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Endocrine Disrupting Chemical Effects on Embryonic Development Carthage College. Biology – Fall ’08 Sara May and Kristiena Escobar Advisor: Dr. Dan Choffnes The purpose of this ongoing project is to efficiently determine the effects of endocrine disrupting chemicals (EDC) on the embryonic development of Xenopus laevis. • Two different chemicals were used as controls to provide guides of what embryos/tadpoles will look like in normal and toxic environments. • The negative control was FETAX (Frog Embryo Teratogenesis Assay Xenopus). A developed embryo is featured in the photograph on the left, Fig. 1 (A). • The positive control was 6-aminonicotinamide, which is a known EDC. A developed embryo is featured in the photograph on the right, Fig. 1 (B). Fig. 1 (A) Embryo development in control solution Fig. 1 (B) Embryo development in solution containing 6aminonicotinamide • Human Chorionic Gonadotropin was injected into the dorsal lymph sac twice within 72 hours of each other to allow the female to produce eggs and the male to fertilize them. • Each control had three replications of approximately 25 developing embryos in 25 mL of the chemical • Embryos were staged and malformations were noted. • Further research will be done with specific EDCs that are prevalent in local water systems.