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Download Supplement: Sequence analysis of GMR-dRetMEN2B
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Supplemental Table 3A: Sequence analysis of GMR-dRetMEN2B P element modifiers GMRGenome Gene/allele dRetMEN2B Scaffold comments on P insertion site dMi-2S005504 ME AE003515 in 1st intron of dMi-2 l(3)S003704 WE AE003712 in intron of gish l(3)S023708 ME AE003773 in coding exon on Fer2LCH l(3)S023549 WE l(3)S026421 WE AE003527 in 1st exon of argos (minus strand) and exon of CG33158 (plus strand) AE003591 in 1st exon of DNA primase l(3)S046604 WE AE003686 in 1st intron of jumeau bonS048706 WE AE003731 in 1st intron of bonus dMi-2S047526 ME AE003515 in 1st intron of dMi-2 l(3)S066607 WE AE003565 inserted 75 bp upstream of DnaJ-1 l(3)S063512 WE AE003554 inserted 19 bp upstream of hairy l(3)S056113 WE AE003683 maps to different sites within AE003683. There may be 2 inserts, one in 1st intron of Dh, the other 92 bp upstream of Crc, or a single P that induced a 10.4 kb deletion to remove Scm, SpdS, the 1st exon of Dh l(3)S090114 WE AE003693 maps to different sites in AE003693. There may be 2 inserts, one 2.2 kb upstream of aurora, the other 1.6 kb upstream of Hsp70Aa, or P may have induced a 2.5 kb deletion to remove Hsp70Ab. dMi-2S147412 WE AE003515 in intron of dMi-2 l(3)S146006 WS AE003514 inserted between CG17149 and CG13813 l(3)S130910 WE AE003733 P inserted within the 1st exon of Mvl and 1.2 kb donwstream of cortactin (minus strand); 4.7 kb upstream of AnnIX (plus strand) The P element line from which flanking genomic sequence was obtained is noted. Sequences were matched to full genomic sequence and the insertion site was mapped relative to known and predicted genes. The genomic scaffold segment is indicated. Supplemental Table 3B: Sequence analysis of GMR-dRetMEN2B P element modifiers, sequence from BDGP GMRGene/allele dRetMEN2B genome scaffold comments on P insertion site msnj1E2 ME AE003475 in 1st exon of misshapen dCskj1D8 WE AE003692 in 1st intron of dCsk hhrJ413 WE AE003741 inserted 974 bp downstream of hh coding region crbj1B5 WE AE003747 inserted 260 bp upstream of crb l(3)j2D5 ME pxk08316 WS AE003457 in 1st intron of plexus Sk09538 WS AE003587 in 1st exon of S kismetk10237 WS AE003590 in 1st intron of long kismet transcript drkk13809 WS AE003818 inserted 753 bp upstream of drk ebik16213 SS AE003589 inserted 122 bp upstream of ebi Ras85D06677 WS AE003683 in 2nd exon of Ras85D l(3)06803 WS AE003476 in 1st intron of CG2162 l(3)06906 WS AE003744 in 1st exon of CG13822 l(3)j5B6 WE AE003554 in 1st exon of CG32030 neurj6B12 WE AE003681 in 1st intron of neuralized Ppi-87Bj6E7 WE AE003695 in 1st exon of Pp1-87B scribj7B3 WE AE003756 inserted 2.8 bp upstream of scrib Sin3A08269 WE AE003821 in 1st intron of Sin3A unknown inserted into YOYO repeat drk10626 SS AE003818 in 1st intron/alternate exon of drk The P from which flanking genomic sequence was obtained is noted. Sequences were matched to full genomic sequence and insertion sites were mapped. The genomic scaffold segment is indicated. All sequence data shown for the Bloomington lines was generated by the Berkeley Drosophila Genome Project and deposited in Genbank.
