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Transcript
Project information of Ottawa-SJTU Summer Program 2017
1、Title:
Isolation of organelles from yeast cells
Introduction:(in 200 words)
The emergence of eukaryotic cells is a major milestone in the evolution of life. All current
multicellular organisms are eukaryotic. Eukaryotic cells are more sophisticated than prokaryotic
ones in many aspects, one of which is subcellular compartmentalization based on the
endomembrane system. These compartments are conceptually assigned to organelles. The aim of
this training project is to obtain a first-hand experience in the observation and isolation of
organelles, using the budding yeast as a model. Students will learn to work with yeast liquid culture,
plasmid transformation, fluorescent microscopy, protein immune-blotting, density gradient
centrifugation, and other related techniques.
Lab Leader XIE, Zhiping [email protected], Mobile 18516609713
Tutor
XIE, Zhiping [email protected], Mobile 18516609713
Partner Graduate Students CUI, Xuefei [email protected], Mobile 17740815954
The demands General background in biology or biomedical sciences. Prior experiences in yeast
biology, protein electrophoresis, optical microscopy encouraged.