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Annexure ‘AAB-CD-01’
Course Title: Essentials of Molecular Biology
Course Code: to be decided later
Credit Units: 4
Level: PG
L
T
P/
S
SW/F
W
TOTAL
CREDIT
UNITS
3
0
2
0
4
#
Course Title
1
Course Objectives:
This course will establish the foundations of biological systems at molecular level and the methods to exploit them to further the
understanding of immunology and virology
Prerequisites: Graduation in Life Sciences
Student Learning Outcomes:
2
3
4
5
6
7
At the end of this course, the students will be able to:
 Define and analyze the structural features of genetic materials
 Describe the prokaryotic and eukaryotic gene expression
 Describe mobile genetic elements
 Define enzymes that are used to exploit cells and organisms
Module I DNA & Protein Structure and Function
Genetic material: Structure & Replication of dsDNA, ssDNA, dsRNA, ssRNA
Proteins: Amino acids, primary, secondary and tertiary structures, mRNA synthesis & processing, genetic code, protein
localization
Module II Chromosomes
Chromosomes, chromatin and function, Mutations and their consequences, Repair of DNA, Recombination, Transposons &
retroposons
Module III Prokaryotic gene expression
Prokaryotic gene expression, Polymerase–promoter interactions, Control of transcription initiation and termination
Module IV Eukaryotic gene expression
Weightage
20%Weightage
20%Weightage
20%Weightage
20%Weightage
8
9
10
11
Eukaryotic gene expression – Regulatory elements, Splicing and RNA processing
Module V Working with DNA
Growth and maintenance of bacterial cultures, bacteriophages plasmids, Growth and maintenance of animal cells and
viruses
Enzymes used in genetic engineering experiments, DNA
polymerases, ligase, reverse transcriptase, restriction
endonucleases and other enzymes
Oligonucleotides synthesis & purification, PCR
Lab/ Practicals details
 Preparation of competent bacterial cell
 Ligation
 Transformation of bacteria with a plasmid
 Screening of transformants
Pedagogy for Course Delivery:
Interactive classroom teaching from text books, published reviews and articles along with the use of multimedia resource
Assessment/ Examination Scheme:
Theory L/T (%)
Lab/Practical/Studio (%)
Total
75
25
100
Theory Assessment (L&T):
Continuous Assessment/Internal Assessment
Components (Drop
down)
Weightage (%)
Class Test
Assignment
Attendance
15
10
5
End Term
Examination
70
Lab/ Practical/ Studio Assessment:
Continuous Assessment/Internal Assessment
Components
(Drop down)
Class Test
(Practical
Based)
Practical
Records
Viva
End Term Examination
Practical
Practical
Performance Record
Viva
20%Weightage
Weightage (%)
Text:




15
5
10
40
10
20
Nelson DL & Cox MM,Lehninger Principles of Biochemistry 6th ed 2013, W.H. Freeman
Jocelyn E. Krebs & Elliott S. Goldstein, Genes XI, ISBN-13: 9781449659851
Harvey Lodish, Arnold Berk, Chris A. Kaiser and Monty Krieger, Molecular Cell Biology 7th ed 2012, ISBN-13: 978-1-4292-3413-9
Wilson K & Walker J,Principles and Techniques of Biochemistry and Molecular Biology7th ed 2010, Cambridge University Press
Remarks and Suggestions:
_______________________________
Date:
Name, Designation, Organisation