Download Electrophoresis Virtual Lab

Name______________________________
Date_________Per_____
GENETIC SCIENCE LEARNING CENTER website: Gel Electrophoresis Virtual Lab
http://learn.genetics.utah.edu/content/labs/gel/
1) What is gel electrophoresis used for?
__________________________________________________________________________________________
2) What molecules can be separated by gel electrophoresis?
__________________________________________________________________________________________
3) Describe what an (agarose) gel is:
__________________________________________________________________________________________
4) We place ____________ samples into ____________ at one end of the gel.
5) By adding an ___________________________ current, we can make the DNA move.
+ + + + represent a _____________________ charge.
- - - - represent a _____________________ charge.
6) What do you see happening to the DNA in the “super close up”?
__________________________________________________________________________________________
7) Short strands move through the gel ___________________________ than long strands.
8) The shorter strands will move ___________________________ from the starting point than the longer
strands.
9) DNA strands of the same _____________________ will move at the same _____________________.
10) Staining the sorted groups of DNA makes them ___________________________to the naked eye, which
shows them as ___________________________ in the DNA.
11) List the five steps to “run a gel”:
Step 1______________________________________________
Step 2______________________________________________
Step 3______________________________________________
Step 4______________________________________________
Step 5______________________________________________
12) Here is what you will need to make a gel:
___________________________
___________________________
___________________________
___________________________
___________________________
___________________________
13) Agarose powder is made from ___________________________.
14) The buffer is____________________________________________________________________________.
15) The comb leaves empty wells (or “holes”) in the cooled gel for the _______________________.
16) The buffer ______________________________________________________________________ and also
__________________________________________________________________________________________
17) What are the two purposes of loading buffer?
______________________________________________
______________________________________________
18) Loading DNA samples into the wells accurately takes ______________________________ !!!
19) The DNA size standard contains____________________________________________________________.
20) Running it (the DNA size standard) on the gel will give you a reference by which to :
__________________________________________________________________________________________
21) The black end will generate a ______________________________ charge.
22) The red end will generate a ______________________________ charge.
23) DNA has a ______________________________ charge.
24) To move the DNA through the gel, you must place the ____________________ cord closest to the wells.
25) ______________________________ are proof that the current is running.
26) We can’t actually see the migrating DNA bands, but we can see the _________________________ from the
_________________________ as it migrates.
27) In the animation, ethidium bromide is used to stain the DNA, which will show up as _________________ in
the gel. In class we will be using a DNA stain called "Fast Blast:.
28) What does bp stand for?________________________________________
29) How do you use the DNA size standard to determine the length (size) of the bands in your DNA sample?
__________________________________________________________________________________________
30) Estimate the size of each band in the animation:
Estimated Size:
Actual Size:
_________bp
_________bp
_________bp
_________bp
_________bp
_________bp
Explore Can DNA Demand a Verdict http://learn.genetics.utah.edu/content/labs/gel/forensics/