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Topic 08 Mitosis I. Mitotic Cell Division A. Mitosis – used for growth, repair, and asexual reproduction - the chromosomes in the nucleus are duplicated - the cell divides along with the cytoplasm to form two identical cells - occurs in stages – the cells produced are identical to each other and the original cell they came from – 1. Interphase - time between cell divisions - the chromosomes are replicated (duplicated) - the two identical strands are attached at the centromere and the individual strands are known as chromatids a. A closer look at interphase – most of the time is spent in interphase (1)3 stages – G1, S, and G2 – (a.)G1 – intense biochemical and biosynthetic activity and growth – the cell doubles in size and new organelles such as mitochondria, ribosomes, and centrioles are produced. – (b.) S – synthesis of DNA takes place – each chromosome is replicated – (c.) G2 – cell prepares for mitosis by making any of the components that the cell needs to complete cell division – 2. Prophase - spindle fibers become visible - nuclear membrane disintegrates – first actual step of mitosis – the chromatin shortens and thickens to become chromosomes – 3. Metaphase - the double stranded chromosomes become attached at the spindle fibers and move to the center of the cell (equator) – second step of mitosis 4. Anaphase - centromeres replicate and then separate - spindle fibers shorten and pull the chromatids apart – 3rd step – 5. Telophase - nuclear membrane forms around each new set of chromosomes - the new nuclei have the same number and types of chromosomes as the original nucleus – last step B. cytoplasmic division – also known as cytokinesis - at the end of mitosis, the cytoplasm divides and you have two new daughter cells - if this does not occur, you end up with one cell that has two nuclei Stages of mitosis Stages of Mitosis C. differences between mitotic cell division in plant and animal cells – – 1. Animal cells contain centrioles which aid in cell division 2. In plant cells, a cell plate is formed that becomes the new cell wall. In animal cells, the cell pinches in to form two separate cells Mitosis Animation http://vcell.ndsu.nodak.edu/animat ions/ D. uncontrolled cell division - invades surrounding tissue and interferes with their normal functions - known as cancer II. Asexual Reproduction A. definition - does not include two parents or the fusion of two nuclei - has one parent that reproduces by mitosis B. methods of asexual reproduction – 1. Binary fission - cytoplasm and nuclear material divide equally to form two daughter cells that are equal in size – 2. Budding - a new organism grows off the side of the parent - may either separate or live as a colony - the daughter cell is smaller than the parent cell – 3. Sporulation - spores are released by the parent - when conditions in the environment are favorable, they will produce new organisms – 4. Regeneration - when lost parts grow into new organisms - ex. Starfish and planaria – 5. Vegetative propagation a. bulbs - enlarged, underground stems - surrounded by leaves - grows into a new plant – example: various flowers b. tubers - enlarged, underground stem with buds grows into a new plant – example: potato c. runners - horizontal stems that grow along the ground - these stems enter the ground and grow into new plants – example: strawberries d. cuttings - a piece of a plant is put in water and grows roots which can be planted e. grafting - put two plants together III. Biotechnology A. Cloning - producing identical offspring from a single cell of an organism – take DNA from one organism and transfer it to an zygote – see board Click and Clone B. Genetic engineering - transfer of genes from one organism to another producing recombinant DNA – see example on board C. Human Genome – mapped out human DNA