Download Type of communication, oral or poster (Times New Roman 12)

Type of communication, oral or poster (Times New Roman 12)
Title (Times New Roman 14, bold)
Presenting author Family Name, A.1, Family Name, B.2, […] Family Name, C.3 (Times
New Roman 12)
1
Affiliation 1
Affiliation 2
3
Affiliation 3 (Times New Roman 12, italics)
2
Abstract text, maximum two A4 sheets (Times New Roman 12)
Example:
Poster communication
A differential expression proteomic approach to study the responses of
holm oak (Quercus ilex L.) to moderate drought stress
Echevarría-Zomeño, S.1, Navarro-Cerrillo, R.M.2, Ariza, D.2, Jorge, I.2, Lenz Ch.3, Jorrín, J. 1
1
Plant Proteomics-Agricultural and Plant Biochemistry Research Group, Dept. of Biochemistry
and Molecular Biology, University of Córdoba, Córdoba, Spain
2
Department of Forestry Engineering, ETSIAM, University of Córdoba, Córdoba, Spain.
3
Applied Biosystems Deutschland, Darmstadt, Germany
To prevent seedling mortality in Quercus ilex plantations, the application of an effective
reforestation programme is necessary. To fight drought stress, the main cause of
mortality in holm oak plantation, a selection of stress-tolerant genotypes, assisted by a
better understanding of the mechanisms responsible for the drought stress, is needed. In
order to find these mechanisms thought the proteins implicated, a differential expression
proteomic approach has been used. Q. ilex seedlings from the provenance Región
Extremadurense-Sierra Morena Occidental (Qi11e) were subjected to three different
treatments in a 14-day long experiment: i) well-watered, control plants, ii) water
withheld throughout the whole period, and, iii) water withheld for 7 days plus a
recovery phase, with irrigation, for an additional 7-day period. Measurements of water
potential, shoot relative water content (RWC) and quantum yield efficiency of
Photosystem II were taken to evaluate seedlings water stress status. Protein expression
changes in leaves in response to water stress were studied by two-dimensional
electrophoresis (2-DE). Both qualitative and quantitative differences in the protein
expression pattern were found between treatments. The differentially-expressed proteins
were identified by enzymatic digestion, LC/MS/MS analysis, de novo sequencing and
sequence similarity searching. The proteins identified belonged to the following groups:
photosynthesis, carbohydrate and nitrogen metabolism enzymes, and stress-related
proteins. Results are interpreted in terms of a reduced photosynthesis and metabolic
activity under drought conditions.