Download Bioinstrumentation - E

NEHRU ARTS AND SCIENCE COLLEGE
DEPARTMENT OF MICROBIOLOGY
QUESTION BANK
Class: I B.Sc., Microbiology
Batch: 2016-2019
Subject: Bioinstrumentation
UNIT – I
1. Buffers are mixtures of
a) Strong acid and strong base
b) Strong acid and weak base
c) Weak acid and their conjugate base
d) Weak base and their conjugate acid
2. As the pKa of an acid increases, the acid will be
a) More weaker
b) More stronger
c) Converted to neutral solution
d) Converted to basic solution
3. If a solution has to be a buffer, its pH should be
a) At its pKa value
b) At its Ka value
c) At 7
d) At 14
4. Normal pH of blood is
a) 7.0
b) 7.2
c) 7.3
d) 7.4
5. What is the molecular weight of water?
a) 10 g/ mol
b) 15 g/ mol
c) 18 g/ mol
d) 20 g/ mol
6. pH of buffer solution depends upon concentration of
a) acid (H+)
b) conjugate base (OH-)
c) salt
d) both A & B
7. The calomel electrode contains the saturated ________ solution
a) KCL
b) HCL
c) NaCl
d) None of the above
8. When the H+ ion concentration increases, pH values ________
a) Decrease
b) Increase
c) Equals
d) Stabilizes
9. Solution are the examples of
a) Compounds
b) Homogenous mixture
c) Heterogenous mixture
d) Colloids
10. The amount of solute present in a given amount of solvent or solution is called
a) Concentration
b) Molarity
c) Molality
d) Normality
11. Isotonic solution must have the same
a) Density
b) Normality
c) Volume
d) Osmotic pressure
12. A solution of known strength or concentration is called
a) Standard solution
b) Normal solution
c) Molal solution
d) Suspension
13. The number of moles of solute dissolved per kilogram of solvent is called
a) Mole fraction
b) Normality
c) Molality
d) Concentration
14. A solution is made by dissolving some salt in a beaker of water. The salt is referred to as the
a) Solute
b) Filtrate
c) Solution
d) Solvent
15. When KCl dissolves in water, the following will be produced
a) K and Cl
b) K+ and Clc) K and Cl2
d) K+ and Cl2
5X5=25
1. Write short notes on buffers
2. Write about acid buffers
3. Write a note on basic buffers
4. What are normal solution , explain
5. Give a brief note on molar solution
3X8 =24
1. Define buffers. Explain about acid and basic buffers
2. Define pH and write about pH meter and its applications with a labeled diagram
3. Explain about normal and molar solutions in detail
UNIT II
1. Which of the following is a biological indicator in autoclaving?
2.
3.
4.
5.
6.
7.
8.
(1) Bacillus stearothermophilus (2) Clostridium (3) Special tape (4) Both a & b
Tyndallisation is otherwise known as _________.
(1) Fractional steam sterilization (2) Flash pasteurization (3) Ultra high
tempaerature (4) Dry heat sterilization
Temperature and time period for dry heat sterilization is ________.
(1) 160°C to 170°C & 2 to 3 hrs (2) 170°C to 180°C & 2 to 3 hrs (3) 150°C to 160°C &
2 to 3 hrs (4) 180°C to 190°C & 2 to 3 hrs
Depth filters are made up of _______.
(1) Diatomaceous earth (2) Porcelain (3) Asbestos(4) All
Laminar flow cabinets employ ________.
(1) Depth filter (2) Membrane filter (3) HEPA (4)Chamberlain
___________ is dry air type sterilizer
(1) Autoclave (2) Hot air oven (3) Membrane filter (4) None
Hot air oven is operated at a temperature of ______
(1) 160°C (2) 180°C (3) 160°C - 180°C (4) 150°C-170°C
Laboratory glasswares are sterilized by
(1)Autoclave (2) Hot air oven (3) Membrane filter (4) none
9. Steam under pressure is the working principle of ________
(1) Autoclave (2) Hot air oven (3) Incubator (4)Incinerator
10. Autoclave is usually operated at a pressure of ______
(1) 10 lb/Sq.inch (2) 15 lb/Sq.inch (3) 20 lb/Sq.inch(4) 25 lb/Sq.inch
11. Autoclave kills the ________ of microbes
(1) Vegetative cells (2) Spores (3) Both a & b (4)Filaments
12. Media and canned foods are sterilized by _______
(1) Autoclave (2) Hot air oven (3) Incubator (4)Incinerator
13. Method of incubation of culture depends upon __________
(1) Temperature (2) oxygen (3) Both a & b (4) Either a or b
14. Temperature of an incubator is maintained constant by _______
(1) Cooling coil (2) Water (3) Thermostat (4) Microbe
15. Synchronous uniform suspension of cells can be obtained by _________
(1) Autoclave (2) Incubator (3) Metabolic shaker (4)All
16. The indication of biological pollution in water is measured as ______
(1) BOD (2) COD (3) TDS (4) All
17. BOD is expressed as ______
(1) cubic sq (2) ppm (3) dl (4) lb/sq.inch
18. _______ is used to destroy contaminated clothings, pathological materials & animal
carcasses.
