Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
What is so Interesting About Fetal Healing? Spina Bifida 100% chance of being Paralyzed Fetal Surgery? NIH Study Hundreds of Surgeries Performed 50% chance of success now Unexpected Find? Despite Large Incisions during Surgery No Scars… (https://www.youtube.com/watch?v=iP9LlyHDXpc) Amniotic Membrane Regulates Healing Response to Trauma Repair Regeneration Protect: Scar Restore to Original (1992 - Scarless Fetal Healing, Therapeutic Implications (Adzick)) Amniotic Membrane Regulates Healing Growth Factors Cytokines Promotes Cell Proliferation Regulate Cell Proliferation (2012 - AM, From Structure and Functions to Clinical Applications (Mamede)) History of Amniotic Membrane (AM) What is Amniotic Membrane? • Placental based Tissue harvested from Living Donors • First used in 1910 as wound dressing • 1990’s ocular • Today: Surgical Barrier, Wound Care, Dressing, Ocular, Spine, F&A…. Amniotic Membrane How can amniotic membrane help prevent adhesions? 1. Physical Separation 2. Chemical Signaling Ideal Wound Barrier or Soft Tissue Wrap 1. Physical Separation Ideal Wound Barrier 2. Chemical Signaling 1. Physical Separation: Stays in the Body Past Critical Healing Stage Stages of Healing 1. Injury/Bleeding – – 2. Inflammation – – – 3. “Cleans up the debris” Protects against infection Becomes the bad guy eventually (Remember this!) Proliferation – – – – 4. “Caps the Pipes” Body sends coagulants “Build a new Framework” Cell rebuilding and growth Fibrosis can get out of control Scarring Remodeling (1989 - Phys Therapy - The Biology of Scar Formation (Hardy 1989)) 1. Physical Separation: Stays in the Body Past Critical Healing Stage Inflam Proliferation Tissue Presence High Remodeling AlloWrap Other Barriers Low 0 10 20 30 Days in Healing Process 40 1. Physical Separation: Stays in the Body Past Critical Healing Stage HUMAN AMNIOTIC TISSUE AS AN ANTI-ADHESION, ANTI-FIBROTIC BARRIER IN AN OVINE SPINAL LAMINECTOMY MODEL • Collaboration with Colorado State University • Ovine Laminectomy Model • Study Design – – 8 weeks in-vivo – Two implants per sheep – Locations: (L5/L4; L2/L3) Conclusion: At 8 weeks, AlloWrap was still present as a surgical barrier. 1. Physical Separation: Stays in the Body Past Critical Healing Stage ALLOWRAP® SURGICAL BARRIER REMAINS IN THE BODY THROUGH THE CELL PROLIFERATION PHASE OF THE HEALING PROCESS WHILE OTHER SURGICAL BARRIERS ARE RESORBED • Evaluation of the critical stages of healing • Comparison of other surgical barriers • Conclusion: AlloWrap remains in the body passed the cell proliferation stage of healing and into the remodeling stage Ideal Wound Barrier or Soft Tissue Wrap 1. Physical Separation Ideal Wound Barrier 2. Chemical Signaling 2. Chemical Signaling – Growth Factor Regulation Growth Factors Cytokines Promotes Cell Proliferation Regulate Cell Proliferation 2. Chemical Signaling – Anti-Inflammatory Inflammation Proliferation Growth Factors Cytokines Promotes Cell Proliferation Regulate Cell Proliferation IL-10 HLA IL-8 • • • IL-8-pro-inflammatory chemokine HLA-ligand to CD44 which is expressed on inflammatory cell Stromal matrix signals (2004 - Laboratory Science - How Does Amniotic Membrane Work (Tseng)) Stromal 2. Chemical Signaling – Anti-Scarring Inflammation IL-8 FGF IL-6 Proliferation Growth Factors Cytokines Promotes Cell Proliferation Regulate Cell Proliferation IL-10 TGF-β TNF- α • • • • FGF Amniotic Membrane down regulates TGF-B Fibroblast response to scar-promoting cytokines is different in adults vs. infants IL-10 suppresses IL-6, IL-8, and TNF-α Production of Neurotrophic factors-promote scarless healing (2004 - Laboratory Science - How Does Amniotic Membrane Work (Tseng)) 2. Chemical Signaling – Anti-Microbial Defensin • Defensins – – • Epithelial cell surfaces are antimicrobial Bind to bugs and destroy them Elastase Inhibitors – – Secrete proteinase inhibitor Elafin Inactivates invading microbes 2001 - Journal of Bio Chem - Isolation & Char of Human b-Defensin-3, a Novel Human Inducible Peptide Antibiotic (Harder) 2003 - Reprod Biology & Endo - Innate Immune Defences in the Human Endometrium (King) Science of Amniotic Membrane: Immune Privilege Body Response to Foreign Object • Surgical Intervention 2 • Foreign Object Detected 1 (1995 - Unraveling Immune Privilege (Streilein)) • Immune response by Body 3 4 • Antibodies Invade & Fight • Delayed Healing 5 Science of Amniotic Membrane: Immune Privilege Why Doesn’t the Body See AM as a Foreign Body? • If the body sees foreign DNA, the body will attack cells • Every fetus contains foreign DNA • AM protects the fetus from being identified as a foreign entity. • Therefore, AM is naturally Immune Privilege. (1995 - Unraveling Immune Privilege (Streilein)) Amniotic Membrane How can amniotic membrane help prevent adhesions? 1. Physical Separation • Remains in the body past fibrosis stage • Other therapies dissolve too quickly 2. Chemical Signaling • Down Regulates Inflammation • Down Regulates Fibrosis which causes scarring How has Amniotic Membrane Been Used? Burn (Ocular or Facial) Craniotomy (Tomita) (Branski, Safawi) Laminectomy (Sameniego, Tao, Choi) Ulnar or Flexor Nerves (Kim, Demirkan, Ozgenel) Tethered Cord (Nakaji) Abdominal Repair or OBGYN Sciatic Nerve (diZerega) Achilles (Jay) Diabetic Wounds Achilles, Tarsal Tunnel, or Neuroma (Jay, Ng)