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EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles EasySep™ Release Kits You can now positively select immune cells and then release bound magnetic particles from your isolated cells. EasySep™ Release combines the speed and ease of the EasySep™ cell isolation platform with the flexibility of the new Releasable RapidSpheres™ for the separation of particle-free, highly purified immune cells. Mix and match with EasySep™ cell isolation kits that use Dextran RapidSpheres™ to isolate unique and complex cell types by performing sequential positive selections. Learn more and see data at www.easyseprelease.com. POSITIVE SELECTION CATALOG # Human CD3 17751 Human CD4 17752 Human CD19 17754 Human PE 17654 Human Biotin 17653 Mouse PE 17656 Mouse Biotin 17655 Why Use EasySep™ Release? PARTICLE-FREE. Culture and analyze positively selected cells without magnetic particles. Why Choose EasySep™? FLEXIBLE. Perform sequential positive selections to isolate unique and complex cell types. “The viability of the cells tends to be higher just because it is so fast.” - Scientist, National Institutes of Health FAST AND EASY. Obtain highly purified cells free of magnetic particles in under 30 minutes. “More cost-effective due to the lack of consumables.” - Postdoctoral Fellow, Yale School of Medicine COLUMN-FREE. With no columns, the simple EasySep™ pour-off is gentle on your cells. “Very flexible and allows for easy up-scaling.” - PhD Candidate, University of Oxford HIGH PURITY. Achieve purities of up to 99%. Which Cell Isolation Platform Should You Choose? EasySep™ For fast and easy cell separation with a simple pour EasyEights™ & EasyPlate™ For simultaneous cell separation from up to 96 samples | Scientists Helping Scientists™ TOLL FREE PHONE 1 800 667 0322 • WWW.STEMCELL.COM PHONE +1 604 877 0713 FOR GLOBAL CONTACT DETAILS VISIT OUR WEBSITE • DOCUMENT #DX27021 [email protected] • VERSION 1.3.0 JAN 2017 [email protected] EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles How Does EasySep™ Release Work? Typical EasySep™ Release Protocol 1 2 Add EasySep™ Release Positive Selection Cocktail. 3 Add EasySep™ Releasable RapidSpheres™. 5 4 Place tube in EasySep™ Magnet and incubate for 5 minutes. Resuspend cells and add EasySep™ Release Buffer. Place tube in EasySep™ Magnet and incubate for 5 minutes. Pour off to discard supernatant. 6 Resuspend cells and repeat for a total of 3 x 5-minute separations. Incubate 3 minutes Pour off desired fraction into a new tube. Incubate 3 minutes Incubate 3 minutes Cell suspension Highly Purified Cells Start Isolated Start Typical EasySep™ Release Human CD3 Positive Selection + Starting with a single-cell suspension of human PBMCs, the CD3 cell content of the isolated fraction is typically 98.7 ± 0.9% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 38.4% and 99.0%, respectively. Isolated Typical EasySep™ Release Human CD19 Positive Selection Starting with a single-cell suspension of human PBMCs, the CD19+ cell content of the isolated fraction is typically 97.7 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.2% and 98.0%, respectively. For a complete list of EasySep™ cell isolation kits, please visit www.EasySep.com. Copyright © 2017 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, Shield Design, Scientists Helping Scientists, EasySep, EasyEights, EasyPlate, and RapidSpheres are trademarks of STEMCELL Technologies Canada Inc. All other trademarks are the property of their respective holders. STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.