Download Tissues and Organs Comprising the Immune Response System

Tissues and Organs
Comprising the Immune
Response System
Where components of the immune response
originate,
differentiate,
meet,
interact,
and function
Folder Title: Tiss&Org(NoTP)
Updated: November 15, 2016
Interactions that have to take place in the
Immune Response:
Slides 3 to 9
Marcophage and T-Cell Kissing
APC and T-Cells
Macrophage to T-Cell Interaction
Helper T-cell B-Cell Interaction
CD4 co-receptor
B-Cell Activation
See figure 2-7,
Kuby, 4th Ed.,
p. 36
B-Cell: Small, Blast, and Plasma
See Figure 2-7,
Kuby 4th Ed.
p. 36
Where do these cells originate? Primary
Lymphoid Organs: Bone Marrow and Thymus
Where do they go to interact with each other
and with Targets?
Secondary Lymphoid Organs
Where do they carry out their functions?
Related to Figure
2-13, Kuby, 4th
Edition, p. 47
Primary and Secondary
Lymphoid
Animations
Produced for Kuby Immunology
(In Class Do animations from the Book CD)
Chapter 2 Animation. Cells and Organs.
Last Panel Shows Organs of Immune Response
Please put away all notes and any devices except
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Primary Lymphoid Organ:
The Thymus:
Where Lymphocytes are
Selected, Edited, and
“Educated”
Thymus Photo
Thymus Cartoon
Diagrammatic Cross Section of Thymus. Figure 2-14,
Kuby, 4th Edition, p. 48
ThymusCross
CD4 and CD8 in Thymus
To Here: Tuesday
November 12, 2015
Before Exam
Secondary Lymphoid Organs:
Where Cells and Targets Meet.
Where Lymphocytes & Myelocytes
Differentiate and Mature
MALT and M-Cells
See Figure 2-22,
Kuby 4th Ed.
p. 56
MALT and IgA
See Figure 2-22
Kuby, 4th Ed.
p. 56
Animations
Produced for Kuby Immunology
Chapter 11 Animation. Signal Transduction
Signal, Receptors, and co-receptor Pairs
Secrets of Signaling
1. How Do You Knock on a Door? Why that way?
2. Why can the police officer directing traffic blow his whistle only
once and get a response?
3. Why does your computer say “Are You Sure You Want to Delete
File XYZ?”
4. When the class was directed to do “Bunny Ears” behind the person
next to them, only a few persons responded. Why was that?
5. A different set of persons responded when asked to raise their right
hand. Why was that?
6. No one responded when the class was asked to stand up. Why?
Secrets of Signaling (2)
7. Only the people in the front of the room responded when I invited
the class to do what all classes would love to do to a pain-in-the neck
Prof. Why was that?
8. I asked the class in plain English to raise both hands but no one
complied. Why is that? What did that illustrate about properties of
signaling.
9. Why does the T-Helper Cell physically attach itself to the
macrophage? What is the advantage of that?
10. Why does the T-Cell use a co-receptor in addition to its T-Cell
Receptor?
11. Why does a T-Cell look for a second signal (B7 to CD28) before
the T-cell will act?
Costimulatory signals: B7 –
CD28
Costimulatory Signals: T-Cell to
MPh
Cancer Eluding Costimulatory
Signal
Cell Surface Markers
Functional Surface Molecules that Allow
Specific Cells to perform specific tasks.
Become identifiers of Specific Cells
Analogy of a Basketball Player
Person 1:
Person 2:
Person 3:
Person 4:
Person 5:
5 ft 5”, 160 lbs bespectacled old geezer
5 ft 11”, 280 lbs, 22-year old guy, all muscle
5 ft 5”, 135 lbs, 20-year-old woman
7 ft 1” , 200 lbs, 22 year-old guy
5 ft 8”, 150 lbs, 22 year-old guy
Which Person:
A. Is a varsity basketball player?
B. Plays on the women’s field-hockey team?
C. Is a boring old professor?
D. Is a varsity football player for SU?
E. Is a men’s cross-country runner?
1C; 2D; 3B; 4A; 5E
CD’s on B, T, and NK Cells
Surface
Determinants on
B-Cells:
Immunoglobulins
B7
Surface Determinants
on T-Lymphocytes:
Note: CD4 TH
CD8 TC
CD28 and CD3 Both.
