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Project information of Ottawa-SJTU Summer Program 2017 1、Title: Isolation of organelles from yeast cells Introduction:(in 200 words) The emergence of eukaryotic cells is a major milestone in the evolution of life. All current multicellular organisms are eukaryotic. Eukaryotic cells are more sophisticated than prokaryotic ones in many aspects, one of which is subcellular compartmentalization based on the endomembrane system. These compartments are conceptually assigned to organelles. The aim of this training project is to obtain a first-hand experience in the observation and isolation of organelles, using the budding yeast as a model. Students will learn to work with yeast liquid culture, plasmid transformation, fluorescent microscopy, protein immune-blotting, density gradient centrifugation, and other related techniques. Lab Leader XIE, Zhiping [email protected], Mobile 18516609713 Tutor XIE, Zhiping [email protected], Mobile 18516609713 Partner Graduate Students CUI, Xuefei [email protected], Mobile 17740815954 The demands General background in biology or biomedical sciences. Prior experiences in yeast biology, protein electrophoresis, optical microscopy encouraged.