Download High hydrostatic pressure induces immunogenic cell

Published on Second Faculty of Medicine, Charles University (http://www.lf2.cuni.cz
)
High hydrostatic pressure induces immunogenic cell
death in human tumor cells.
PharmDr. Jitka Palich
Fučíková, Ph.D., Department
of Immunology
Abstract
Recent studies have identified
molecular events
characteristic of immunogenic
cell death (ICD), including
surface exposure of
calreticulin (CRT), the heat
shock proteins HSP70 and
HSP90, the release of highmobility group box protein 1
(HMGB1) and the release of
ATP from dying cells. We
investigated the potential of high hydrostatic pressure (HHP) to induce ICD in
human tumor cells. HHP induced the rapid expression of HSP70, HSP90 and CRT
on the cell surface. HHP also induced the release of HMGB1 and ATP. The
interaction of dendritic cells (DCs) with HHP-treated tumor cells led to a more
rapid rate of DC phagocytosis, upregulation of CD83, CD86 and HLA-DR and the
release of interleukin IL-6, IL-12p70 and TNF-α. DCs pulsed with tumor cells killed
by HHP induced high numbers of tumor-specific T cells. DCs pulsed with HHPtreated tumor cells also induced the lowest number of regulatory T cells. In
addition, we found that the key features of the endoplasmic reticulum stress-
mediated apoptotic pathway, such as reactive oxygen species production,
phosphorylation of the translation initiation factor eIF2α and activation of caspase8, were activated by HHP treatment. Therefore, HHP acts as a reliable and potent
inducer of ICD in human tumor cells.
http://onlinelibrary.wiley.com/doi/10.1002/ijc.28766/full
Published: 18. 11. 2015 / Responsible person: Mgr. Ing. Tereza Kůstková
Source URL (retrieved on 3. 8. 2017 - 13:21):
http://www.lf2.cuni.cz/en/faculty/major-publications/high-hydrostatic-pressureinduces-immunogenic-cell-death-in-human-tumor