Download Supplementary Table S4

Supplementary Table S4: Loss of oncogenic B-Raf signaling induces
transcripts associated with intestinal differentiation. Description of genes linked
to intestinal differentiation and listed in Figure 5B. Depending on the differential
regulation over time, genes might appear in only one cell line heat-map as only the
top 50 hits is shown for each cell line. However, if a significant change in expression
between DMSO and PLX4720 treated samples was detected, the gene is listed for
the other cell line as well (see Supplementary Table S2). The arrows mark the
mean direction of regulation over the time course. The abundance of transcripts at
the individual experimental time points is shown in Supplementary Fig. S8.
Gene
name
GSTA1
AKR1B10
HPGD
Cell line
Description
Ref.
Colo-205 ,
HT29 
Colo-205 
HT29 
Colo-205 ,
HT29 
Glutathione-S transferase; increased expression was reported for
differentiation Caco-2 cells
Aldo-keto-reductase; highly expressed in small intestine and colon under
physiological circumstances
Prostaglandine catabolism acting in antagonistic fashion to COX2; Negatively
regulated by -Catenin in colorectal cancer cell lines; Highly expressed in
differentiated murine and human intestinal cells; Loss-of-expression in human
colon cancer; Tumor suppressor in the APCMin mouse model; Deletion of the
murine Hpgd gene confers celecoxib resistance; Potential Cdx-2 target gene
Keratin; upregulated during vitamin D induced differentiation of KRAS-mutant
SW480 colon cancer cells
Mono-amin-oxidase, upregulated during vitamin D induced differentiation of
KRAS-mutant SW480 colon cancer cells
Highly expressed in terminally differentiated colorectal mucosa;
Downregulation of RARRES1 is related to stage D progression; Implicated as
tumor suppressor in various entities
Claudin-1; tight junction component; see main text for details
(1, 2)
KRT13
HT29 
MAOA
Colo-205 
RARRES1
Colo-205 ,
HT29 
CLDN1
Colo-205 ,
HT29 
Colo-205 
Colo-205 
Colo-205 
Colo-205 
CLDN15
CLDN18
CES1
NRARP
AMACR
Colo-205 ,
HT29 
UGT2B17
Colo-205 
MYO1A
Colo-205 ,
HT29 
VAV3
Colo-205 
PVRL3
HT29 
NDRG1
Colo-205 ,
HT29 
TFF3
Colo-205 ,
HT29 
Colo-205 ,
HT29 
PROM1
TXNIP
HMGCS2
Colo-205 ,
HT29 
Colo-205 ,
HT29 
Claudin-15; tight junction component; see main text for details
Claudin-18; tight junction component; see main text for details
Carboxylesterase 1; Potential Cdx-2 target gene
Notch1 target gene product with ankyrin repeats and negative feedback
regulator of this pathway; Expression of NRARP in murine intestinal tissues,
tumors and human CRC cell lines is positively correlated with expression of
Cdx-1 and Cdx-2
-Methylacyl-CoA racemase involved in β-oxidation of branched fatty and bile
acids; In normal colonic mucosa, expression is restricted to the surface
epithelium directly contacting the intestinal lumen; Expression is high in well to
moderately differentiated but not in anaplastic CRCs; Potential Cdx-2 target
gene; Loss of AMACR expression more frequently observed in proximal than
distal CRCs; Lack of staining or low-intensity staining correlated with poor
differentiation status, mucinous histology, lympho-vascular invasion and
microsatellite instability
UDP-glucuronosyltransferase, highly upregulated in differentiating Caco-2
cells
Myosin expressed in the apical brush border of enterocytes; Frequently
mutated or silenced in CRC; Acts as tumor suppressor upon ectopic
expression in human CRC lines
RhoGEFs regulating cytoskeletal dynamics; Vav3 is predominantly expressed
in apical regions of murine terminally differentiating cecal and colonic
enterocytes
Cell adhesion molecule of the nectin family localized to adherens junctions;
Increased expression was reported for differentiation in Caco-2 cells
Upregulated during colonic differentiation; ectopic expression in human CRC
lines induces hallmarks of differentiation and suppresses their invasive and
metastatic potential
Cdx-2 target gene and goblet cell differentiation marker; Component of
intestinal mucus.
Discussed as a marker of cancer stem cells in a variety of tumor entities; In
normal colon tissue, however, Prominin1 is primarily expressed on the
membrane of well-differentiated, Cdx-2-positive cells of the surface epithelium
Thioredoxin-interacting protein; Highly upregulated in differentiated colonic
epithelium
Expression of 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) increases
during differentiation of Caco-2 cells; Suppressed by Myc; Lost in poorly
differentiated CRC; Responsive to histone deacetylase inhibitors
(3)
(4-8)
(9)
(9)
(10, 11)
(2, 12)
(13)
(12, 14-16)
(17)
(18, 19)
(20)
(19)
(21, 22)
(23, 24)
(25)
(26)
(27)
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