Download Jatropha genotyping In Gh Pu QR In Gh Pu QR 13 primer pairs

Getting Biodiesel From
Jathropa
The jatropha seeds,
commonly known as
the physic nuts, contain
up to 40% oil. The
jatropha oil can be
used directly as biofuel
or can be converted
into biodiesel for a
more efficient
performance. When
raw or mixed jatropha
oil is directly used in an
automobile or even
plane engines
Jatropha genotyping
In Gh Pu QR
In Gh Pu QR
• 13 primer pairs selected for use in
further studies
• These reveal 69/453 polymorphic bands
(15.2%)
• Results indicate very little variation
between accessions from India, Ghana,
Tanzania & Madagascar
Considerations for using DNA
markers in plant breeding
• Technical methodology
– simple or complicated?
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Reliability
Degree of polymorphism
DNA quality and quantity required
Cost**
Available resources
– Equipment, technical expertise
Genetic Markers
• A gene or DNA sequence having a known location on
a chromosome and associated with a particular gene
or trait
• Advantages of genetic markers:
– Profile of genetic markers will define a genotype
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Uniform at all tissues at all ages of the organism
allows early detection
developmentally stable and not affected by the environment
highly specific pattern can be developed for each individual
• Utilities:
– For biodiversity study
– To choose right parents for cross pollination
– Marker assisted breeding
– To differentiate plant varieties
– Quality control of plantation
RFLP
Overview of
‘marker
genotyping’
(1) LEAF TISSUE
SAMPLING
(2) DNA EXTRACTION
(3) PCR
(4) GEL ELECTROPHORESIS
(5) MARKER ANALYSIS