Download Biacore™ systems for more information and faster decisions.

Biacore™ systems
for more information
and faster decisions.
Label-free interaction analysis.
More answers:
Deeper insights into
biological realities
Biological processes are “real-time” events, driven and regulated by dynamic interactions
between key molecules.
End-point techniques such as ELISA offer a snapshot view of interactions providing only basic
information such as overall binding strength (affinity). The affinity depends on the ratio of
on- and off-rates so that equal affinity interactions can have very different kinetic properties,
resulting in different biological responses.
Biacore systems can provide key data in real-time to discriminate these crucial differences,
even for interactions where challenging targets are involved.
Biacore systems are designed to help you to generate decisive, information-rich data, that will
help answer key questions concerning the nature of the binding:
1.How strong?
Affinity is a steady-state measurement made at equilibrium of a binding event and reflects
the strength of an attraction between molecules.
2.How fast?
Binding kinetics determine how fast/slow a complex forms or dissociates within a given
time span and allow calculation of association and dissociation rate constants.
3.How much?
Qualitative and quantitative determination of active analyte binding to a target protein.
4. How specific?
Is the molecule specific for its target? Does the antibody recognize multiple derivatives?
The flexibility in the Biacore assay design allows rapid assessment of cross-reactivity
and specificity.
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More insights: Real-time
monitoring of binding events
delivers deeper understanding.
Biacore systems provide valuable information from high-quality molecular interaction data in a range of
fields: from biological research, biotherapeutics and small-molecule drug discovery and development,
through immunogenicity studies and vaccine development to quality control. The sensitivity and
performance of Biacore systems enables detailed characterization of molecular interactions even for
challenging targets such as membrane proteins. Biacore systems allow you to:
Association: Interactants in solution bind to molecules on sensor chip surface.
• Understand the relationship between molecular interaction and function.
• Screen for hits and optimize leads based on selectivity, affinity, and kinetics.
• Examine interactions of ions, small molecules, and multidomain proteins or viruses with targets.
• Screen and characterize antibodies and proteins based on yes/no binding, affinity, and kinetics from
the fastest on-rates to the slowest off-rates.
• Quantitate protein by measuring the concentration of active protein with retained biological function.
Biacore systems
Molecules
Methods
Fields
Antibodies
Affinity
Basic research
Proteins
Kinetics
Peptides
Screening
Biotherapeutics discovery
and development
Compounds and fragments
Specificity
Small-molecule discovery
Nucleic acids
Concentration
Ions
Epitope binning
Biotherapeutics manufacturing
and quality control
Virus
Immunogenicity
Thermodynamics
Vaccine design and
development
Dissociation: Binders allowed to dissociate from molecule on sensor chip surface.
Association
Dissociation
The binding (association) and dissociation of molecules to the sensor chip surface are monitored in real-time and displayed in a sensorgram.
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More information: Biacore
systems provide high-quality
data in key applications
Understanding the nature of interactions between molecules is fundamentally important for increased
understanding of biological processes. Biacore surface plasmon resonance (SPR)-based sensors are
widely used to study molecular interactions in real-time and are applied as powerful tools in research
and drug discovery throughout to quality control and drug-release testing.
Research
Characterization of
molecular interactions
Structural/functional studies
Biotherapeutics and small molecule discovery
Biotherapeutics development and manufacturing
Screening
Critical quality
attributes (CQA)
Characterization
Boost your protein research and enable the design of new
and innovative studies.
Screen and optimize your compounds
based on direct label-free binding data.
• Real-time monitoring of binding events gives a deeper
understanding of the dynamics of molecular interactions.
• Screen and select hits based on
selectivity, affinity, and kinetics.
• Kinetics, affinity, transition state thermodynamics, and yes/
no binding data help you prove your hypotheses and make
dependable conclusions.
• Optimize lead compounds through
increased understanding of
molecular mode of action.
Lead optimization
Design your vaccines and
biotherapeutics with desired
binding properties.
• Accurate ranking and selection
of antibodies and proteins based
on yes/no binding, affinity, and
kinetics—from very fast on-rates
to very slow off-rates.
Potency
Reliably define CQA and monitor drug release parameters.
