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DAZL is upregulated in 2i culture conditions FACS analysis showing Dazl‐GFP expression in serum‐ or N2B27‐cultured ESCs in LIF (upper panel) and 10 days in 2i + LIF (lower panel).Expression profiles of germ‐cell reporter ESCs Dazl‐GFP, Nanog‐GFP, and Stella‐GFP at day 0, 3, and 10 after 2i induction analyzed by fluorescent microscopy.mESCs were profiled for mRNA (RNA) and protein (PRO) expression at day 3 and 12 after addition of 2i inhibitors to the culture media. Maaike Welling et al. EMBO Rep. 2015;16:791-802 © as stated in the article, figure or figure legend
