Download GMS 6062 Pro Traf 02

GMS 6062 Protein Trafficking I
2002
Tuesday and Thursday, 1:30-3:30
Director: J. Aris
Schedule
Date
Feb. 12
Feb. 14
Topic
mRNA Localization
Secretory Protein Degradation
Feb. 19
Feb. 21
Golgi Quality Control
Golgi Transport
Feb. 26
Feb. 28
Golgi and Apoptosis
Polarized Cells
Mar. 12
Mar. 14
Vesicle Motors
Endocytosis
Mar. 19
Mar. 21
Lipids - PM Transport
Lipids - Protein Targeting
Readings
Feb. 12
mRNA Localization
Review:
Jansen, R. P. 2001. mRNA localization: message on the move, Nat Rev Mol Cell Biol 2, 247-56.
Paper:
Gu, W., Pan, F., Zhang, H., Bassell, G. J., and Singer, R. H. 2002. A predominantly nuclear protein
affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons, J Cell Biol 156, 4151.
Additional Reference(s):
Palacios, I. M. 2002. RNA processing: splicing and the cytoplasmic localisation of mRNA, Curr Biol
12, R50-2.
Feb. 14
Secretory Protein Degradation
Review:
Plemper, R. K., and Wolf, D. H. 1999. Retrograde protein translocation: ERADication of secretory
proteins in health and disease, Trends Biochem Sci 24, 266-70.
Paper:
Jarosch, E., Taxis, C., Volkwein, C., Bordallo, J., Finley, D., Wolf, D. H., and Sommer, T. 2002.
Protein dislocation from the ER requires polyubiquitination and the AAA-ATPase Cdc48, Nat Cell Biol
28, 28.
Additional Reference(s):
Rabinovich, E., Kerem, A., Frohlich, K. U., Diamant, N., and Bar-Nun, S. 2002. AAA-ATPase
p97/Cdc48p, a cytosolic chaperone required for endoplasmic reticulum-associated protein
degradation, Mol Cell Biol 22, 626-34.
Ellgaard, L., and Helenius, A. 2001. ER quality control: towards an understanding at the molecular
level, Curr Opin Cell Biol 13, 431-7.
Feb. 19
Golgi Quality Control
Review: (None)
Paper:
Reggiori, F., and Pelham, H. R. 2002. A transmembrane ubiquitin ligase required to sort membrane
proteins into multivesicular bodies, Nat Cell Biol 14, 14.
Additional Reference(s):
Munro, S. 1998. Localization of proteins to the Golgi apparatus. Trends Cell Biol 8:11-5.
Feb. 21
Golgi Transport
Review:
Pelham, H. R. 2001. Traffic through the Golgi apparatus, J Cell Biol 155, 1099-101.
Paper:
Martinez-Menarguez, J. A., Prekeris, R., Oorschot, V. M., Scheller, R., Slot, J. W., Geuze, H. J., and
Klumperman, J. 2001. Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent
with the cisternal progression model of intra-Golgi transport, J Cell Biol 155, 1213-24.
Mironov, A. A., Beznoussenko, G. V., Nicoziani, P., Martella, O., Trucco, A., Kweon, H. S., Di
Giandomenico, D., Polishchuk, R. S., Fusella, A., Lupetti, P., et al. 2001. Small cargo proteins and
large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of
cisternae, J Cell Biol 155, 1225-38.
Additional Reference(s):
Pelham, H. R. 1998. Getting through the Golgi complex. Trends Cell Biol 8:45-91.
Mironov, A. A., Weidman, P., and Luini, A. 1997. Variations on the intracellular transport theme:
maturing cisternae and trafficking tubules, J Cell Biol 138, 481-4.
Feb. 26
Golgi and Apoptosis
Review (General):
Kaufmann, S. H., and Hengartner, M. O. 2001. Programmed cell death: alive and well in the new
millennium, Trends Cell Biol 11, 526-34.
Paper:
Lane, J. D., Lucocq, J., Pryde, J., Barr, F. A., Woodman, P. G., Allan, V. J., and Lowe, M. 2002.
Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation
during apoptosis, J Cell Biol 156, 495-509.
Feb. 28
Polarized Cells
Review:
Nelson, W. J., and Yeaman, C. 2001. Protein trafficking in the exocytic pathway of polarized epithelial
cells, Trends Cell Biol 11, 483-6.
Paper:
Moskalenko, S., Henry, D. O., Rosse, C., Mirey, G., Camonis, J. H., and White, M. A. 2002. The
exocyst is a Ral effector complex, Nat Cell Biol 4, 66-72.
Additional Reference(s):
Short, B., and Barr, F. A. 2002. Membrane Traffic: Exocyst III - Makes a Family, Curr Biol 12, R18-20.
Mar. 12
Vesicle Motors
Review:
Kamal, A., and Goldstein, L. S. 2002. Principles of cargo attachment to cytoplasmic motor proteins,
Curr Opin Cell Biol 14, 63-8.
Paper:
Tai, A. W., Chuang, J. Z., and Sung, C. H. 2001. Cytoplasmic dynein regulation by subunit
heterogeneity and its role in apical transport, J Cell Biol 153, 1499-509.
Additional Reference(s):
Goldstein, L. S. 2001. Molecular motors: from one motor many tails to one motor many tales, Trends
Cell Biol 11, 477-82.
Hammer, J. A., and Wu, X. S. 2002. Rabs grab motors: defining the connections between Rab
GTPases and motor proteins, Curr Opin Cell Biol 14, 69-75.
Shah, J. V., and Cleveland, D. W. 2002. Slow axonal transport: fast motors in the slow lane, Curr Opin
Cell Biol 14, 58-62.
Mar. 14
Endocytosis
Review:
Pfeffer, S. R. 2001. Rab GTPases: specifying and deciphering organelle identity and function, Trends
Cell Biol 11, 487-91.
Paper:
P. Barbero, L. Bittova, and S. R. Pfeffer. 2002. Visualization of Rab9-mediated vesicle transport from
endosomes to the trans-Golgi in living cells. J. Cell Biol. 156:511–518
Additional Reference(s):
M. Deneka and P. van der Sluijs. 2002. ‘Rab’ing up endosomal membrane transport. Nature Cell
Biol. 4:33-35 (News and Views)
S. De Renzis, B. Sönnichsen and M. Zerial. 2002. Divalent Rab effectors regulate the subcompartmental organization and sorting of early endosomes. Nature Cell Biol. 4:124-33
Mar. 19
Lipids - PM Transport
Review:
Bankaitis, V. A. (2002). Cell biology. Slick recruitment to the Golgi, Science 295, 290-1.
Paper:
Baron, C. L., and Malhotra, V. 2002. Role of diacylglycerol in PKD recruitment to the TGN and protein
transport to the plasma membrane, Science 295, 325-8.
Additional Reference(s):
Edidin, M. 2001. Shrinking patches and slippery rafts: scales of domains in the plasma membrane,
Trends Cell Biol 11, 492-6.
Mar. 21
Lipids - Protein Targeting
Review:
Sato, T. K., Overduin, M., and Emr, S. D. 2001. Location, location, location: membrane targeting
directed by PX domains, Science 294, 1881-5.
Paper:
Cheever, M. L., Sato, T. K., de Beer, T., Kutateladze, T. G., Emr, S. D., and Overduin, M. 2001. Phox
domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes, Nat Cell Biol 3,
613-8.
Additional Reference(s):
Simonsen, A., Wurmser, A. E., Emr, S. D., and Stenmark, H. 2001. The role of phosphoinositides in
membrane transport, Curr Opin Cell Biol 13, 485-92.