Download Steps to follow to obtain data required for using the

Steps to follow to obtain data required for using the Biomart_to_SyntenyTracker_format tool
1. Click on the “Martview” option in the biomart website (http://www.biomart.org/index.html). In the
“choose database pull down” menu, please select “ENSEMBL 53 GENES (SANGER UK)” option. Another
pull down menu, “choose database” appears. Please select a genes set of interest (reference genome) as
shown below.
2. On the left side pane, click on the “Attributes” link. Of the options displayed on the central pane,
choose “Homologs”. Expand the “Gene” window and “Orthologs” window of any other species of
interest (target genome), select chromosome name, start and end coordinates in both “Gene” and
“Orthologs” windows respectively and select “Orthology type” in “Orthologs” window. In addition to the
default IDs that were selected in the “Gene” window please select another ID either in “Gene” or
“Orthologs” windows.
3. On the left side pane, click on the “Filters” link. Expand the “Multi species comparisons” window on
the central pane. Select “Homolog filters” and choose the orthologous genes set of the species that was
earlier selected in the “Attributes” link. In addition select the option “only”.
4. Click on the “Results” button. Export all the unique results to a TSV file.