Download enzymatic hydrolysis of proteins into tailored peptides and

ENZYMATIC HYDROLYSIS OF PROTEINS INTO TAILORED PEPTIDES AND THEIR
USE IN DIFFERENT APPLICATIONS
Roel Vleeschouwers, W . Dejonghe, K.Elst
Flemish Institute for Technological Research
[email protected]
In recent years, it has been reported that various peptides isolated from chemical or enzymatic
hydrolysis of many food proteins or microbial fermentation exert a number of activities affecting the
gastrointestinal, endocrine, cardiovascular, immune and nervous systems. Lately these peptide
hydrolysates are also used as functional food ingredients in infants, elderly as well as sport nutrition.
Also applications in coatings and adhesive products are well known. The traditional hydrochloric acid
hydrolysis may form toxicologically critical substances, such as 3-chloro-1,2-propanediol (MCPD)
and 1,3-dichloro-2-propanol (DCP), and results in high sodium chloride concentrations in the
neutralized products. The enzymatic processing alternative, introduced into industry recently,
provides more sustainable and environmentally friendly conditions. Batch-type hydrolysis is the
established route to produce protein hydrolysates in food and pharmaceutical industries. However,
the hydrolytic efficiency of peptidases decreases due to accumulation of the soluble peptides and
free L-amino acids. This is a general phenomenon since it has been observed with various
peptidases and different substrates e.g. soy protein, casein, gelatin, and wheat and corn gluten.
Thus, high peptidase activities and long incubation times are required, reducing the economic
viability of this procedure. Several studies indicated that the enzyme membrane reactor allowed
rapid separation of low-molecular-weight peptides from protein hydrolysates, thus avoiding the
disadvantages of batch reaction such as inefficient use of enzymes, inconsistent products due to
batch-to-batch variation, substrate–product inhibition, low productivity and excessive hydrolysis
leading to bitter peptides and amino acids instead of peptides. In addition, immobilization of these
peptidases could prevent autolysis and preserving their activity after several runs.
This project is intended to develop and optimize an enzymatic hydrolysis process that is combined
with an off line (fractionation) or an on line (enzyme membrane reactor) filtration step to obtain
tailored peptides from different protein sources. On the one hand we will optimize these two
enzymatic hydrolysis processes to increase peptide yield, volumetric productivity as well as the long
term use of the enzyme. On the other hand, we will tailor the peptide length and test the functionality
of these tailored peptides in different applications such as food (infants, elderly, sport nutrition), feed,
nutraceuticals, coatings, adhesives, antimicrobial agents, polymer chemistry (n a t u r a l
p o l ya m i d e s ) , p a c k a g i n g m a t e r i a l ….
o
t h e f i e l d s o f a p p l i c a t i o n s o r t yp e s o f p a r t n e r s yo u a r e l o o k i n g f o r
Partners active in production of peptides and use of these peptides in different applications in food
(infants, elderly, sport nutrition), feed, nutraceuticals, coatings, adhesives, antimicrobial agents,
polymer chemistry (n a t u r a l p o l ya m i d e s ) , p a c k a g i n g m a t e r i a l …..
o
Poster + flyer
t h e w a y i n w h i c h yo u w a n t t o p r e s e n t yo u r p i t c h ( p o w e r p o i n t , p o s t e r
or other)