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FRAUNHOFER INSTITUTE FOR CELL THERAPY AND IMMUNOLOGY IZI LAB-ON-CHIP FOR RAPID DIAGNOSIS OF INFECTIOUS DISEASES Currently a reliable diagnosis of complex Unique feature and life-threatening infectious diseases like sepsis is only possible using elaborate The MinoLab lab-on-chip device is based and time-consuming methods involving on magnetic microparticles, which combine different functionalities: Ŷ Allow effective capturing of bacteria- or specialists. Together with different partners from Industry and with support Fraunhofer Institute for of the German Ministry for Education Cell Therapy and Immunology IZI and Research (BMBF), the Fraunhofer IZI develops an innovative system for rapid, Perlickstraße 1 easy-to-conduct and inexpensive on-site 04103 Leipzig infection diagnostics called MinoLab. Germany fungal cells out of patients samples Ŷ Enable electromagnetic transport of attached target cells through the labon-chip device Ŷ transport Ŷ Microparticles develop a detectable signal on the surface of a magneto- Contact resistive DNA chip Dr. Dirk Kuhlmeier Department of Diagnostics Method Nanotechnology Unit Phone +49 341 35536-9312 The core of this novel device is the nano- [email protected] meter- to micronmeter-sized magnetic particle that can be functionalized www.izi.fraunhofer.de Technology principle separation magneto sensor Cell isolation DNA isolation particles and all necessary reagents are amplification Potential applications Nanotechnology Unit card format. In case of an on-site examina- Sepsis, blood poisoning, is one important In the following areas partners from tion, a sample, e. g. blood, saliva or urine, example for a potential application. About is taken from the patient and applied onto half of the approximately 154,000 annual the card. Upon lysis of the target cells, the cases of sepsis in Germany must be treated from experienced and reliable people: Ŷ Development of integrated magnetic particles bind to the respective in intensive care and still more than 40 target molecules contained in the sample percent of those cases treated result in the and are transported in a fully automated lab-on-chip devices Ŷ Assay development Ŷ Rare cell diagnostic manner through the individual reaction to the fact that sepsis can be caused by Ŷ Nucleic acid isolation and cavities via magnetic forces. At the end of dozens of different pathogens. Moreover, the process chain, detection is conducted individual classes of these pathogens have Ŷ Functionalization of surfaces in by means of highly sensitive magnetic already developed a resistance to certain sensor technology – the signals are therapeutic agents. The time between digitalized and a fully electric read-out blood sample collection and obtaining a the nanometer scale Ŷ Sensor development Ŷ Test strip development is performed. diagnostic result still amounts to at least Ŷ DNA origami techniques for drug stored on a single-use cartridge in check delivery or systematic surface eight hours. By integration of all process steps from sample preparation to detection on the innovative diagnostic platform, we are aiming for a time-to-result of less than two hours. In addition to medical applications such as proteomic, genomic and microbiological tests, the future areas of application also comprise environmental analyses or civil protection measures.