Download CollaboraƟon / Licensing Opportunity HistaƟn

CollaboraƟon / Licensing Opportunity
HistaƟn-1 improving integraƟon of dental implants
x Improved soŌ Ɵssue integraƟon and osseointegraƟon of dental implants based on a
cellular response to histaƟn-1
x HistaƟn-1 can be combined with exisƟng techniques to improve integraƟon of implants
x Good biocompaƟbility: histaƟn-1 is naturally present in the human body
Hista n-1 | so
ssue integra on | osseointegra on
Background
In Vitro Data
Hista ns are pep des exclusively present in the saliva
of higher primates. They are secreted by the salivary
glands and play an important role in protec on of the
mouth. Recently we found that one of the hista ns,
hista n-1 (Hst1), improves cell-substrate
adhesion as well as cell-cell adhesion, sugges ng a
role in maintenance of the epithelial architecture and
barrier func on. The effect of Hst1 on cell-substrate
adhesion is very pronounced, including substrates
like glass and tanium.
Scien sts from the Academic Medical Center (AMC)
and the Academic Centre for Den stry Amsterdam
(ACTA) – one of the top 5 dental research ins tutes
in the world –showed that Hst1 causes a significant,
more than twofold increase in the ability of human
adherent cells (oral and non-oral) to a ach and
spread, even in condi ons that impair cell spreading.
Other tested hista ns do not s mulate cell spreading,
indica ng a specific effect of Hst1. Cells treated with
Hst1 not only a ach more rapidly, but are also more
firmly a ached to a substrate.
Titanium is the most commonly used material for
dental implants. We have shown improved a achment of several human gingival cell types, treated
with Hst1, on tanium with a smooth surface, as well
as osteoblast on an SLA-treated tanium surface.
Furthermore, we found that canine cells respond
in a similar way to Hst1, providing opportuni es for
animal tes ng.
The Technology
Infec on is a common cause for implant failure due
to poor adhesion of the implant to so ssues and
bone.
Hst1 improves a achment of both so ssue cells
and bone cells to dental implants by promo ng
cell-substrate adhesion, important to seal off the
lower part of an implant and thereby prevents
infec ons. Moreover, Hst1 may shorten the period
between placing the implant and loading it with
a replacement tooth. Conven onal techniques to
improve so ssue integra on and osseointegra on
are based on modifica ons of implant design, surface
topography and chemistry. An advantage of the method described here is that it can be combined with
existent techniques. Toxic side-effects are not to be
expected, since Hst1 is a pep de naturally present in
the human body, and we found no nega ve effect of
Hst1 on cell survival.
Hist1 could be administered by injec on to give a
burst release or, alterna vely, techniques are available to have a prolonged, slow release of Hst1.
ApplicaƟons
Hst1 can be used in combina on with exis ng techniques to improve integra on of dental implants. Hst1
can also be applied to implants or materials placed in
other sites in the human body.
What are we looking for?
Licensing and collabora on partner for this technique
to extend the exis ng data set with in vivo data. Our
lab has all the exper se and techniques to form a
frui ul collabora on.
Inventors
Dr. Jan Stap – Department of Medical Biology, AMC
Irene van Dijk, MSc – Department of Medical Biology,
AMC & Department of Oral Biochemistry, ACTA
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Figure 1: human gingival epithelial cells, fibroblasts and osteoblasts aƩach beƩer to Ɵtanium in the presence of histaƟn-1.
(C = control, Hst1 = HistaƟn-1, C pep = Control pepƟde). Right hand side: microscopy images of epithelial cells on Ɵtanium,
made visible with an orange fluorescent dye.
Figure 2: human gingival epithelial cells, fibroblasts and osteoblasts spread beƩer on Ɵtanium in the presence of histaƟn-1.
(C = control, Hst1 = HistaƟn-1, C pep = Control pepƟde). Below: microscopy images of epithelial cells on Ɵtanium, made
visible with an orange fluorescent dye. White arrows indicate spreading cells.
Key PublicaƟons
I.A. van Dijk et al. Hista n 1 enhances cell adhesion
to tanium in an implant integra on model. Journal
of Dental Research, online publica on Dec 2016
I.A. van Dijk et al. Hista n-1, a his dine-rich pep de
in human saliva, promotes cell-substrate and cell-cell
adhesion. FASEB Journal, Aug 2015; 29(8):3124-32.
For more informaƟon about collaboraƟon
opportuniƟes
visit the IXA website: h p://www.ixa.nl/en/for-companies.html
or contact our office: S.J. Stembert
[email protected] or Tel: +31 20 5668022
Contact x Sanne Stembert x +31 20 566 8022 x [email protected] x www.ixa.nl
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