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MDH Flow Chart for Rule-out and Referral of Bacillus anthracis Public Health Laboratory - Emergency Preparedness and Response Unit 601 Robert St. N. PO Box 64899, St. Paul, MN 55164 651-201-5200 http://www.health.state.mn.us/divs/phl/labep/index.html Colony Morphology on SBA • • • Large rough “ground glass” colony Irregular shaped edges, comma like projections “Sticky”—adheres to the agar • • Large gram positive bacilli 1 -1.5 x 3 – 5 µm Gram Stain Morphology Large gram positive rod Bacteria not a large gram positive rod Hemolysis Hemolytic Not B. anthracis Not B. anthracis Non-hemolytic Catalase Positive Negative Not B. anthracis Motility Negative Positive Not B. anthracis REFER ISOLATE: Cannot Rule Out B. anthracis Call MDH-PHL at 651-201-5073 after hours number: 877-676-5414