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Download Laboratory Three: Cellular Morphology and the Microscope
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Parts of the Brightfield Microscope and their Function Part Function Ocular lens Magnifies the image 10X. Nosepiece Contains the objective lenses. Objective lenses Magnifies the image 4, 10, 40 or 100X. Stage Holds the slide and usually has clips to hold slide stationary. Diaphragm Adjusts the amount of light to the lens. Condenser Focuses light into the specimen. Light Source Produces and adjusts the intensity of the light. Rheostat Adjusts the intensity of the light. Course focus Focuses image when using the 4 and 10X lenses. Fine Focus Focuses the image when using the 40 and 100X lenses Mechanical Stage Adjuster Moves the slide on the stage Lab 2 Instructions Observing specimens with brightfield microscope. 1. Use pages 75-83 to view the slides indicated by your instructor (shown below). Use your brightfield scope to view these specimens. Know the following slides at the indicated magnifications. Remember: You must first focus each slide under 100X first before viewing under 400X and you must view under 40X before viewing under 1000X. Slides Taxonomic Group Anabaena Prokaryote Cyanobacteria Eukaryote Algae Eukaryote Amoeba Diatoms Mixed amoebic infection smear and any other amoeba slides we have Schistosoma mansonni (eggs) Schistosoma mansonni cercaria Peridinium Trypanosoma gambiensae Mold types Eukaryote Animalia Eukaryote Animalia Eukaryote Algae Eukaryote Protist Eukaryote Fungus Mold Final Magnification to know. 400X 400X 1000X 400X 100X 400X 1000X 400X -1- Yeast or saccharomyces Bacterial coccus Bacterial Bacillus Bacterial spirillum Eukaryote Fungus Yeast Prokaryote Bacteria Prokaryote Bacteria Prokaryote Bacteria 1000X 1000X 1000X 1000X -2-
