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Glucocorticoids transform CD40-triggering of dendritic cells into an alternative activation pathway resulting in antigen-presenting cells that secrete IL-10 by Delphine Rea, Cees van Kooten, Krista E. van Meijgaarden, Tom H. M. Ottenhoff, Cornelis J. M. Melief, and Rienk Offringa Blood Volume 95(10):3162-3167 May 15, 2000 ©2000 by American Society of Hematology Pretreatment with DEX inhibits the phenotypic changes induced by CD40 ligation.Seven-day immature DCs were cultured for 24 hours in the absence or the presence of 10−6 mol/L DEX and activated via CD40 with the CD8-CD40L fusion protein for 48 hours. Delphine Rea et al. Blood 2000;95:3162-3167 ©2000 by American Society of Hematology DCs triggered through CD40 maintain an activated phenotype on a subsequent DEX exposure.Immature DCs were activated with the CD8-CD40L fusion protein. Delphine Rea et al. Blood 2000;95:3162-3167 ©2000 by American Society of Hematology Pretreatment with DEX does not affect the regulation of DC-antigen uptake machinery.Immature DCs were incubated in the absence or the presence of 10−6 mol/L DEX for 24 hours and further activated or not via CD40 with the CD8-CD40L fusion protein for 48 hours. Delphine Rea et al. Blood 2000;95:3162-3167 ©2000 by American Society of Hematology Pretreatment with DEX alters the cytokine secretion profile of CD40-triggered DCs.DEX-exposed or control immature DCs were left in culture without further treatment or stimulated with the CD8-CD40L fusion protein. Delphine Rea et al. Blood 2000;95:3162-3167 ©2000 by American Society of Hematology Pretreatment with DEX impairs the T-cell stimulatory capacities of DCs activated via CD40.Allogeneic mixed lymphocyte reaction: nonadherent allogeneic peripheral blood mononuclear cells were cultured with different numbers of CD40-triggered DCs, DEX-treated... Delphine Rea et al. Blood 2000;95:3162-3167 ©2000 by American Society of Hematology DEX-treated DCs triggered through CD40 induce a state of hyporesponsiveness in Th1 cells.Hsp65-specific T cells precultured with CD40-triggered DCs or with immature DCs or with DEX-treated CD40-triggered DCs pulsed with the p3-13 peptide epitope were harves... Delphine Rea et al. Blood 2000;95:3162-3167 ©2000 by American Society of Hematology
