Download QCPN INVESTIGATOR Name Bruce M. and Jean A

QCPN
INVESTIGATOR
Name
Bruce M. and Jean A. Carlson
Address Department of Anatomy & Cell Biology, University of Michigan, Ann Arbor, MI 48109
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
wing bud ZPA (Zone of Polarizing Activity)
quail (wing bud ZPA)
not known
HH 21-24
mouse
Balb/cJ
female
spleen
11/28/88
134 wedges
I.P.
Freund’s complete
12/20/88
145 wedges
I.P.
Freund’s incomplete
01/20/89
164 wedges
I.P.
none
01/23/89
mouse
P3X63 - Ag 8.653
IgG1, kappa light chain
immunofluorescence: thin perinuclear ring seen in all Quail cells, with possible exception of
embryonic blood cells, HH 18 through 27 (not tested before 18 or after 27). does not stain
the Chick (hence the C) stages tested (18 through 27).
ANTIGEN
Chemical properties
not known at this time
Molecular weight
not known at this time
Characterization
not known at this time
Immunoprecipitation
Immunoblotting
Purification
Amino acid sequence analysis
Functional effects
Immunohistochemistry
PUBLICATIONS :
Selleck, A.J., and Bronner-Fraser, M. (1995). Origins of the avian neural crest: the role of neural plate-epidermal
interactions.Development 121, 525-538.
Liem Jr., K.F., Tremml, G., Roelink, H., and Jessell, T.M. (1995). Dorsal differentiation of neural plate cells induced by BMPmediated signals from epidermal ectoderm. Cell 82, 969-979.
Yablonka-Reuveni, Z., Schwartz, S.M., and Christ, B. (1995). Development of chicken aortic smooth muscle: expression of
cytoskeletal and basement membrane proteins defines two distinct cell phenotypes emerging from a common lineage. Cell. &
Mol. Biol. Res. 41(4), 241-249.
(continued)
QCPN (continued)
Kontges, G., and Lumsden, A. (1996). Rhombencephalic neural crest segmentation is preserved throughout craniofacial ontogeny.
Development 122, 3229-3242.
Schneider, R.A. (1999). Neural crest can form cartilages normally derived from mesoderm during development of the avian head
skeleton. Dev. Biol. 208, 441-455.
Manner, J. (1999). Does the subepicardial mesenchyme contribute myocardioblasts to the myocardium of the chick embryo heart? A
quail-chick chimera study tracing the fate of the epicardial primordium. Anat. Rec. 255, 212-226.
Baker, C.V.H., Stark, M.R., Marcelle, C., and Bronner-Fraser, M. (1999). Competence, specification and induction of Pax-3 in the
trigeminal placode. Development 126, 147-156.
Groves, A.K., and Bronner-Fraser, M. (2000). Competence, specification and commitment in otic placode induction. Development
127, 3489-3499.
Schneider, R.A., Hu, D., Rubenstein, J.L.R., Maden, M., and Helms, J.A. (2001). Local retinoid signaling coordinates forebrain and
facial morphogenesis by maintaining FGF8 and SHH. Development 128, 2755-2767.
Matsushita, S., Ishii, Y., Scotting, P.J., Kuroiwa, A., and Yasugi, S. (2002). Pre-gut endoderm of chick embryos is regionalized by
1.5 days of development. Dev. Dyn. 223, 33-47.
Schneider, R.A., and Helms, J.A. (2003). The cellular and molecular origins of beak morphology. Science 299, 565-568.
Navarro, M., DeRuiter, M.C., Carretero, A., and Ruberte, J. (2003). Microvascular assembly and cell invasion in chick mesonephros
grafted onto chorioallantoic membrane. J. Anat. 202, 213-225.
Gumati, M.K., Magyar, A., Nagy, N., Kurucz, E., Felfoldi, B., and Olah, I. (2003). Extracellular matrix of different composition
supports the various splenic compartments of guinea fowl (Numida meleagris). Cell Tissue Res. 312, 333-343.
Lear, P.V., Jayanthi, N.V., Teague, W.J., and Johnson, P.R. (2004). Foregut mesenchyme contributes cells to islets during pancreatic
development in a 3-dimensional avian model. Organogenesis 1(2), 45-51.
Teague, W.J., Jayanthi, N.V., Lear, P.V., and Johnson, P.R. (2005). Foregut mesenchyme contributes cells to pancreatic acini during
embryonic development in a chick-quail chimera model. Pediatr. Surg. Int. 21(3), 138-142.
Eames, B.F., and Schneider, R.A. (2005). Quail-duck chimeras reveal spatiotemporal plasticity in molecular and histogenic programs
of cranial feather development. Development 132, 1499-1509.
Nagy, N., Biro, E., Takacs, A., Polos, M., Magyar, A., and Olah, I. (2005). Peripheral blood fibrocytes contribute to the formation of
the avian spleen. Dev. Dyn. 232, 55-66.
Nagy, N., and Goldstein, A.M. (2006). Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric
nervous system. Dev. Biol. 293, 203-217.
Nagy, N., and Goldstein, A.M. (2006). Intestinal coelomic transplants: a novel method for stydying enteric nervous system
development. Cell Tissue Res. 326, 43-55.
Nagy, N., Brewer, K.C., Mwizerwa, O., and Goldstein, A.M. (2007). Pelvic plexus contributes ganglion cells to the hindgut enteric
nervous system. Dev. Dyn. 236, 73-83.
Goldstein, A.M., and Nagy, N. (2008). A bird’s eye view of enteric nervous system development: lessons from the avian embryo.
Pediatr. Res. 64, 326-333.
Merrill, A.E., Eames, B.F., Weston, S.J., Heath, T., and Schneider, R.A. (2008). Mesenchyme-dependent BMP signaling directs the
timing of mandibular osteogenesis. Development 135, 1223-1234.
Matsushita, S., Urase, K., Komatsu, A., Scotting, P.J., Kuroiwa, A., and Yasugi, S. (2008). Foregut endoderm is specified early in
avian development through signal(s) emanating from Hensen’s node or its derivatives. Mech. Dev. 125, 377-395.
Nagy, N., and Olah, I. (2010). Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of
Fabricius. Development 137, 3019-3023.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242