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Smooth vs wrinkled peas R – Round r – wrinkled During Seed development R_ x rr Enzyme converts Sugar to starch. Defective enzyme inefficient conversion of sugar to starch. No sugar so no excess H2O taken up as seed develops. Sugar accumulates, Water taken up as seed develops. When seed dries out, smooth. When seed dries out, wrinkled. Starch is made up of amylopectin and amylose. Starch is how plants store glucose, (we store it as glycogen). Both are polymers of glucose. Amylopectin: - Insoluble in water. - Highly branched, 2000 to 200,000 glucose units. - Glucose units linked in linear way with branching every 24-30 glucose units. Amylose: - Insoluble in water. - Helix structure, 300 to 3000 glucose units. - Hard to digest but takes up less space so preferred. - Iodine molecules fit inside helical structure and bind, causing it to absorb certain wavelengths of light to show as a blue-black color. Starch doesn't absorb water osmotically as much as sugar does. The real difference is the concentration of osmotically active particles. The difference between the peas is in a gene for an enzyme that polymerizes monosaccharides into polysaccharides. In the smooth peas, the enzyme is expressed normally, and the enzyme makes the sugar into starch at a rapid rate. As a result, most of the sugar in the pea is converted into starch early on. This results in large starch granules. That means that there are fewer osmotically active particles, and the seed (the pea) absorbs less water osmotically from the parent plant while it is developing in the pod. Having absorbed less water in development, the seed has less to lose when dried and its seed coat is smooth in the dried state because less volume is lost. In the wrinkled pea, there is a recessive mutation in the same gene where the enzyme that polymerizes sugar into starch is defective. As a result, there is an accumulation of sugar in the seed (therefore smaller starch granules), and more water is accumulated osmotically by the sugar. The seed, while in the pod, absorbs more water and swells to a greater size. When dried, there is more water volume to be lost. The seed coat, having been stretched to a greater size initially, falls into wrinkles when the seed dries.