(1) Hot air oven (2) Incinerator (3) Incubator (4)None
19. __________ works under the principle of dry heat sterilization.
(1) Incinerator (2) Incubator (3) Autoclave (4)Shaker
20. ________ works under the principle of moist heat sterilization
(1) Autoclave (2) Hot air oven (3) Incubator (4)Incinerator
21. Endospores are killed at a temperature of ______
(1) 100°C (2) 110°C (3) 120°C (4) 150°C
22. Oxidation of cell constituents and protein denaturation are due to _______ sterilisation
(1) Dry heat (2) Moist heat (3) Both a & b (4) Neither a nor b
23. Which of the following uses flame for keeping away microbes?
(1) Autoclaving (2) Incineration (3) Hot air oven (4)Surface cleaning
24. Log phase culture of microbes can be obtained by _________
(1) Autoclave (2) Incubator (3) Metabolic shaker (4)All
25. The amount of oxygen required by organisms in water under certain standard conditions
is
(1) BOD (2) COD (3) DO (4) All
5X5=25
1.
2.
3.
4.
5.
Explain the principle and applications of an autoclave
Write short notes on hot air oven
Describe the different types of incubator
Write a note on metabolic shaker
Explain incinerator and write its applications
3X8=24
1. Define biosafety cabinet. Explain the types and write the applications
2. Write short notes on a) Hot air oven and b) Incinerator
3. Draw a neat labeled diagram of autoclave and explain its principle and applications.
UNIT III
1) ________ is an instrument which is used to spin substances at high speed.
(1) Centrifuge (2) Spectrophotometer (3) Colorimeter (4) All
2) Centrifuge works under the principle of _______
(1) Weight (2) Gravity (3) Speed (4) All
3) __________centifugation is concerned with separation, isolation and purification of
subcellular structures.
(1) Preparative (2) Analytical (3) Both a & b (4)Neither a nor b
4) ________ centrifugation is used to study purified macromolecules.
(1) Preparative (2) Analytical (3) Both a & b (4)Neither a nor b
5) The centrifugation process is monitored by ______ systems.
(1) Rotor (2) detector (3) Both a & b (4) None
6) _______ are also known as clinical centrifuges.
(1) Desk top (2) High speed (3) Ultra (4) All
7) The maximum speed of small bench centrifuges is _______
(1) 3000 rpm (2) 4000 rpm (3) 5000 rpm (4) 6000 rpm
8) The centrifugal force of desk top centrifuge is _______
(1) 7000 g (2) 90,000 g (3) 500,000 g (4) 1000000 g
9) ________ centrifuge is equipped with refrigeration equipment.
(1) Desk top (2) High speed (3) Ultra (4) All
10) The maximum speed of high speed centrifuges is _______
(1) 25000 rpm (2) 30000 rpm (3) 35000 rpm (4)60000 rpm
11) The centrifugal force of desk top centrifuge is _______
(1) 7000 g (2) 90,000 g (3) 500,000 g (4) 1000000 g
12) The temperature of high speed centrifuge is maintained in the range of ______ degree
(1) 0 to 4 (2) 0 to 5 (3) 0 to 6 (4) 0 to 7
13) ________ centrifuge is used to isolate RBC, yeast cells etc
(1) Desk top (2) High speed (3) Ultra (4) All
14) The subcellular organelles can be isolated by _______ centrifuges.
