as maintenance
Cellulite
Instruction:
Clean and dry skin the skin.
Exfoliate by microdermabrasion.
Optional: Use a micro roller after microderm.
Select Protocol:
Power: 85% - 95%
Time of application: 15 – 20 minutes
Amount of product: 6 to 10cc
Method:
Circular motion, soft and slow until completely
absorbed
Frequency of session:
One session a week during six to eight weeks
After one session every two weeks for one month
A monthly session as maintenance
ELECTROPORATION
Onyx Medical, Inc.
1-800-333-5773
WWW.ONYXMEDICAL.COM