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wk3
11
DE–4669
DISTANCE EDUCATION
M.Phil.(Botany) DEGREE EXAMINATION,
DECEMBER 2008.
Non–Semester
RESEARCH METHODOLOGY
(2006 batch)
Time : Three hours
Maximum : 100 marks
Answer any FIVE questions.
1.
Explain the principle and method of usage of phase
contrast microscope.
2.
How cytological tools and techniques are useful for the
botanical research?
3.
Explain the process of separation of phytochemicals by
electrophoresis.
4.
Describe RFLP and RAPD.
5.
Give a collective account on Blotting techniques.
6.
Describe the method of construction of Agrobacterium
strain for gene cloning and explain the formation of transgenic
plants.
7.
“Chi-square test as a test of goodness of fit” – Explain
with example.
8.
Explain pair wise alignment and multiple alignment.
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wk3
12
DE–4670
DISTANCE EDUCATION
M.Phil. (NS) (Botany) DEGREE EXAMINATION,
DECEMBER 2008.
PLANT TAXONOMY
(2006 Batch)
Time : Three hours
Maximum : 100 marks
Answer any FIVE questions.
1.
Explain
systematics.
the
structural,
biological
and
molecular
2.
Write the scope of plant taxonomy and mention about the
species concept.
3.
Compare the classification of Engler and Prantl with
Hutchinson’s classification.
4.
Summarize the classification of Takhtajan.
5.
State the principles, steps involved, advantages and
applications of numerical taxonomy.
6.
Enumerate the principles and rules of nomenclature.
7.
Discuss the anatomical, cytological, embryological and
palynological features in relation to taxonomy.
8.
Give a
biodiversity.
detailed
account
on
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2
the
conservation
of
wk3
13
DE–4671
DISTANCE EDUCATION
M.Phil. (Botany) DEGREE EXAMINATION,
DECEMBER 2008.
(Non–Semester)
PLANT BIOTECHNOLOGY
(2006 batch)
Time : Three hours
Maximum : 100 marks
Answer any FIVE questions.
1.
Write an account on plant regeneration by induction of
callus culture and its application. Explain the role of various
factors on callus induction.
2.
Describe the status of plant tissue culture research in
India with particular emphasis on micro propagation and germ
plasm storage.
3.
Describe in detail about the schemes for obtaining
somoclonal variation and its application.
4.
Describe the methods used for induction of protoplast
fusion leading to production of somatic hybrids. Discuss the
utility of this technique in plant breeding.
5.
Discuss the targeting of foreign proteins into chloroplasts
and mitochondria.
6.
Describe the ‘co-integrate’ and ‘binary’ vectors
constructed using Ti and Ri plasmids of Agro bacterium.
Discuss their relative merits and demerits.
3
wk3
7.
Discuss the method and significance of gene tagging
using molecular markers. Give a list of some major genes of
economic value, which have been tagged.
8.
Write an essay on the production of any two transgenic
plants you have studied.
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