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Microbiology (Bio 370)
Practice Lecture Examination 8
2/5/2014 Kimberly
Name _____KEY________
I. Define: (do not use examples as definitions)
1. septicemia - an actively dividing pathogen within the blood
2. psychrophil - a bacterium that grows/lives in cold environments (0 to -20C)
3. loop dilution - a method of diluting bacteria from a concentrated source.
One loop full into a sterile broth, agitate and then take a sterile loop and transfer mixture.
4. bacteremia - bacteria in the blood without determining if they are growing or dead
5. cystitis - infection of the urinary bladder
II. Multiple Choice - Choose the most appropriate answer!
1. Kimberly suggested UTIs would be more problematic in this group: a) athletic males;
b) women; c) males under the influence of puberty; d) older males; e) both a & c; f) b & d
2. Where would I most likely see halophils growing? a) foot pad of an armadillo;
b) smegma of the groin; c) drinking fountains; d) estuaries; e) nursing homes
3. Kimberly suggested spore-formers are primarily in this group: a) Gram positives; b) Gram neg.;
c) acid fast bugs; d) strict anaerobes; e) strict aerobes
4. Kimberly suggested three streaking methods on a plate for isolating colonies. Which one was not
listed? a) loop transfer; b) continuous; c) radial; d) quadrant
5. Kimberly suggested one of these is difficult to complete Koch’s postulates because of incubation
problems. Which one? a) TB; b) leprosy; c) boils; d) strept throat; e) bacterial meningitis
III. True or False - Correct the false
___F__ 1. (Once again) As magnification goes up, resolution goes up. (resolution goes down)
___F__ 2. The acronym, HTST, stands for ‘high tolerance spore threshold.’ (high temp short time)
___F__ 3. Gram positives have the most ‘complex’ cell envelope. (Gram negatives)
___F__ 4. Using the spore stain we discussed (Schaeffer Fulton), the spore would be colored red.
(green)
___F__ 5. Kimberly suggests the best way to avoid pathogens is to drink bottled water. (washing
hands)
IV. Fill in the blanks.
1. A method of concentrating bacteria from a liquid sample would be: __membrane filtering___.
2. Which is irreversible for a spore - germination or sporulation? ___germination___.
3. Getting a sterile sample of urine is difficult. Why? _normal urethral flora mixed with sample
_____epipubic sampling into urinary bladder - too painful and invasive______________
4. Kimberly suggested thermophils grow above this temperature: ___>50C___.
5. 98.6 degrees Fahrenheit equals ___37.5 C__ centigrade.
V. Essay - How would I get a 1:1000 diluted sample of milk?