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Supplementary figure captions
Supplementary Figure S1
Analysis of significant gene expression profiles in the IAP-expressing strain C.U17 and the reference strain C.WT
over time. The identified genes (1004) were grouped in 12 clusters according to the average gene expression of
C.U17 (IAP, red) and C.WT (WT, green), and the median profile of the sampled time points in the chemostat
cultures (duplicate) is presented. Three clusters (A, B, C) were identified, which showed different averaged
expression profile between the strains C.U17 and CWT (see main text).
Supplementary Figure S2:
Time-course analysis of intracellular amino acid profiles in the IAP-expressing strain C.U17 and the reference
strain C.WT in chemostat cultivations. The amino acid concentrations in C.U17 (IAP, orange) and C.WT (WT, black)
were log2-scaled and normalized to the initial concentration of the analyzed amino acid at early steady state (t=
135 h) for each strain, thus highlighting their fold change over time. In alphabetic order: ALA, alanine; ASN,
asparagine; ASP, aspartate; CYC, cysteine; GLN, glutamine; GLU, glutamate; GLY, glycine; HIS, histidine; ILE,
isoleucine; LEU, leucine; LYS, lysine; MET, methionine; ORN, ornithine; PRO, proline; PHE, phenylalanine; SER,
serine; THR, threonine; TRP, tryptophan; TYR, tyrosine; VAL, valine
Supplementary Figure S3:
Heatmaps illustrating the normalized (mean = 0, variance = 1) and scaled (-2 to 2) expression levels of
hierarchically clustered genes in the IAP-expressing strain C.U17, which are related to amino acid biosynthesis.
The critical process phase (pcrit) is indicated. The heatmaps are grouped according to the amino acid super-pathway
classification of Yeast Biochemical Pathway Database (YeastCyc).
Supplementary Figure S4:
Heatmaps illustrating the normalized (mean = 0, variance = 1) and scaled (-2 to 2) expression levels of
hierarchically clustered genes in the IAP-expressing strain C.U17, which are related to glycolysis, tricarboxylic
acid (TCA) cycle, pentose phosphate pathway (PPP), and storage metabolism. The critical process phase (pcrit) is
indicated. The heatmaps are grouped according to the Gene Ontology (GO) biological process classification in
Saccharomyces Genome Database (SGD).
Supplementary Figure S5:
Time-course analysis of intracellular profiles of central carbon metabolite in the IAP-expressing strain C.U17 and
the reference strain C.WT in chemostat cultivations. The metabolite concentrations in C.U17 (IAP, green) and
C.WT (WT, black) were log2-scaled and normalized to the initial concentration of the analyzed metabolite at early
steady state (t= 135 h) for each strain, thus highlighting their fold change over time. Error bars represent standard
deviation in two replicate chemostat cultures of each strain. In alphabetic order: CS, residual glucose; CIT, citrate;
F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; G6P, glucose-6-phosphate; GAP, glyceraldehyde-3phosphate; MAL, malate; PYR, pyruvate; PEP, phosphoenolpyruvate; SUC, succinate; 2PG/3PG, pool of 2P-glycerate
and 3P-glycerate; α-KG, α-ketoglutarate.
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