Download URK20BT1034. BAT Lab Exp.3

EXPT.
NAME:
NO.
Estimation of, Polyphenols, by
by
Colorimetric
Method
Aim
To estimate
present
method.
în
the amount
qiven
Sample
Equipments Test tubes
pipettes
Page No.:
YOUv
DA1/0LJa2
ot
by
by
polyphenol
Colorimetri
colorimeter
and
Cuvettes.
Reaqents :
0-1 N Folin's Ciocaltaeucagent 1 in 1a dtlution
l o % 5odium
Palyphena
carbonate
standard9uaiacol 0.12
20 mqper ml ot quaiacol standard in
0
ethanol.
:5mlot stock standard
sWorkinq StandardL:
is
diluted
to loo mlConcentration
Imglml
Principle 3
The hydroxsl qraups are 0xidized by he
phosphor tungsic acid
reducin4 agent
Eolin's
Ciocataeu
reagent
which Can be me asured
to
product.
blue Colarproduct,
at 7o0 nm.
Teacher's Signature
EXPT.
Page No.
NAME:
NO.
YouvA
Date
Proceduxe
Pipete out
standard with
O.2 to 1 ml ot workinq
which Corre sp onds
1 m
mq respechvely.
and
0.5 ml
0.6, 0.8
to
To
all
0.2, 0.4,
test
reaqenot
ofFolin Cioaltaeu
The volume of alltest ubes
i s made upto 3.5 ml using
disilled water
Then added 5m
of LO Sodium carbanate
Then
tubes
is added
the sest tube are
for a n hour
Added
water and
measured
All
allowed
to
6m o
Hhe
the
Stand
distilled
absorbance
at 100 nm-
Preparation
o
lqot
exracted
3 ml
with
of H
unil
the
Then
6.S
dest Sample :
Hower5ample is_colle.cfe.d
experlment-
1
m
of 70Jethano
acetonitrile
extract
Sample
and
or
and
and
methanol
Lat s 7o is
Color leSS.
aken tor the
teated a s above
was
Teacher's Signature:
Qbservahian
Concentrathon
Sr.No Par Hculars
1.
Standard
B
solution (mlo.0
2. Concentration (ug)
3
4
Test sample
Disilled
S
0.2
wakr (ml)3.S33
6
0
(ml)
Sodium
Add
6ml
S3
Ss
S4
0-4 . 6
T
1
400
600
&00
31
2-9
2-7
Carbonate
the
of
of
9. Optical density
minture
disilled
2.S
3
5
Cmd)
Incubate
stand 1mq /ml
wor Finq
(ml)
Folin ciocalteau reagent
g.
S
200
5
7.
of
for qn
our
water to
O.9
0-14
all
at
room
fest
0.23 0.3o
tem p.
tubes.
0
35 0. 13
EXPT.
NAME:
NO.
Page NO.
YoUVA
Date
Calaulaions
From he
0.18
9raph
est Sqmple
OD of
L0 u
Tes+
of
0fpolyphenol
Contains
here tore
490x
o0
O S
corres pon ds
to
ie, 0:5 ml of
1gof polyp heno
o0 m
ot
Hower exiract contain8
2000ug
98 ma.
ReSult
The
106 m
amountot poly pheno
_0f
92000U
qiven
sample
present in
is
9R m
-x-
Teacher's Signature-