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KAPA PURE BEADS Attract what matters. Benefits include: KAPA Pure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA and RNA next-generation sequencing library construction workflows. • high recovery of single- and double-stranded DNA (1 ng – 5 µg) in a single cleanup • fast and efficient cleanups to remove unwanted reaction components • easy substitution into bead-based workflows • enables adjustable size selection • automation friendly Seamless Integration into NGS Workflows • Compatible with all KAPA DNA and RNA library preparation protocols • Achieve equivalent yields and size distribution in comparison to Agencourt® AMPure® XP • Readily incorporated into existing automation applications A50 50 B 40 40 Post-ligation Yield (nM) Post-ligation Yield (nM) Post-ligation Yield (nM) Post-ligation Yield (nM) 60 60 20 20 40 40 10 10 20 20 0 0 0 0 KAPA KAPA Pure Pure Beads Beads 160160 140140 KAPA Pure Beads provides equivalent performance to Agencourt® AMPure® XP in both DNA and RNA workflows. 80 80 30 30 180180 100100 KAPA KAPA PurePure KAPA KAPA PurePure KAPA KAPA PurePure AMPure AMPure XP XP KAPA KAPA Pure Pure Beads Beads 180180 AMPure AMPure XP XP AMPure AMPure XP XP AMPure AMPure XP XP 160160 140140 120120 AMPure AMPure XP XP KAPA KAPA PurePure KAPA KAPA PurePure AMPure AMPure XP XP KAPA KAPA PurePure AMPure AMPure XP XP AMPure AMPure XP XP Fluorescence Fluorescence Fluorescence Fluorescence 120120 100100 100100 80 80 80 80 60 60 60 60 40 40 40 40 20 20 20 20 0 0 0 0 -20-20 -20-20 35 35100100200200300300 400400 600600 1000 1000 10380 10380 bp bp Unless otherwise stated in references, presented data on file For Research Use Only. Not for use in diagnostic procedures. 35 35100100200200300300 400400 600600 1000 1000 bp bp 10380 10380 A) Libraries were prepared with the KAPA HyperPlus Kit, from 100 ng high-quality E. coli genomic DNA, fragmented at 37˚C for 30 min. to target a final library size of 300 bp. B) Libraries were prepared with the KAPA Stranded RNA-Seq Kit with RiboErase, from 100 ng of Universal Human Reference (UHR) RNA according to standard protocol. Yields were measured with the KAPA Library Quantification Kit post ligation. Electropherograms for all libraries were generated with a Bioanalyzer 2100 High Sensitivity DNA Kit. Tunable and Highly Reproducible Size Selection • Obtain consistent library size distributions • Flexible implementation at various points during library construction • Adjustable size selection parameters to achieve desired library sizes A B Size selected after amplification (0.6X – 0.8X) Amplified Library (no size selection) 600 Size selected after amplification (0.6X – 0.8X) 800 400 Amplified Library (no size selection) 600 200 400 0 10 50 100 150180 300 500 1000 2000 Size selected after amplification (0.6X – 0.8X) 800 Fluorescence Fluorescence Fluorescence Fluorescence 800 10000 Amplified Library (no size selection) 600 Size selected after amplification (0.6X – 0.8X) 800 400 Amplified Library (no size selection) 600 200 400 0 10 50 100 150180 300 500 1000 2000 10000 200 bp bp Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA Hyper Prep Kit, from 1 µg high-quality E. coli genomic DNA, fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 0 2000 160 10 50 (0.6X 100 150 300 500 KAPA 1000 2000 10000 (A) or Agencourt® AMPure® XP 10(B) 50was 100performed 150180 300 500 1000 2000 10000 350 – 450 bp. Size selection –180 0.8X) using either Pure Beads after library amplification bp 140 Sensitivity Assay. (2 cycles). Electropherograms were generatedbp with a PerkinElmer® LabChip GX DNA High 200 200 Fragmented DNA (no size selection) Size selected after fragmentation (0.6X–0.8X) Fragmented DNA (no size selection) Size selected ligation Size after selected after (0.5X–0.8X) fragmentation (0.6X–0.8X) 100 150 D Fluorescence Fluorescence C Fluorescence Fluorescence 150 50 Size selected after ligation (0.5X–0.8X) 100 0 50 120 160 100 140 80 Amplified library (no size selection) 120 60 0.5X – 0.7X 100 40 0.6X – 0.8X Amplified library 0.7X – 0.9X (no size selection) 80 20 0.5X – 0.7X 60 0 0.6X – 0.8X 40 35 100 150 200 300 400 500 600 1000 2000 35 10380 0 300 0.7X – 0.9X 20 bp 100 150 200 400 500 600 1000 2000 10380 500 600 1000 2000 10380 bp 0 35 100 150 200 300 400 500 600 1000 2000 10380 35 100 150 200 300 400 bp bp Adjustable size selection at various points in library preparation. (C) Equivalent final library size is achieved by performing size selection either immediately after genomic DNA fragmentation or after adapter-ligation. (D) Various final library sizes are achieved by timing size selection parameters after adapter-ligation. All libraries were prepared with the KAPA Hyper Prep Kit, from 100 ng high-quality human genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Electropherograms were generated with a Bioanalyzer 2100 High Sensitivity DNA Kit. Ordering Information Roche Cat. No. Kapa Code Description Kit Size 07983271001 KK8000 KAPA Pure Beads 5 mL 07983280001 KK8001 KAPA Pure Beads 30 mL 07983298001 KK8002 KAPA Pure Beads 60 mL @KapaBiosystems Headquarters, United States Wilmington, Massachusetts Tel: 781.497.2933 Fax: 781.497.2934 [email protected] KapaBiosystems KapaBiosystems International Office Cape Town, South Africa Tel: +27.21.448.8200 Fax: +27.21.448.6503 [email protected] Kapa Technical Support kapabiosystems.com/support Unless otherwise stated in references, presented data on file © 2016 Kapa Biosystems. All trademarks are the property of their respective owners. For Research Use Only. Not for use in diagnostic procedures. SS113001 A172 5/16