Download PRELIMINARY APPROACH OF THE BIOLOGICAL RESPONSE OF

9º Congresso Latino-Americano de Orgãos Artificiais e Biomateriais
13º Congresso da Sociedade Latino Americana de Biomateriais, Orgãos Artificiais e Engenharia de Tecidos - SLABO
24 a 27 de Agosto de 2016, Foz do Iguaçu, PR
PRELIMINARY APPROACH OF THE BIOLOGICAL RESPONSE OF OSTEOBLASTIC AND
OSTEOCLASTIC CELLS TO ANODISED ZIRCONIUM IMPLANTS
Katunar, M.R.(1); Gomez-sanchez, A.(1); Santos-coquillat, A.(2); Martinez-campos, E.(2);
Civantos,
A.(3);
Ramos,
V.(2);
Ceré,
S.M.(1);
(1) INTEMA; (2) IEB; (3) CSIC;
Palavra chave: osseointegration-osteoblast-osteoclast-zirconium -anodisation
Resumo:
Most metals used as cementless implants undergo some k ind of surface modification before
clinical insertion. Modifications are performed to promote biological reactions at the interface that
lead to bone formation. The material surface modifications and implant topography are critical to
the success of bone-anchored implants. Zirconium (Zr) is an ideal metal for intra-osseous
implants due to its favorable resistance to corrosion, osseointegration and low metal ions
migration. The aim of this study is focused on the first events that take place on anodised Zr
implants employing an in vitro approach. The cytocompatibility of the material and its surfac e
treatment was evaluated. Cell adhesion and proliferation were studied in the preosteoblastic
MC3T3 cell line 72 and 120 hours after seeding. Our results highlight that anodisation treatment
at 60V allowed stronger cell attachment promoting cell proliferation at early stages. After the
induction of cell differentiation the cell morphology was assessed through an Actin staining
(cytoskeleton) and Hoechst staining (cell nuclei). The study showed an interesting relationship
between the differentation process and material surface especially for the samples treated at 60V.
In previous studies we observed that some remodeling events take place in the interface between
the material and the new bone in vivo, so we evaluated if the surface would influence in the
differentiation of the macrophage mouse cell line RAW 264.7 towards osteoclasts cells in
presence of RANKL. We observed that the surface treatment not only accelerated the fusion of
macrophages, forming osteoclasts but also stimulated a particular cytoskeleton alignment. In
conclusion, it can be speculated that, the anodisation treatment over Zr at 60V would stimulate
the new bone formation and also the remodeling events may be beneficial for future orthopedic
applications.
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