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Transcript
PHSI 3006 ANALYSIS OF CELL PROLIFERATION IN CULTURE Lab report pro forma This lab report forms 1 assessment of the course. It is to be handed into the Physiology Admin office by 4.30 pm of the day stipulated for your lab stream (as detailed below). Stream 1: Wed 30 May Stream 2: Wed 6 Jun Stream 3: Wed 13 Jun This assessment is worth 10% of your final mark. Failure to submit your report on time without an impairment form will result in a mark deduction of 10% per day of the final mark. The report is to be completed on the following sheets. Answer all questions. Failure to follow the pro forma will result in a zero mark allocation. A model report will be posted on WebCT after the final deadline has passed. This structured report form is designed to test your ability to present and interpret data in a concise and accurate manner. Questions are designed to help your understanding of key issues that are raised by the experiments undertaken. The experiments should demonstrate some of the rules that govern the normal “social” behaviour of cells. Tips for good marks Follow the instructions carefully. Concise means just that. Space provided is adequate. Taking more space will not gain, but decrease, your score. All data should be given as means (± s.d.). Failure to do so will decrease your marks. All graphs should have appropriately labelled axes. Continue to the next page. Experiment 1: Cell proliferation of a somatic cell Provide a graph of your data below. Your figure should have a concise, descriptive accurate legend. In it you should describe concisely the effect of time on cell number. What did you expect to see and why? Calculate the “doubling time” (an estimate of the time taken for completion of 1 cell cycle) of the cells in your culture. Experiment 2: Growth requirements of cell proliferation Provide 2 graphs of your data below. Your figures should have concise, descriptive accurate legends. In them you should describe concisely the effect of time with cell number. Think carefully how best to present your data (it should be in the most informative and concise manner) What did you expect to see and why? Serum removal from media is a common approach to synchronising cells to the same stage of the cell cycle. What factors might be present and which part of the cell cycle is most likely affected? Experiment 3: Anchorage dependent vs independent growth What feature of growing CH-1 cells was obviously different from the normal somatic and embryonic cell line? On reaching confluency, what do you predict would happen to cells in cultures of F9 and C2C12 somatic cells? Your answer should demonstrate your reasoning. THANK YOU.