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DANAGENE TURBO Tissues & Cells Kit This kit allows the purification of PCR-ready genomic DNA from tissue and animal cell samples, mouse tails, hair shafts and bacteria in just 2 steps taking only 15 minutes. Apart for the reagents for the extraction process, the kit includes a ready to use HOT STAR Polymerase 2X that allows the amplification of any fragment from the extract in an easy way as the customer only has to add water and primers. It requires 10 minutes activation step at 95ºC in order to remove nonspecific products, such as primer-dimer. It also contains a red dye which allows an easy visualization and direct loading onto a gel avoiding the need of mixing with a loading buffer. SAMPLE LYSIS SOLUTION 15 MINUTES AT 95ºC Features: It allows a fast isolation of genomic DNA ready for PCR in just 15 minutes. It allows to process a high number of samples for genotyping, SNP analysis, etc. Protocols for tissue and animal cells samples, mouse tail, hair and bacteria are supplied.. It includes all necessary reagents for doing the total process, even a ready to use HOT STAR Polymerase.. It can be automated. NEUTRALIZATION SOLUTION PCR REACTION DANAGENE TURBO Tissues & Cells Kit Ref.1104 For 100 extractions and amplifications from tissues, mouse tail o cells PCR from isolated and amplified DNA using the DANAGENE TURBO Tissues & Cells Kit DNA was isolated from mouse tails and an amplification of the Myf-5 gene was carried out giving a 200 bp for normal mice and mutant mice who take a sequence LoxP 275pb. 1 – Molecular weight marker. 2 – Normal mouse. 3 – Heterozigous mouse. 4 – Heterozigous mouse. 5 – Negative contro.l