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Hivebench Whole-cell recording of RPeD1 voltage-gated Na+ current Created by NancyDong General Author: NancyDong Laboratory: Date: 09-07-2016 Abstract What is this protocol useful for? Materials • Reagent 1 • Reagent 2 Equipment • Equipment 1 • Equipment 2 Procedure • low [Ca2+]o bath • high Cs pipette solution. • The INa was abolished in Na+-free solution and blocked by Na+-channel-specific blocker, TTX, in a dose-dependent manner. • The TTX dose-response curve showed a mean IC50 value of 23.7 ± 2.1 µM (n=7). • The threshold of activation for the current is in the range of -40 mV to -30 mV with the peak current at 1.8 ± 5.7 mV (n=7). Recipes List recipes for solutions and medium. References Link scientific articles.