NK Determinants:
CD56
CD16
CD8
CD Markers in Leukemia
Diagnosis
Staging
Prognosis
Treatment
(“ALL” = Acute Lymphocytic
Leukemia)
T-Cell ALL Markers
Kuby, 5th Edition
p. 157
Pre-B-Cell ALL Markers
(“ALL” = Acute Lymphocytic
Leukemia)
Kuby, 5th Edition
p. 157
(“CLL” = Chronic
Lymphocytic Leukemia)
B-Cell CLL Markers
Kuby, 5th Edition
p. 157
Leukocyte Recirculation
How Do Circulating Hematopoietic
Cells “Know” Where They Are?
How Do they Stick?
How Do they Move?
See Animation on CD
Chapter 13 Leucocytes
Primary Lymphoid Organs
Lymphocyte Recirculation
Secondary Lymphoid
Organs
Leucocytes in Capillaries
Cartoon
Leucocytes in Capillaries Photo
Extravasation (Getting out of the Blood Stream)
of Naïve T-Cell into Lymph Node
CAM =
Cell Adhesion Molecule
Adhesion: Leucocytes to
Endothelium
Cell Surface Receptors and Signals in Leucocyte Extravasation
(Non-inflamed Endothelium)
CAM = Cell Adhesion Molecules
Membrane of Circulating
Leucocytes
“CHO” = carbohydrates
Lectins = carbohydrate
binding proteins
Endothelial Cells Lining
Capillaries
CAMinCap Adhesion in
Capilaries
(Inflamed Endothelium)
Neutrophils trails guide virus-specific CD8+ T cell migration.
Chemokine from
Uropod of
Neutrophils
Kihong Lim et al. Science 2015;349:aaa4352
Published by AAAS
Neutrophils trails guide virus-specific CD8+ T cell migration.
(CXCL12 is a chemokine: chemical agent attracting cell movement)
Virus-specific Cytotoxic T-Cell
Blebs containing CXCL12 from Uropod
(Trailing membrane region) of Neutrophil
“Bread-crumb” trail
Kihong Lim et al. Science 2015;349:aaa4352
Cytotoxic T-Cell migration through trachea to virus-infected cells
Published by AAAS
Please put away all notes and any devices except
for your Turning Point NXT Transmitter.
No papers or computers on your desk please.
No communication between or among students.
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Slides Beyond this Point Not included in
BIO447, Fall, 2016
Animations
Produced for Kuby Immunology
http://bcs.whfreeman.com/immunology6e/
Or search “Kuby Immunology”,
Click on “Kuby Immunology 6e” ,
go to Student Resources
Chapter 2: Cells
Cell Death (Also chapter 10)
Chapter 11: Signal Transduction
Chapter 13: Leucocyte Extravasation
Actin Cytoskeleton Linkage to Collagen
Extra-cellular Matrix
Figure 19-14 ECB
1998, p. 603
Collagen
Fibronectin
Integrin
Plasma Membrane
Actin Microfilament
MFtoECM
Tissue-target Cells in Red
Leucocyte CAM in Blue
CAMFamly
On Endothelial
Cells
On Neutrophils
On Mucosal Epithelium
On Leucocytes
On Endothelial
Cells
On Leucocytes
CamFamly
On Endothelial
Cells
Families of
Cell Adhesion
Molecules
Kuby, 3rd Ed.
Figure 15-2b
Animations
Produced for Kuby Immunology
OnLine Link
http://bcs.whfreeman.com/immunology6e/
In Class Use Book CD:
Chapter 2: Cells of the Immune-system (Static)
Cell Death : (Animated)
Chapter 11: Signal Transduction (Animated)
Chapter 4 : Immunoglobulins (Static)
Chapter 13 Animation. Leukocyte Extravasation (Animated)
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CD Antigen Table
CDMarks
CD Antigens Color Coded