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DO NOW • How can muscle cells maintain (or keep) a high concentration of potassium inside the cell? • If a cell increases or decreases in volume, what type of transport must happen? • Building blocks of carbohydrates? • Building blocks of proteins? • Function of the nucleus? Diffusion Through the Membrane State Lab Selectively Permeable • The cell membrane is selectively permeable because it only lets certain molecules pass through the membrane • Glucose, water, O2, CO2, amino acids, and other small molecules are permeable to the membrane • Starch, proteins, and other LARGE molecules are NOT permeable to the membrane. – They must be DIGESTED, or broken down. • The state lab was all about DIFFUSION!!!!!! • We used an “artificial cell” to observe the movement of glucose, starch, and starch indicator. • An indicator is used to test for the presence of a chemical substance. • Starch indicator (iodine) is amber brown. It will turn blue/black in the presence of starch. • The beaker was LEFT ALONE for 20 minutes AMBER WHITE • An indicator is used to test for the presence of a chemical substance. • Starch indicator (iodine) is amber brown. It will turn blue/black in the presence of starch. • The beaker was LEFT ALONE for 20 minutes • After 20 minutes, the bag turned black, the beaker stayed amber AMBER AMBER WHITE BLACK • The bag turning black told us that starch indicator (iodine) moved into the cell. • The water staying amber told us that starch DID NOT move out of the cell. But what about glucose?.... But what about glucose?.... • In order to determine if glucose moved, a glucose indicator test had to be performed. • Glucose indicator is clear blue, and IT MUST BE HEATED. • Glucose indicator will turn red, yellow, or green (traffic light colors) if glucose is present. • A sample of the amber water was taken from the beaker and heated with glucose indicator. • The test was POSITIVE. Glucose WAS present in the beaker. I I G I I S G S GS G S I I I I G I I I I S G S GS G S I I S G S IG G S S S I G I G I G I Conclusion of the Lab • Glucose and starch indicator were PERMEABLE to the dialysis tube membrane. • Starch was NOT permeable to the membrane.