• Fingerprint analysis of drug interaction and stability, even
for new drug formats such as bispecific antibodies.
• Novel opportunity for comparability and biosimilar studies.
• Seamless integration into GxP-regulated workflows.
Application and technical support – Training – Instrument service – Validation services
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Release testing
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Get more from your system.
Biacore service and support.
GE Healthcare Life Sciences is committed to supporting customers with innovative tools,
analytics, and everyday lab reagents to uncover new insights in research or to discover, develop
and manufacture drugs.
More than 30 000 scientific publications and still counting...
Thousands of Biacore systems are installed globally and more than 30 000 scientific articles
have been published in peer-reviewed journals where Biacore systems have been featured for a
wide range of applications.
Experienced application specialists and service experts
Our instrument service and application support is performed by specially trained service experts
available close to you. They can help you to get the most out of your Biacore system for all
applications and improve efficiency by minimizing your equipment system downtime. This allows
you to focus on your work delivering reliable binding analysis results. To learn more about assay
setup and data interpretation, GE offers a wide range of self-training tools, classroom trainings,
handbooks, and lab protocols.
Biacore club: Connect. Learn. Share.
Biacore club is an online platform for Biacore system users that provides:
• Easy access to information about your Biacore system.
• Ability to explore a rich content library including videos and handbooks.
• Exclusive offers on Biacore products.
• Access to presentations and materials from our regional user days and DiPIA conferences.
Join Biacore club to connect, share, and get access to exclusive content and offerings.
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Consumables and software
that deliver more
GE Healthcare Life Sciences has developed a range of tools designed specifically to make Biacore
assays as easy and reliable as possible, suitable for users of all levels of experience. The complete
toolbox is backed by stringent development, production methods and quality control. Selected
Biacore systems facilitates compliance with worldwide regulatory expectations by offering
Validation Services and 21 CFR Part 11 functionality.
Get started quickly at any experience level
Use predefined Biacore run methods with application-relevant settings to rapidly set up your assay,
or utilize the full flexibility of the control software to tailor methods to your specific needs.
Reduce time to conclusive results
The efficient evaluation software is designed to provide data overview, speed up data evaluation,
and to shorten time to results. Quality control tools help you to assess high-quality kinetic and
affinity data for improved interpretation of results.
A sensor surface for every need
Biacore sensor chips offer support for analysis of a wide range of interactions, backed up with a
selection guide to help you select and use the right tools for the application.
Save time and effort using kits and ready-made consumables
Our capture kits significantly reduce assay development time and gives consistent capture of
antibodies as well as the most common tags. Ready-made buffers and solutions are developed for
your convenience and to ensure reliable results.
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More choices for label-free
interaction analysis.
From research through drug discovery and development to QC.
Choose the right system for your needs.
Biacore 8K
Biacore X100
High sensitivity and efficiency
Delivering high-quality binding data to meet the
toughest challenges in small-molecule/biotherapeutic
screening and characterization.
Reliable, built-in knowledge base
Reliable insights into biological processes in multiuser
environments/small-scale interaction analysis.
Biacore S200
Biacore 4000
Outstanding sensitivity for cutting-edge applications
Our most sensitive SPR-based sensor enables confident
analysis of the smallest fragments, even against
challenging targets such as GPCRs in LMW/fragment
drug discovery.
Convenient and productive
Large-scale, label-free analysis in drug discovery
providing high throughput without compromise
on data quality.
Biacore T200
Biacore C
Reliable, versatile, and precise
Versatile system for high-quality characterization of
molecular interactions—from ions to viruses.
Quality, compliant, and secure
The world’s first SPR instrument dedicated to rapid
determination of concentration in GxP environments.
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GE Healthcare Bio-Sciences Corp.
100 Results Way
Marlborough, MA 01752
USA
For local office contact information,
visit gelifesciences com/contact.
gelifesciences.com/biacore
GE, the GE Monogram, and Biacore are trademarks of General Electric Company.
© 2016 General Electric Company. All goods and services are sold subject to
the terms and conditions of sale of the company within GE Healthcare which
supplies them. A copy of these terms and conditions is available on request.
Contact your local representative for the most current information.
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