Download Course Outline - Pima Community College

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PIMA COMMUNITY COLLEGE
Effective Term:
BIO
132
Credit Hours:
Fall 2016
Biosciences II: Laboratory Research
3.00
Lecture Periods:
2.00
Lab Periods:
3.00
Description:
Continuation of Biosciences I, with an emphasis in human genetics and biotechnology. Includes career
exploration, history and application of recombinant DNA technology, fundamentals of cell biology and genetics,
the Human Genome Project, and bioethics. Also includes an emphasis on a variety of advanced biotechniques
and skills.
Prerequisite(s): BIO 112 and 131.
Course Learning Outcomes:
Upon successful completion of the course, the student will be able to:
1.
2.
3.
4.
Describe the objectives of the Human Genome Project and potential benefits to society.
Summarize the history of recombinant DNA technology and its impact on biology research.
Demonstrate proficiency in a variety of standard biotechnology skills.
Design, conduct, and present a research project.
Outline:
I.
II.
III.
Biosciences: Biotechnology, Genetics, Toxicology, and Bioengineering
A. The Human Genome Project
B. History of recombinant DNA technology and its impact on modern biology
C. Ethical, legal, and social issues emerging from the human genome project and
biotechnology
D. Examine careers in biotechnology
Biotechnology Lab Skills
A. Standard laboratory technique proficiency in:
1. Metric measurement of volume
2. Mass and length
3. Gel electrophoresis
4. PCR
5. Bioseparations
6. Transformations
B. Techniques following a procedure:
1. Pipetting
2. Making solutions
3. Sterile technique
4. Transformation
5. Subcloning
6. Restriction enzyme analysis
7. Gel electrophoresis
8. PCR
9. Protein purification
C. Key techniques used in recombinant DNA technology
D. Key techniques in the studies of the Human Genome
Scientific Methods to Formulate and Answer Questions
A.
B.
C.
Reading research journal articles
Designing and conducting a research project
Preparing and presenting research results