Download PATHOGEN SAFETY DATA SHEET Streptococcus agalactiae

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PATHOGEN SAFETY DATA SHEET
Streptococcus agalactiae
CHARACTERISTICS
Morphology
Growth Conditions
Gram-positive facultative anaerobe that is usually
found in pairs or chains.
Can be placed in various growth media and incubated
at 37oC.
HEALTH HAZARDS
Host Range
Modes of
Transmission
Signs and
Symptoms
Infectious Dose
Incubation Period:
Humans and Animals.
Hand-to-mouth and aerosol transmission is common.
10-30% of women are positive in their genital tracts
although the bacterium is normally a resident of the
gastrointestinal tract.
Shaking chills, chest pain, dyspnea, fever, productive
cough, and sinusitis. Can cause middle ear infections.
Can progress to pneumonia, bacteremia, meningitis.
Unknown
For early onset disease, less than 7 days. For late onset
disease, the incubation period is unknown.
MEDICAL PRECAUTIONS/TREATMENT
Prophylaxis
Vaccines
Penicillin or ampicillin have been shown to reduce
transmission and morbidity to the neonate
None.
Treatment
Surveillance
MSU Requirements
Antibiotic treatment.
Monitor for symptoms of infection.
Report any exposures
LABORATORY HAZARDS
Laboratory
Acquired Infections
(LAIs)
Sources
78 documented cases since 1999
Sputum, blood, respiratory secretions, throat swabs,
parenteral inoculation.
SUPPLEMENTAL REFERENCES
Canadian MSDS:
BMBL:5th Edition
CDC
http://www.phac-aspc.gc.ca/lab-bio/res/psdsftss/index-eng.php
http://www.cdc.gov/biosafety/publications/bmbl5/BM
BL.pdf
http://www.cdc.gov/groupbstrep/clinicians/clinicaloverview.html
CONTAINMENT REQUIREMENTS
BSL2
For all procedures involving known or potentially
infected cultures.
ABSL2
For all procedures utilizing infected animals
SPILL PROCEDURES
Small
Notify others working in the lab. Remove and don
new PPE. Cover area of the spill with absorbent
material and add 10 % Bleach. Allow 30 minutes
hour of contact time. After 30 minutes and then
cleanup and dispose of materials.
Large
For assistance, contact MSU's Biosafety Officer (406994-6998) or Safety and Risk Management (406-9942711).
EXPOSURE PROCEDURES
Mucous membrane
Other Exposures
Reporting
Medical Follow-up
VIABILITY
Disinfection
Inactivation
Survival Outside Host
Flush eyes, mouth or nose for 5 minutes at eyewash
station.
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete
a first report of injury report, and submit to Safety
and Risk Management.
During business hours:
Montana Occupational Health
2075 Charlotte St. Suite 3
Bozeman, MT
After business hours:
Bozeman Deaconess Hospital
Emergency Room
915 Highland Blvd
Bozeman, MT
Susceptible to 10 % Bleach, 70 % ethanol, and 2 %
gluteraldehyde.
Inactivated by moist heat (15 minutes at 121oC) and
dry heat (1 hour at 160-170oC).
Has been found to survive months in dry dust in
buildings. It can survive in milk at -20oC for 4 weeks
and in fish tissues at 700C for at least 9 months.
PERSONAL PROTECTIVE EQUIPMENT (PPE)
Minimum PPE
Requirements
Additional
Precautions
At minimum, gloves, closed toed shoes, lab coat,
and appropriate face and eye protection prior to
working with S. agalactiae. Additional PPE may be
required depending on lab specific SOPs.
All procedures that may produce aerosols, or
involve high concentrations or large volumes should
be conducted in a biological safety cabinet (BSC).