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Download AH6 INVESTIGATOR Name Charles Walsh Address Department of
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AH6 INVESTIGATOR Name Charles Walsh Address Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260 IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity ANTIGEN Chemical properties Molecular weight nucleolus-like particles (NLP) Naegleria gruberi nucleoi mouse BALB/c ByJ female spleen 1990 IP Freund’s complete 1990 mouse P3-X63Ag8 IgG1, kappa light chain yes yes yes Naegleria gruberi; Saccharomyces cerevisiae, mouse (mammalian cells), and Caenorhabditis elegans nucleolar protein Naegleria gruberi, antigens are 125 kDa, 74 kDa, 60 kDa, 35 kDa, and 24 kDa. Saccharomyces cerevisiae, antigens are 96 kDa, 72 kDa and 58 kDa. Mouse (mammalian cells), antigen is 72 kDa. Caenorhabditis elegans, antigens are 66 kDa and 38 kDa. Characterization Immunoprecipitation yes Immunoblotting yes Purification yes Amino acid sequence analysis Functional effects Immunohistochemistry yes PUBLICATIONS : Trimbur, G.M., and Walsh, C.J. (1993). Nucleolus-like morphology produced during the in vitro reassociation of nucleolar components. J. Cell Biol. 122, 753-766. Trimbur, G.M., Goeckeler, J.L., Brodsky, J.L., and Walsh, C.J. (1999). Cloning, sequencing, and nucleolar targeting of the basalbody-binding nucleolar protein BN46/51. J. Cell Sci. 112, 1159-1168. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242