(1) Desk top (2) High speed (3) Ultra (4) All
15) Ultracentrifuge operate at a speed of ______
(1) 50000 rpm (2) 70000 rpm (3) 75000 rpm (4)80000 rpm
16) The centrifugal force of ultracentrifuge is _______
(1) 7000 g (2) 90,000 g (3) 500,000 g (4) 1000000 g
17) The shaft of ultracentrifuge is made up of ______
(1) Aluminium (2) Titanium (3) Both a & b (4) Either a or b
18) The glass electrode of pH meter contains ________
(1) HCl (2) AgCl (3) Both a & b (4) Either a or b
19) The electrodes of pH meter is kept in beaker filled with _____
(1) Saline (2) distilled water (3) Both a & b (4) Either a or b
20) The pH can be increased by using ______
(1) HCl (2) NaOH (3) Both a &b (4) Either a or b
21) The pH can be decreased by using______
(1) HCl (2) NaOH (3) Both a &b (4) Either a or b
22) Biological samples can be preserved using _______
(1) Incubator (2) Lyophiliser (3) Gene bank (4) All
23) ______ is used for Lyophilisation
(1) Oxygen (2) Ammonia (3) Liquid nitrogen (4) Formalin
24) The temperature of liquid nitrogen is ______ degree Celsius
(1) 196 (2) -196 (3) 198 (4) -198
25) pH can be stabilized by using ______
(1) Acid (2) Alcohol (3) Alkali (4) Buffer
3X5=25
1. Write short notes on low speed centrifuge
2. Draw a neat diagram and explain ultra centrifuge
3. Give the applications of a centrifuge
3X8=24
1. Define centrifuge and explain the different types of centrifuge
2. Write a detailed note on lyophilizer
3. Write short notes on a) Ultra centrifuge b) High speed centrifug
UNIT IV
1) The intensity of colour present in a solution is measured by ______
(1) Colorimeter (2) Spectrometer (3) Nephlometer(4) Photometer
2) The filter used in colorimeter is made up of _____
(1) Quartz (2) Diamond (3) Glass (4) All
3) Bandwidth in a colorimeter can be reduced by increasing the concentration of ______
(1) Dye (2) Sample (3) Both a & b (4) Either a or b
4) Transmission is measured as _______
(1) Fraction (2) Decimal number (3) Percentage (4) All
5) The maximum absorption value of colorimeter is _____
(1) 1 (2) 2 (3) 3 (4) 4
6) The study of absorption and emission of radiation by matter is called ______
(1) Spectroscopy (2) Colorimetry (3) Photometry (4)Nephlometry
7) _____ is a type of energy that is transmitted through space at enormous velocities.
(1) Electromagnetic radiation (2) Optical radiation(3) Magnetic radiation (4) All
8) Electromagnetic radiation is produced by many events occurring at ______ level
(1) Molecular (2) Atomic (3) Nuclear (4) All
9) The wavelength of light used in UV spectrophotometry is ______ nm
(1) 2 – 180 (2) 180 – 400 (3) 400 – 780 (4) All
10) Spectrophotometer works on the principle of ______
(1) Beer’s Law (2) Lambert’s law (3) Beer-Lambert’s law (4) De-Broglie law
11) The monochromators used in spectrophotometry is _______
(1) Prism (2) Diffraction gratings (3) Both a & b (4) Neither a nor b
12) _____ is the source of radiation in spectrophotometry.
(1) Hydrogen lamp (2) Deuterium lamp (3) Both a & b (4) Either a or b
13) _________ is used as source of visible radiation in spectrophotometry.
(1) Hydrogen lamp (2) Deuterium lamp (3) Tungsten filament lamp (4) All
14) Dectectors used in spectrophotometry is _______
(1) Phototubes (2) Photomultiplier tubes (3) Both a & b (4) Either a or b
15) The electric signals produced by detectors is translated with ______
(1) Ammeter (2) Potentiometer (3) Potentiometric recorder (4) All
16) Flame photometry is similar to ________
(1) Colorimetry (2) Spectrophotometry (3)Turbidometry (4) Nephlometry
17) The basic components used for observing emission from flames are ______
(1) Flame (2) Filter (3) Photomultiplier (4) All
18) _______ elements are detected by flame photometer.
(1) Sodium (2) Potassium (3) calcium (4) All
19) Flame photometry is useful in _________ analysis
(1) Qualitative (2) Quantitative (3) Soil (4) All
20)The absorption beam of monochromatic light by atoms in a flame is measured by _______
(1) Emission flame photometry (2) Atomic absorption spectrophotometry (3) Both a & b
(4)Either a or b
21)Turbidimetry is similar to _________
(1) Colorimetry (2) Spectrophotometry (3) Both a & b (4) Nephlometry
22) The scattering of light by non-transparent particles in a solution is measured by _____
(1) Colorimetry (2) Spectrophotometry (3)Turbidometry (4) Nephlometry
23) Turbidimetry can be used on _______ samples
(1) Solid (2) Liquid (3) Gas (4) All
24) Turbidimetry is applicable in _______ analysis
(1) Organic (2) Biochemical (3) Titrations (4) All
25) The amount of radiation scattered by the particles is measured at an angle of _____ degree
(1) 90 (2) 180 (3) 360 (4) 0
5X5=25
1.
