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Name __________________________________________________________ Hour _______ Why Are Most Plants Green, But Some Are Not? Introduction A pigment is a molecule that absorbs light in the visible portion of the electromagnetic spectrum. The leaves of most plants are rich in pigments. These pigments absorb light and convert it into chemical energy to fuel the production of sugars. The primary photosynthetic pigment is chlorophyll a. Other pigments such as chlorophyll b and carotenoids are referred to as accessory pigments. These absorb light and funnel the energy to chlorophyll a. Different pigments absorb different types (wavelengths) of light. Some pigments might absorb blue light better than other wavelengths of light, for example. Others may absorb all the colors well, or none. A spectrophotometer is a machine used by scientists to measure the absorbance of light by substances. The better a pigment absorbs a color (wavelength) of light, the higher it’s percent of absorbance reading. The data in Table 6.1 give the spectrophotometer absorbance readings for chlorophyll a and chlorophyll b. Graphing Create a line graph using the graph paper provided by your teacher. Graph the data for chlorophyll a using a green colored pencil, the data for chlorophyll b using a blue colored pencil and the data for the carotenoids using a red colored pencil. Put all three lines on the same graph. Percent Absorption Readings For Chlorophyll a And Chlorophyll b Wavelength Chlorophyll a % Absorption Chlorophyll b % Absorption Carotenoid % Absorption 400 nm 32 8 425 nm 60 29 450 nm 10 62 475 nm 3 51 500 nm 0 8 525 nm 0 0 550 nm 4 3 575 nm 2 4 600 nm 4 2 625 nm 3 20 650 nm 21 29 675 nm 44 4 700 nm 12 0 Table 6.1: The percent absorption for plant pigments at various wavelengths. 22 23 49 43 55 34 0 0 0 0 0 0 0 Put the percent absorption on the Y-axis and the wavelengths on the X-axis. Make sure to give your graph and both of the axes’ titles. Don’t forget to include the necessary units. Use the long bar at the bottom of the graph to correctly color code the various wavelengths correctly. Use your textbook to discover which color corresponds to each wavelength. Now use your graph to answer the following questions. Analysis 1. Based on the data and your graph, why is chlorophyll green? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 2. Why are most plants green? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 3. How can some plants perform photosynthesis if they are not green? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 4. In what way are chlorophyll a and chlorophyll b similar in their ability to absorb wavelengths? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 5. In what way are chlorophyll a and chlorophyll b different in their ability to absorb wavelengths? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 6. Which wavelengths can the carotenoids absorb well that both of the chlorophylls cannot? ______________________________________________________________________________ 7. Which types of light are the most important to plants for photosynthesis? ______________________________________________________________________________ ______________________________________________________________________________ 8. Design an experiment that would prove your hypothesis from question #7. ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 9. What colors are carotenoids? ______________________________________________________________________________ ______________________________________________________________________________ 10. What do carotenoids do for plants? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 11. Here in Michigan, the leaves of trees change color in the fall. Explain why and relate your explanation to carotenoids. ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________