Download 11 June 2015, Amsterdam

Poster Presentation
Abstract Submission Form
11 June 2015, Amsterdam
During the conference (young) scientists will have the opportunity
to present non-commercial posters.
Abstracts can be submitted using this form only.
We have limited space available. Abstracts received at [email protected] before 6 April 2015 will be
considered for poster presentation. Selection will be done by the Joaquin partnership and
conference organization. E-mail notification of acceptance or rejection will be sent to the
submitting author no later than 1 May 2015. Further details and requirements will be given upon
acceptance.
Poster Title
Determination of Estrogenic Activity in PM10 Air Samples
from Flanders (Belgium) using the BG1 Ovarian Cancer Cell
Line
Authors and Affiliations
K. CROES1, R. VAN DEN HEUVEL2, M. PLUSQUIN3, J.
STAELENS4, B. VAN DEN BRIL4, M.S. DENISON5, T.
VANDERMARKEN1, K. VAN LANGENHOVEN1, M.
ELSKENS1
1
Department of Analytical, Environmental and Geo-Chemistry
(AMGC), Vrije Universiteit Brussel; Pleinlaan 2, 1050 Brussels,
Belgium; [email protected], [email protected],
[email protected], [email protected]
2
Environmental Health and Risk, VITO; Boeretang 200, 2400 Mol,
Belgium; [email protected],
3
Interuniversity Institute for Biostatistics and Statistical
Bioinformatics, Hasselt University; Agoralaan 1, 3590
Diepenbeek, Belgium; [email protected]
4
Unit Air, Flemish Environment Agency (VMM);
Kronenburgstraat 45, 2000 Antwerp, Belgium;
[email protected], [email protected]
5
Department of Environmental Toxicology, University of
California; Davis, CA 95616 USA; [email protected]
Abstract
(max 250 words)
The abstract should state briefly
and clearly the Objective, Methods,
Results and Conclusions of the
work. Do not include tables or
figures.
Endocrine disrupting chemicals (EDCs) represent a broad class of
compounds, are widespread in the environment and can pose
severe health effects. In this study, a novel methodology to
measure the overall response of EDCs in PM10 air samples was
developed and the effect of different extraction solvents was
investigated. Therefore, a bioassay technique using a BG1Luc4E2
ovarian carcinoma cell line was employed to quantify the potency
of the mixture of estrogenic compounds in PM10 air samples.
Between April 2013 and January 2014, samples were taken in a
rural, industrial and urban area in Flanders (Belgium). Estrogen
disruptive potencies were reported as biologic equivalents (BEQ),
expressed in pg 17β-estradiol/m³ air. Estrogenic activity was
measured in more than 70% of the samples. No statistical
significant differences in estrogenic activity were found between
the locations, but large day-by-day variations were seen in all
locations. The estrogenic activity was positively related with PM10
levels, black carbon, pyrene and fluoranthene, while a significant
negative correlation with air temperature was found. Furthermore,
the estrogenic potency was generally higher when a polar
extraction solvent was used (ethanol or acetonitrile) instead of a
hexane/acetone (1/1) mixture. This study shows that the in vitro
bioassay is a suitable tool to monitor estrogenic disruption in air
samples. However, it seems that changes in sample pre-treatment
methods can significantly influence the observed potencies and
that a harmonized approach for sampling, extraction and in vitro
measurement is thus necessary.
Contact Name and Position
Organisation
Vrije Universiteit Brussel (VUB)
Address
Pleinlaan 2
City
Brussels
Postal Code
1050
Country
Belgium
Telephone
+32 2 629 32 93
E-mail
[email protected]