2.
3.
4.
5.
Write short notes on principle and applications of calorimeter
Write short notes on principle and applications of turbidometer
Describe UV spectrophotometer with a neat labeled diagram
Describe visible spectrophotometer with a neat labeled diagram
Write short notes on flame photometry
3X8=24
1. Write in detail about the working principle of spectrophotometer and its types
2. Write notes on flame photometer with a neat labeled diagram
3. Describe about a) Calorimeter b) Turbidometer
UNIT V
1) ______ technique is used for separation, identification and estimation of chemical
components.
(1) Colorimetry (2) Turbidimetry (3) Chromatography (4) Electrophoresis
2) Which of the following works on the principle of partition chromatography?
(1) PC (2) TLC (3) Both a & b (4) Either a or b
3) The stationary phase in paper chromatography is _____
(1) Cellulose (2) Silica gel (3) gas (4) All
4) The stationary phase in TLC is _______
(1) Cellulose (2) Silica gel (3) Gas (4) All
5) The mobile phase used in chromatography is ______
(1) BAW (2) Acetone (3) Isopropanol-ammonia water(4) All
6) Lipids are detected by _______
(1) Ninhydrin (2) Folin ciocalteau (3) Rhodamine B (4) CBB
7) Amino acids are detected by ________
(1) Ninhydrin (2) Folin ciocalteau (3) Rhodamine B(4) CBB
8) The matrices commonly used in column chromatography are ______
(1) Dextran (2) Cellulose (3) Polystyrene (4) All
9) Which of the following elution technique is used in column chromatography?
(1) Isocratic (2) Batch (3) Gradient (4) All
10) _______ is a cation exchanger.
(1) CM-Sephadex (2) Polystyrene (3) DEAE sephadex(4) All
11) _____ is an anion exchanger.
(1) CM-sephadex (2) Dowex 50 (3) DEAE sephadex(4) All
12) _______ is used to separate and quantitate volatile compounds in a heated column.
(1) PC (2) TLC (3) CC (4) GC
13) The carrier gases commonly used in GC are _______
(1) Nitrogen (2) Helium (3) Argon (4) All
14) ________ is the migration of charged particles through a solution under electrical field.
(1) Chromatography (2) Electrophoresis (3)Spectrophotometry (4) All
15) Electrophoretic mobility was first developed by ________
(1) Maxam (2) Tiselius (3) Kabat (4) Newton
16) The support material used in electrophoresis is _______
(1) Paper (2) Agarose (3) Polyacrylamide (4) All
17) _______ catalyses the decomposition of persulphate ion in SDS-PAGE
(1) Acrylamide (2) Bisacrylamide (3) TEMED (4) APS
18) DNA is separated by _______ electrophoresis
(1) Agarose (2) SDS PAGE (3) Both a & b (4) Either a or b
19) SDS-PAGE electrophoresis is useful in separation of ______
(1) DNA (2) RNA (3) Proteins (4) Plasmid
20) Dye used in detection of nucleic acids in electrophoresis is ________
(1) Ethidium bromide (2) CBB (3) Sudan Black (4) Iodine
21) Dye used in detection of proteins in electrophoresis is ________
(1) Ethidium bromide (2) CBB (3) Sudan Black (4) Iodine
22) Dye used in detection of lipoprotein in electrophoresis is ________
(1) Ethidium bromide (2) CBB (3) Sudan Black (4) Iodine
23) Dye used in detection of polysaccharide in electrophoresis is ________
(1) Ethidium bromide (2) CBB (3) Sudan Black (4) Iodine
24) Hydrogen bonds in biomolecules are disrupted by ______ in electrophoresis
(1) Urea (2) SDS (3) Beta mercaptoethanol (4)Cellulose
25) Disulphide bridges of proteins are broken by ________
(1) Urea (2) SDS (3) Beta mercaptoethanol (4) Cellulose
5X5=25
1.
2.
3.
4.
5.
Write short notes on paper chromatography
Write short notes on thin layer chromatography
Write a note on gas chromatography
Describe SDS – PAGE electrophoresis
Explain Agarose gel electrophoresis
3X8=24
1. Define Chromatography, explain thin layer HPLC in detail with a diagram
2. Write in detail about the principle and procedure of S
3. Write in detail about the principle and procedure of Agarose gel electrophoresis
Note: Options indicated in green are the correct answers