Download Cell growth comparison of Porvair Sciences tissue culture

Tissue Culture Application Note 1
Cell growth comparison of Porvair
Sciences tissue culture plate against a
market leading competitor
Margaret Clatworthy Sean Griffiths, Department of Cell Biology, Swansea University
Aim
The experimental aim was to investigate characteristics of established cell lines in culture
using PORVAIR SCIENCES tissue culture vessels and comparing this to another leading
manufacturer. Quality assurance of PORVAIR SCIENCES products is warranted as these have
the potential to provide a viable and economic alternative to the market leader.
Method
Two established cell lines were use in this study. The fibroblastic Chinese Hamster Ovary
(CHO) and endometrial epithelial HEC-1A cell lines were selected on the basis of their
contrasting morphology. The cell lines were seeded at an initial seeding density of 1x10 5
cells/ml in order to achieve a sub-confluent population at the time of analysis. The
incubation time was 24hrs @ 370C and 5% C02. The cell lines were studied in the exponential
growth phase using the MTS assay and phase contrast light microscopy. The MTT assay and
the MTS assay are colorimetric assays for measuring the activity of enzymes that reduce MTT
or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple colour. A main application
allows assessing the viability (cell counting) and the proliferation of cells (cell culture assays).
Results
The cells were initially seeded at
the same density so a cell count
was able to determine the relative
success of the cells on each plate.
The results are an average of 4
wells. The results suggest that the
PORVAIR SCIENCES plate provides
a
slightly
more
favourable
environment for cell growth than
the competitor plate (fig.1) in both
the HEC-1A (0.79-0.75) and CHO (0.64-0.52) cell lines. The difference in absorbance values
(relating to cell count) between the plates is slight.
Figure 2. The HEC1A and CHO cell lines photographed using a light microscope
Light microscope images (fig.2) show similar populations of HEC1A and CHO cells on the
PORVAIR SCIENCES and Competitor 24 well plates and 96 well plates after 24hrs incubation.
For more information about Porvair Sciences TC treated plastic labware, or to request a
sample,, please call Katie Bithell on +44 1978 666240 or e-mail
[email protected] or visit our website www.tissuecultureplastics.com