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173 Looking at Types of Cells While we will focus in depth on human body cells, it’s useful and interesting to know that all living things on Earth are divided into a few basic groups based on the kinds of cells they have. We will use the microscope to look at representatives of the Animal Kingdom, Plant Kingdom and Protist Kingdom. The Animal Kingdom, represented by....human cheek cells! You are a member of the Animal Kingdom, and your cheek cells are easy to examine. 1. Put on gloves. 2. Use a toothpick to gently scrape the inside of your cheek. (Why are we doing this?) 3. Wipe the contents on a small portion of a microscope slide. 4. Ask me for a drop of methylene blue stain. (Why are we adding a “stain”?) 5. Add a cover slip to the slide. 6. Examine the slide under the microscope (low power, then medium power, then high power) for evidence of your cells. (What do you expect your cheek cells to look like?) 7. Draw your cheek cells here. See if you can label the cell membrane, nucleus, and cytoplasm. You may also see some other organelles as well. Why do you think that animal cells have a flexible cell membrane as their outer covering instead of a sturdy cell wall like you will soon see in plant cells? 8. OPTIONAL FUN: What is the effect of salt on your cheek cells? Introduce a drop of salt water onto your slide just as you did with the stain. Wait a few minutes. What happens to the cells? Why is this? 174 The Plant Kingdom, represented by... Anacharis (seaweed), Onion and Lettuce! Here are pictures of cells of three plants – (Anacharis) seaweed, onion and lettuce: http://science.exeter.edu/jekstrom/WEB/CELLS/Epith/JPEG/ http://www.ecok.edu:9045/repository/unmanaged_content/biology/russo/ http://www.luc.edu/depts/biology/111/ 175 1. Make slides of each of the three plant types and observe them under the microscope. 2. Determine which photograph depicts which plant type and label each photograph correctly. 3. In the Anacharis (seaweed) photograph, label cell wall, cell membrane (tricky!!), cytoplasm and chloroplasts. In the slide you prepare, you may also see the nucleus of some cells and vacuoles in other cells. Draw those in on the photograph of the Anacharis cells. 4. In the onion photograph, label cell wall, nucleus, cytoplasm and cell membrane (tricky!!). 5. In the lettuce photograph, label cell wall, cell membrane (tricky!!), nucleus (very tricky!!), cytoplasm, and chloroplasts. Thinking questions: 1. Why do plant cells have a sturdy cell wall in addition to their cell membrane? 2. Why do Anacharis cells and the cells of many other plants have the green sacs called chloroplasts in them? 3. Why, then, are there no chloroplasts in the section of onion you looked at? 4. Look closely at the lettuce. Notice that there are a few special round cells in addition to the squarish or blobby cells. Which cells in the lettuce contain chloroplasts – the round or the square? What do you think these round cells might be for? 5. OPTIONAL FUN: What is the effect of salt on plant cells? Add a drop of salt water to the plant cells. Wait a few minutes. What happens to the cells? 176 Microscopic Life in the Park Pond A drop of pond water can yield hundreds of exciting and interesting organisms. These include microscopic animals, microscopic plants and a group of single-celled creatures called Protists. A few common types of protists you might find are Paramecium, Vorticella and Amoeba. Many of “The Far Side” cartoons feature Protists! Obtain a drop of pond water that contains some chunks of debris and place this on a slide with a cover slip. Here are some things you might see: Organism Comments These are members of the Animal Kingdom because Interesting adaptations include Bristle Worm http://www.environmentalleverage.com/Bristle%20Worms.htm http://mblaquaculture.com/assets/images/content/photo_Daphnia _magna.jpg These are members of the Animal Kingdom because Interesting adaptations include Daphnia (water flea) 177 http://cache.eb.com/eb/image?id=9298&rendTypeId=4 These are members of the Plant Kingdom because Interesting adaptations include Various species of Algae These are members of the Protist Kingdom because http://serc.carleton.edu/images/microbelife/k12/cedarswamp/A WCS_microbes.jpg Various species of Protists http://serc.carleton.edu/images/microbelife/k12/cedarswamp/A WCS_microbes.jpg Interesting adaptations of Protists include More Protists What Kingdom do you think this belongs to? Interesting adaptations include Your discovery here What Kingdom do you think this belongs to? Interesting adaptations include Your discovery here 178 Questions: 1. Now that you’ve seen some cells firsthand, what do you think is a good definition of “cells”? 2. What are some differences that you saw between plant and animal cells? Why do these differences exist? 3. Why are our bodies made up of so many cells? Why are there not, say, a hundred or a few thousand? 4. One of the big remaining mysteries of human biology is the issue of cell differentiation. When a sperm cell fertilizes an egg and a little embryo is first developing, it is just a ball of identical cells. Somehow, as these cells divide, these identical early cells (often called stem cells) differentiate and turn into different cells like cheek cells, blood cells, heart cells, pancreas cells, etc. How do you think stem cells “know” what kind of cell to turn into? (I know this is a hard question! I’m just curious about your ideas.)